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Current Stem Cell Research & Therapy

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ISSN (Print): 1574-888X
ISSN (Online): 2212-3946

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Research Article

Rg1 Promotes the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells via FXR1/Lnc-GAS5-AS1 Pathway

Author(s): Fang-Tian Xu , Yin-Li Xu, Yong-Xian Rong, Dong-Lin Huang, Zhong-Hong Lai, Xin-Heng Liu, Ling-Hui Yang, Steven Mo*, Zheng-Qiu Wu* and Hong-Mian Li*

Volume 17, Issue 8, 2022

Published on: 28 December, 2021

Page: [815 - 824] Pages: 10

DOI: 10.2174/1574888X16666211129121414

Price: $65

Abstract

Background: Human adipose-derived stem cells (hASCs) play an important role in regenerative medicine.

Objective: Exploring the mechanism of Rg1 in the promotion of the proliferation and adipogenic differentiation of hASCs is important in regenerative medicine research.

Methods: To observe ginsenoside Rg1 in promoting the proliferation and adipogenic differentiation of hASCs, Rg1 medium at different concentrations was established and tested using the cell counting kit-8 (CCK-8) assay, oil red O staining, alizarin red, and alcian blue. Compared to the control, differentially expressed genes (DEGs) were screened via DEG analysis, which was carried out in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. To explore the relationship among mRNA, long non-coding RNA (lncRNA) and microRNA (miRNA), we constructed a competing endogenous RNA (ceRNA) network.

Results: In this study, Rg1 was observed to promote the proliferation and adipogenic differentiation of hASCs. Additionally, enriched BPs and KEGG pathways may be involved in the promotion process, where FXR1 and Lnc-GAS5-AS1 were found to be regulatory factors. The regulatory network suggested that Rg1 could regulate the adipocytokine signaling pathway and IL−17 signaling pathway via FXR1 and Lnc-GAS5-AS1, which served as the mechanism encompassing the promotion of Rg1 on the proliferation and adipogenic differentiation of hASCs.

Conclusion: A comprehensive transcriptional regulatory network related to the promotion ability of Rg1 was constructed, revealing mechanisms regarding Rg1’s promotion of the proliferation and adipogenic differentiation of hASCs. The present study provides a theoretical basis for optimizing the function of hASCs.

Keywords: Human adipose-derived stem cells, ginsenoside Rg1, proliferation, adipogenic differentiation, regenerative medicine, theoretical basis.

