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Current Drug Delivery

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ISSN (Print): 1567-2018
ISSN (Online): 1875-5704

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Research Article

Adsorption of Cisplatin on Oxidized Graphene Nanoribbons for Improving the Uptake in Non-small Cell Lung Carcinoma Cell Line A549

Author(s): Steffi Augustine, Bala Prabhakar and Pravin Shende*

Volume 19, Issue 6, 2022

Published on: 10 January, 2022

Page: [697 - 705] Pages: 9

DOI: 10.2174/1567201818666210708124424

Price: $65

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Abstract

Background: Graphene nanoribbons are nanosized strips of graphene with unique physicochemical properties like higher drug loading capacity and affinity for tumor cells.

Objective: The principal objective of this research was to develop oxidized graphene nanoribbons (O-GNRs)-based delivery system for cisplatin against non-small cell lung carcinoma cell line A549 by selective endocytosis.

Methods: The O-GNRs prepared using various synthetic steps like oxidative unzipping were evaluated for various parameters like morphology, Fourier Transform Infrared (FTIR) study, % adsorption efficacy, Differential scanning colometric (DSC) study and in-vitro efficacy studies.

Results: Graphene nanoribbons with the length of 200-250 nm and width of 20-40 nm were obtained. The FTIR spectrum of drug-loaded O-GNRs exhibited a characteristic peak at 1550 cm-1 (- N-H group) of cisplatin. The DSC indicated the presence of sharp endothermic peaks at 59°C (PEG), 254°C (-C-NH3) and 308.6°C (-C-Pt). The % adsorption efficiency was found to be 74.56 ± 0.798% with in-vitro release in controlled manner (63.36% ± 0.489%) for 24 h.

Conclusion: The nanoformulation showed an average inhibition of 22.72% at a lower dose of cisplatin (> 25%) by passive targeting on cell line A549 by DNA alkylation. In the near future, graphene-based systems will establish potential nanosystems in cancer treatment due to the additive effect of graphene with various therapeutic agents.

Keywords: Graphene nanoribbons, PEGylation, unzipping, multi-walled carbon nanotubes, endocytosis, tumor cells.

