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Combinatorial Chemistry & High Throughput Screening

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ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

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Research Article

Anti-mycobacterial Activity of 22 Iranian Endemic or Rare Plant Extracts Against Multi-drug and Extensively Drug-resistant Mycobacterium tuberculosis

Author(s): Sina Dehestani, Javid Davoodi, Seyed Ahmad Emami, Fereshteh Sadat Seddighinia, Amirhossein Sahebkar* and Saman Soleimanpour*

Volume 25, Issue 5, 2022

Published on: 15 February, 2021

Page: [870 - 876] Pages: 7

DOI: 10.2174/1386207324666210215100931

Price: $65

Abstract

Background: Due to side-effects and low efficacy of common drugs on new resistant strains of Mycobacterium tuberculosis (Mtb), investigation on novel drugs and natural compounds from rich sources of endemic plants is required. Thus, in the present study, the anti-mycobacterial effects of 22 Iranian endemic or rare plant extracts on multi-drug resistance (MDR) and extensively-drug resistance (XDR) Mtb isolates were evaluated.

Methods: Twenty-two Iranian endemic and rare plant species from 9 families were collected and extracted by methanol. Their inhibitory-effects were then evaluated against Mtb H37Rv strain, seven clinical MDR-TB, and two XDR-TB isolates using the resazurin microtiter assay (REMA) method. The best of them were then fractionalized by five different polar solvents (Petroleum- Ether, Dichloromethane, Ethyl-Acetate, n-butanol, and water). To find anti-mycobacterial fractions, the inhibitory effect of isolated fractions was tested on Mtb H37Rv.

Results: Out of the 22 plants, 14 plant extracts demonstrated anti-mycobacterial activity with minimum inhibitory concentration (MIC) ranging from 4 to 30μg/mL against Mtb H37Rv. Eight plant extracts also exhibited anti-mycobacterial activity against MDR and XDR clinical strains of Mtb by MICs, i.e., 15-60μg/ml. Crinitaria grimmii and Linum album were the best antimycobacterial plants. Among fractions of Crinitaria grimmii, dichloromethane and n-butanol, and for Linum album, dichloromethane and Ethyl-Acetate fractions displayed more anti-mycobacterial effect as compared to crude extract on Mtb.

Conclusion: The present study confirms the potential role of some plants to treat respiratory diseases as our results have demonstrated that these plants exhibit anti-mycobacterial activity in the acceptable range against Mtb. Thus, these plants could be good sources and alternatives of plant metabolites for anti-TB-drug development.

Keywords: Iranian endemic plants, Mycobacterium tuberculosis, tuberculosis, MDR-TB, XDR-TB, MIC.