« Previous Next »
[1]
Cho EH, Shammas RL, Carney MJ, et al. Muscle versus fasciocutaneous free flaps in lower extremity traumatic reconstruction: A multicenter outcomes analysis. Plast Reconstr Surg 2018; 141(1): 191-9.
[http://dx.doi.org/10.1097/PRS.0000000000003927] [PMID: 28938362]
[2]
Teresa Minjung O, Chan K, Brennan T, et al. Autologous fat grafting restores soft-tissue contour deformities after vascular anomaly surgery. Plast Reconstr Surg Glob Open 2019; 7(5): e2196.
[http://dx.doi.org/10.1097/GOX.0000000000002196] [PMID: 31333935]
[3]
Zamborsky R, Kilian M, Jacko P, Bernadic M, Hudak R. Perspectives of 3D printing technology in orthopaedic surgery. Bratisl Lek Listy 2019; 120(7): 498-504.
[http://dx.doi.org/10.4149/BLL_2019_079] [PMID: 31602984]
[4]
Cohen Tervaert JW, Colaris MJ, van der Hulst RR. Silicone breast implants and autoimmune rheumatic diseases: Myth or reality. Curr Opin Rheumatol 2017; 29(4): 348-54.
[http://dx.doi.org/10.1097/BOR.0000000000000391] [PMID: 28379860]
[5]
Kontoes P, Gounnaris G. Complications of fat transfer for breast augmentation. Aesthetic Plast Surg 2017; 41(5): 1078-82.
[http://dx.doi.org/10.1007/s00266-017-0911-2] [PMID: 28643006]
[6]
Bourin P, Bunnell BA, Casteilla L, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy 2013; 15(6): 641-8.
[http://dx.doi.org/10.1016/j.jcyt.2013.02.006] [PMID: 23570660]
[7]
Almalki SG, Agrawal DK. Key transcription factors in the differentiation of mesenchymal stem cells. Differentiation 2016; 92(1-2): 41-51.
[http://dx.doi.org/10.1016/j.diff.2016.02.005] [PMID: 27012163]
[8]
Zheng HZ, Fu XK, Shang JL, Lu RX, Ou YF, Chen CL. Ginsenoside Rg1 protects rat bone marrow mesenchymal stem cells against ischemia induced apoptosis through miR-494-3p and ROCK-1. Eur J Pharmacol 2018; 822: 154-67.
[http://dx.doi.org/10.1016/j.ejphar.2018.01.001] [PMID: 29307726]
[9]
Liang ZJ, Lu X, Zhu DD, et al. Ginsenoside Rg1 accelerates paracrine activity and adipogenic differentiation of human breast adipose-derived stem cells in a dose-dependent manner in vitro. Cell Transplant 2019; 28(3): 286-95.
[http://dx.doi.org/10.1177/0963689719825615] [PMID: 30675799]
[10]
Xu FT, Li HM, Yin QS, et al. Effect of activated autologous platelet-rich plasma on proliferation and osteogenic differentiation of human adipose-derived stem cells in vitro. Am J Transl Res 2015; 7(2): 257-70.
[PMID: 25901195]
[11]
Li H, Handsaker B, Wysoker A, et al. The sequence alignment/map format and samtools. Bioinformatics 2009; 25(16): 2078-9.
[http://dx.doi.org/10.1093/bioinformatics/btp352] [PMID: 19505943]
[12]
Ritchie ME, Phipson B, Wu D, et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res 2015; 43(7): e47.
[http://dx.doi.org/10.1093/nar/gkv007] [PMID: 25605792]
[13]
Yu G, Wang LG, Han Y, He QY. clusterProfiler: An R package for comparing biological themes among gene clusters. OMICS 2012; 16(5): 284-7.
[http://dx.doi.org/10.1089/omi.2011.0118] [PMID: 22455463]
[14]
Subramanian A, Tamayo P, Mootha VK, et al. Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA 2005; 102(43): 15545-50.
[http://dx.doi.org/10.1073/pnas.0506580102] [PMID: 16199517]
[15]
Liberzon A, Birger C, Thorvaldsdóttir H, Ghandi M, Mesirov JP, Tamayo P. The molecular signatures database (msigdb) hallmark gene set collection. Cell Syst 2015; 1(6): 417-25.
[http://dx.doi.org/10.1016/j.cels.2015.12.004] [PMID: 26771021]
[16]
Lin Y, Liu T, Cui T, et al. RNAInter in 2020: RNA interactome repository with increased coverage and annotation. Nucleic Acids Res 2020; 48(D1): D189-97.
[http://dx.doi.org/10.1093/nar/gkz804] [PMID: 31906603]