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[1]
Barrera-Rodriguez R, Morales-Fuentes J. Lung cancer in women. Lung Cancer (Auckl) 2012; 3: 79-89.
[http://dx.doi.org/10.2147/LCTT.S37319] [PMID: 28210127]
[2]
Prabhakar B, Shende P, Augustine S. Current trends and emerging diagnostic techniques for lung cancer. Biomed Pharmacother 2018; 106: 1586-99.
[http://dx.doi.org/10.1016/j.biopha.2018.07.145] [PMID: 30119234]
[3]
Shende P, Patil S, Gaud RS. A combinatorial approach of inclusion complexation and dendrimer synthesization for effective targeting EGFR-TK. Mater Sci Eng C 2017; 76: 959-65.
[http://dx.doi.org/10.1016/j.msec.2017.03.184] [PMID: 28482613]
[4]
Paratala BS, Jacobson BD, Kanakia S, Francis LD, Sitharaman B. Physicochemical characterization, and relaxometry studies of micro-graphite oxide, graphene nanoplatelets, and nanoribbons. PLoS One 2012; 7(6): e38185.
[http://dx.doi.org/10.1371/journal.pone.0038185] [PMID: 22685555]
[5]
McCallion C, Burthem J, Rees-Unwin K, Golovanov A, Pluen A. Graphene in therapeutics delivery: Problems, solutions and future opportunities. Eur J Pharm Biopharm 2016; 104: 235-50.
[http://dx.doi.org/10.1016/j.ejpb.2016.04.015] [PMID: 27113141]
[6]
Liu Z, Robinson JT, Sun X, Dai H. PEGylated nanographene oxide for delivery of water-insoluble cancer drugs. J Am Chem Soc 2008; 130(33): 10876-7.
[http://dx.doi.org/10.1021/ja803688x] [PMID: 18661992]
[7]
Xu Z, Zhu S, Wang M, Li Y, Shi P, Huang X. Delivery of paclitaxel using PEGylated graphene oxide as a nanocarrier. ACS Appl Mater Interfaces 2015; 7(2): 1355-63.
[http://dx.doi.org/10.1021/am507798d] [PMID: 25546399]
[8]
Terronesa M, Andres R, Campos-Delgado J, et al. Graphene and graphite nanoribbons: Morphology, properties, synthesis, defects and application. Nano Today 2010; 5: 351-72.
[http://dx.doi.org/10.1016/j.nantod.2010.06.010]
[9]
Kosynkin DV, Higginbotham AL, Sinitskii A, et al. Longitudinal unzipping of carbon nanotubes to form graphene nanoribbons. Nature 2009; 458(7240): 872-6.
[http://dx.doi.org/10.1038/nature07872] [PMID: 19370030]
[10]
Xiao B, Li X, Li X, et al. Graphene nanoribbons derived from the unzipping of carbon nanotubes: controlled synthesis and superior lithium storage performance. J Phys Chem C 2013; 118: 881-90.
[http://dx.doi.org/10.1021/jp410812v]
[11]
Kim YJ, Park HC, Kim BK. Triple shape-memory effect by silanized polyurethane/silane-functionalized graphene oxide nanocomposites bilayer. High Perform Polym 2015; 27: 886-97.
[http://dx.doi.org/10.1177/0954008314565398]
[12]
Cong HP, He JJ, Lu Y, Yu SH. Water-soluble magnetic-functionalized reduced graphene oxide sheets: in situ synthesis and magnetic resonance imaging applications. Small 2010; 6(2): 169-73.
[http://dx.doi.org/10.1002/smll.200901360] [PMID: 19885891]
[13]
Keller JW. Sulfur dioxide–pyridine dimer. FTIR and theoretical evidence for a low-symmetry structure. J Phys Chem A 2015; 119(41): 10390-8.
[http://dx.doi.org/10.1021/acs.jpca.5b06122] [PMID: 26401726]
[14]
Jiao L, Wang X, Diankov G, Wang H, Dai H. Facile synthesis of high-quality graphene nanoribbons. Nat Nanotechnol 2010; 5(5): 321-5.
[http://dx.doi.org/10.1038/nnano.2010.54] [PMID: 20364133]
[15]
Liao Z, Medrano Sandonas L, Zhang T, et al. in-situ stretching patterned graphene nanoribbons in the transmission electron microscope. Sci Rep 2017; 7(1): 211.
[http://dx.doi.org/10.1038/s41598-017-00227-3] [PMID: 28303001]
[16]
Lu YJ, Lin CW, Yang HW, et al. Biodistribution of PEGylated graphene oxide nanoribbons and their application in cancer chemo-photothermal therapy. Carbon 2014; 74: 83-95.
[http://dx.doi.org/10.1016/j.carbon.2014.03.007]
[17]
Johnson DW, Dobson BP, Coleman KS. A manufacturing perspective on graphene dispersions. Curr Opin Colloid Interface Sci 2015; 20: 367-82.
[http://dx.doi.org/10.1016/j.cocis.2015.11.004]
[18]
Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc 2006; 1(3): 1112-6.
[http://dx.doi.org/10.1038/nprot.2006.179] [PMID: 17406391]
[19]
Skehan P, Storeng R, Scudiero D, et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 1990; 82(13): 1107-12.
[http://dx.doi.org/10.1093/jnci/82.13.1107] [PMID: 2359136]
[20]
Polu RA, Kuma R. Impedance spectroscopy and FTIR studies of PEG-based polymer electrolytes. J Chem 2011; 8: 347-53.
[21]
Shende P, Vaidya J, Kulkarni YA. Bio-inspired nano-engineered strip for semiquantitative FeNO analysis. J Breath Res 2019; 13(4): 046002.
[http://dx.doi.org/10.1088/1752-7163/ab1faf] [PMID: 31063980]
[22]
Hu X, Yu Y, Hou W, Zhou J, Song L. Effects of particle size and pH value on the hydrophilicity of graphene oxide. Appl Surf Sci 2013; 273: 118-21.
[http://dx.doi.org/10.1016/j.apsusc.2013.01.201]
[23]
Shende P, Vaidya J, Kulkarni YA, Gaud RS. Systematic approaches for biodiagnostics using exhaled air. J Control Release 2017; 268: 282-95.
[http://dx.doi.org/10.1016/j.jconrel.2017.10.035] [PMID: 29111149]
[24]
Celis A, Nair MV, Taleb-Ibrahimi A, et al. Graphene nanoribbons: Fabrication, properties and devices. JPhy D 2016; 49: 143001.
[http://dx.doi.org/10.1088/0022-3727/49/14/143001]
[25]
Moore TL, Podilakrishna R, Rao A, Alexis F. Systemic administration of polymer-coated nano-graphene to deliver drugs to glioblastoma. Part Part Syst Charact 2014; 31(8): 886-94.
[http://dx.doi.org/10.1002/ppsc.201300379]
[26]
Dreyer DR, Park S, Bielawski CW, Ruoff RS. The chemistry of graphene oxide. Chem Soc Rev 2010; 39(1): 228-40.
[http://dx.doi.org/10.1039/B917103G] [PMID: 20023850]
[27]
Kim H, Kim WJ. Photothermally controlled gene delivery by reduced graphene oxide-polyethylenimine nanocomposite. Small 2014; 10(1): 117-26.
[http://dx.doi.org/10.1002/smll.201202636] [PMID: 23696272]
[28]
Sahay G, Alakhova DY, Kabanov AV. Endocytosis of nanomedicines. J Control Release 2010; 145(3): 182-95.
[http://dx.doi.org/10.1016/j.jconrel.2010.01.036] [PMID: 20226220]
[29]
Chaiyakun S, Witit-Anun N, Nuntawong N, et al. Preparation and characterization of graphene oxide nanosheets. Procedia Eng 2012; 32: 759-64.
[http://dx.doi.org/10.1016/j.proeng.2012.02.009]
[30]
Yang X, Zhang X, Liu Z, Ma Y, Huang Y, Chen Y. High-efficiency loading and controlled release of doxorubicin hydrochloride on graphene oxide. J Phys Chem C 2008; 122: 17554-8.
[http://dx.doi.org/10.1021/jp806751k]
[31]
Nandi A, Mallick A, More P, Sengupta P, Ballav N, Basu S. Cisplatin-induced self-assembly of graphene oxide sheets into spherical nanoparticles for damaging sub-cellular DNA. Chem Commun (Camb) 2017; 53(8): 1409-12.
[http://dx.doi.org/10.1039/C6CC09006K] [PMID: 28079217]
[32]
Mero A, Pasut G, Veronese FM. A useful technology in anticancer therapy. PEGylated protein drugs: Basic science and clinical applications 2009; 255-71.
[http://dx.doi.org/10.1007/978-3-7643-8679-5_15]
[33]
Shende P, Pathan N. Graphene nanoribbons: A state-of-the-art in health care. Intern J Pharm 2021; 120269.

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