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[1]
Organization, W.H. Global tuberculosis report, 2016. 2016.
[2]
Jimenez-Arellanes, A.; Meckes, M.; Ramirez, R.; Torres, J.; Luna-Herrera, J. Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases. Phytother. Res., 2003, 17(8), 903-908.
[http://dx.doi.org/10.1002/ptr.1377] [PMID: 13680821]
[3]
Organization, W.H. Global tuberculosis report, 2017. 2017.
[4]
Lall, N.; Meyer, J.J. In vitro inhibition of drug-resistant and drug-sensitive strains of Mycobacterium tuberculosis by ethnobotanically selected South African plants. J. Ethnopharmacol., 1999, 66(3), 347-354.
[http://dx.doi.org/10.1016/S0378-8741(98)00185-8] [PMID: 10473184]
[5]
Gupta, V.K.; Shukla, C.; Bisht, G.R.; Saikia, D.; Kumar, S.; Thakur, R.L. Detection of anti-tuberculosis activity in some folklore plants by radiometric BACTEC assay. Lett. Appl. Microbiol., 2011, 52(1), 33-40.
[http://dx.doi.org/10.1111/j.1472-765X.2010.02963.x] [PMID: 21114505]
[6]
Jyoti, M.A.; Nam, K.W.; Jang, W.S.; Kim, Y.H.; Kim, S.K.; Lee, B.E.; Song, H.Y. Antimycobacterial activity of methanolic plant extract of Artemisia capillaris containing ursolic acid and hydroquinone against Mycobacterium tuberculosis. J. Infect. Chemother., 2016, 22(4), 200-208.
[http://dx.doi.org/10.1016/j.jiac.2015.11.014] [PMID: 26867795]
[7]
Tosun, F.; Kizilay, C.A.; Sener, B.; Vural, M.; Palittapongarnpim, P. Antimycobacterial screening of some Turkish plants. J. Ethnopharmacol., 2004, 95(2-3), 273-275.
[http://dx.doi.org/10.1016/j.jep.2004.07.011] [PMID: 15507348]
[8]
Bailey, C.J.; Day, C. Traditional plant medicines as treatments for diabetes. Diabetes Care, 1989, 12(8), 553-564.
[http://dx.doi.org/10.2337/diacare.12.8.553] [PMID: 2673695]
[9]
Dominguez, D.M. Pyrrolizidine alkaloids from Canarian endemic plants and their biological effects. Biochem. Syst. Ecol., 2008, 36(3), 153-166.
[http://dx.doi.org/10.1016/j.bse.2007.08.015]
[10]
Khanavi, M.; Vatandoost, H.; Khosravi Dehaghi, N.; Sanei Dehkordi, A.; Sedaghat, M.M.; Hadjiakhoondi, A.; Hadjiakhoondi, F. Larvicidal activities of some Iranian native plants against the main malaria vector, Anopheles stephensi. Acta Med. Iran., 2013, 51(3), 141-147.
[PMID: 23605596]
[11]
Ebrahimi, A.; Asgharian, S.; Habibian, S. Antimicrobial activities of isolated endophytes from some Iranian native medicinal plants. Indian J. Pharm. Sci., 2010, 6(3), 217-222.
[12]
Obonga, W.O. Comparative Phytochemical, Antioxidant and Antimicrobial Properties of Ficus capensis, Aristolochia ringens, Albizia zygia and Lannea welwitschii. Dhaka University Journal of Pharmaceutical Sciences, 16(2), 147-157.
[13]
Ghasemi, Y. Ferula gummosa fruits: an aromatic antimicrobial agent. Chem. Nat. Compd., 2005, 41(3), 311-314.
[http://dx.doi.org/10.1007/s10600-005-0138-3]
[14]
Al-Shamma, A.; Mitscher, L.A. Comprehensive survey of indigenous Iraqi plants for potential economic value. 1. Screening results of 327 species for alkaloids and antimicrobial agents. J. Nat. Prod., 1979, 42(6), 633-642.
[http://dx.doi.org/10.1021/np50006a009] [PMID: 541687]
[15]
Schmidt, T.J.; Klaes, M.; Sendker, J. Lignans in seeds of Linum species. Phytochemistry, 2012, 82, 89-99.
[http://dx.doi.org/10.1016/j.phytochem.2012.07.004] [PMID: 22854497]
[16]
Palomino, J-C.; Martin, A.; Camacho, M.; Guerra, H.; Swings, J.; Portaels, F. Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother., 2002, 46(8), 2720-2722.
[http://dx.doi.org/10.1128/AAC.46.8.2720-2722.2002] [PMID: 12121966]
[17]
Sarker, S.D.; Nahar, L.; Kumarasamy, Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 2007, 42(4), 321-324.
[http://dx.doi.org/10.1016/j.ymeth.2007.01.006] [PMID: 17560319]
[18]
Schwalbe, R.; Steele-Moore, L.; Goodwin, A.C. Antimicrobial susceptibility testing protocols; Crc Press, 2007.
[http://dx.doi.org/10.1201/9781420014495]
[19]
Cantrell, C.L.; Franzblau, S.G.; Fischer, N.H. Antimycobacterial plant terpenoids. Planta Med., 2001, 67(8), 685-694.
[http://dx.doi.org/10.1055/s-2001-18365] [PMID: 11731906]
[20]
Gupta, R.; Thakur, B.; Singh, P.; Singh, H.B.; Sharma, V.D.; Katoch, V.M.; Chauhan, S.V. Anti-tuberculosis activity of selected medicinal plants against multi-drug resistant Mycobacterium tuberculosis isolates. Indian J. Med. Res., 2010, 131(6), 809-813.
[PMID: 20571171]
[21]
Sice, P. Online Screening of X-System music playlists using an integrative wellbeing model informed by the theory of autopoiesis. IEEE Access, 2020, 8, 182307-182319.
[http://dx.doi.org/10.1109/ACCESS.2020.3029142]
[22]
Al-Qudah, M.A. Chemical composition and antimicrobial activity of the essential oil of Linum pubescens growing wild in Jordan. Nat. Prod. Res., 2013, 27(12), 1141-1144.
[http://dx.doi.org/10.1080/14786419.2012.715291] [PMID: 22913459]
[23]
Javidnia, K. Biological Activity and Aryltetraline Lignans of Linum persicum. Pharm. Biol., 2005, 43(6), 547-550.
[http://dx.doi.org/10.1080/13880200500220854]
[24]
Özek, G.; Ishmuratova, M.; Tabanca, N.; Radwan, M.M.; Göger, F.; Özek, T.; Wedge, D.E.; Becnel, J.J.; Cutler, S.J.; Can Başer, K.H. One-step multiple component isolation from the oil of Crinitaria tatarica (Less.) Sojak by preparative capillary gas chromatography with characterization by spectroscopic and spectrometric techniques and evaluation of biological activity. J. Sep. Sci., 2012, 35(5-6), 650-660.
[http://dx.doi.org/10.1002/jssc.201100950] [PMID: 22331842]
[25]
Otsuka, H. Purification by solvent extraction using partition coefficient.Natural Products Isolation; Springer, 2006, pp. 269-273.
[http://dx.doi.org/10.1385/1-59259-955-9:269]

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