[17]
Han H, Cho JW, Lee S, et al. TRRUST v2: An expanded reference database of human and mouse transcriptional regulatory interactions. Nucleic Acids Res 2018; 46(D1): D380-6.
[http://dx.doi.org/10.1093/nar/gkx1013] [PMID: 29087512]
[18]
Shannon P, Markiel A, Ozier O, et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res 2003; 13(11): 2498-504.
[http://dx.doi.org/10.1101/gr.1239303] [PMID: 14597658]
[19]
Rizvi SM, Shakil S, Haneef M. A simple click by click protocol to perform docking: AutoDock 4.2 made easy for non-bioinformaticians. EXCLI J 2013; 12: 831-57.
[PMID: 26648810]
[20]
Uciechowska-Kaczmarzyk U, Chauvot de Beauchene I, Samsonov SA. Docking software performance in protein-glycosaminoglycan systems. J Mol Graph Model 2019; 90: 42-50.
[http://dx.doi.org/10.1016/j.jmgm.2019.04.001] [PMID: 30959268]
[21]
Si Z, Wang X, Sun C, et al. Adipose-derived stem cells: Sources, potency, and implications for regenerative therapies. Biomed Pharmacother 2019; 114: 108765.
[http://dx.doi.org/10.1016/j.biopha.2019.108765] [PMID: 30921703]
[22]
Li W, Liu Y, Zhang P, et al. Tissue-engineered bone immobilized with human adipose stem cells-derived exosomes promotes bone regeneration. ACS Appl Mater Interfaces 2018; 10(6): 5240-54.
[http://dx.doi.org/10.1021/acsami.7b17620] [PMID: 29359912]
[23]
Bai M, Zhang L, Fu B, et al. IL-17A improves the efficacy of mesenchymal stem cells in ischemic-reperfusion renal injury by increasing Treg percentages by the COX-2/PGE2 pathway. Kidney Int 2018; 93(4): 814-25.
[http://dx.doi.org/10.1016/j.kint.2017.08.030] [PMID: 29132705]
[24]
Farouk S, Sabet S, Abu Zahra FA, El-Ghor AA. Bone marrow derived-mesenchymal stem cells downregulate IL17A dependent IL6/STAT3 signaling pathway in CCl4-induced rat liver fibrosis. PLoS One 2018; 13(10): e0206130.
[http://dx.doi.org/10.1371/journal.pone.0206130] [PMID: 30346985]
[25]
He Y, Guo Y, Xia Y, et al. Resistin promotes cardiac homing of mesenchymal stem cells and functional recovery after myocardial ischemia-reperfusion via the ERK1/2-MMP-9 pathway. Am J Physiol Heart Circ Physiol 2019; 316(1): H233-44.
[http://dx.doi.org/10.1152/ajpheart.00457.2018] [PMID: 30412442]
[26]
Khandjian EW. Biology of the fragile X mental retardation protein, an RNA-binding protein. Biochem Cell Biol 1999; 77(4): 331-42.
[http://dx.doi.org/10.1139/o99-035] [PMID: 10546896]
[27]
Davidovic L, Durand N, Khalfallah O, et al. A novel role for the RNA-binding protein FXR1P in myoblasts cell-cycle progression by modulating p21/Cdkn1a/Cip1/Waf1 mRNA stability. PLoS Genet 2013; 9(3): e1003367.
[http://dx.doi.org/10.1371/journal.pgen.1003367] [PMID: 23555284]
[28]
Cao S, Zheng J, Liu X, et al. FXR1 promotes the malignant biological behavior of glioma cells via stabilizing MIR17HG. J Exp Clin Cancer Res 2019; 38(1): 37.
[http://dx.doi.org/10.1186/s13046-018-0991-0] [PMID: 30691465]
[29]
Majumder M, Palanisamy V. RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer. PLoS Genet 2020; 16(1): e1008580.
[http://dx.doi.org/10.1371/journal.pgen.1008580] [PMID: 31940341]
[30]
Wu Y, Lyu H, Liu H, Shi X, Song Y, Liu B. Downregulation of the long noncoding RNA GAS5-AS1 contributes to tumor metastasis in non-small cell lung cancer. Sci Rep 2016; 6: 31093.
[http://dx.doi.org/10.1038/srep31093] [PMID: 27489122]
[31]
Wang X, Zhang J, Wang Y. Long noncoding RNA GAS5-AS1 suppresses growth and metastasis of cervical cancer by increasing GAS5 stability. Am J Transl Res 2019; 11(8): 4909-21.
[PMID: 31497208]

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