Login Register Cart 0
Generic placeholder image

Pharmaceutical Nanotechnology

Editor-in-Chief

ISSN (Print): 2211-7385
ISSN (Online): 2211-7393

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

The Influence of Surface Charge on the Antiviral Effect of Curcumin Loaded in Nanocarrier System

Author(s): Najwa Nabila, Siti R. Hassan, Gladys P. Larasati, Benediktus Yohan, R.T. Sasmono, Annis C. Adi, Ferry Iskandar and Heni Rachmawati*

Volume 9, Issue 3, 2021

Published on: 04 February, 2021

Page: [210 - 216] Pages: 7

DOI: 10.2174/2211738509666210204121258

Price: $65

Abstract

Background: Curcumin is a well-documented bioactive compound present in Curcuma sp., a tropical, medicinal plant. This substance exhibits broad-spectrum biological activities, including antivirus. Despite the lack of pharmaceutical properties of curcumin limits its clinical use.

Objective: This study aims to produce curcumin nanoemulsion with different surface charge (curcumin (+) nanoemulsion and curcumin (-) nanoemulsion) and to evaluate its physical characteristics, in vitro cell cytotoxicity, and antiviral activity against dengue virus (DENV) 1 and 2.

Methods: Two forms of nanoemulsion were prepared, which were differed from their surface charge through spontaneous procedure resulting in similar characteristics except for the zeta potential value. Cytotoxicity was determined using the RT-PCR method in the A549 cell line, and anti- DENV properties were determined by calculation of inhibitory concentration 50 (IC50) value.

Results: The positive charge of curcumin-loaded nanoemulsion showed a better effect in reducing the viral replication represented by a lower IC50 value. In addition, DENV-1 was more sensitive and responsive to curcumin as compared to DENV-2.

Conclusion: Positive surface charge of curcumin-loaded nanoemulsion improves the antiviral effect of the curcumin, suggesting a promising approach for alternative treatment for dengue virus infection.

Keywords: Curcumin, dengue virus, nanoemulsion, surface charge, antiviral activity, DDAB, A549 cell.

« Previous Next »
Graphical Abstract

[1]
Reynolds ES, Hart CE, Hermance ME, Brining DL, Thangamani S. An overview of animal models for arthropod-borne viruses. Comp Med 2017; 67(3): 232-41.
[PMID: 28662752]
[2]
Gubler DJ. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 1998; 11(3): 480-96.
[http://dx.doi.org/10.1128/CMR.11.3.480] [PMID: 9665979]
[3]
Martina BEE, Koraka P, Osterhaus ADME. Dengue virus pathogenesis: an integrated view. Clin Microbiol Rev 2009; 22(4): 564-81.
[http://dx.doi.org/10.1128/CMR.00035-09] [PMID: 19822889]
[4]
Póvoa TF, Alves AMB, Oliveira CA, Nuovo GJ, Chagas VLA, Paes MV. The pathology of severe dengue in multiple organs of human fatal cases: histopathology, ultrastructure and virus replication. PLoS One 2014; 9(4): e83386.
[http://dx.doi.org/10.1371/journal.pone.0083386] [PMID: 24736395]
[5]
The situation of dengue fever in Indonesia in 2017 [Situasi penyakit demam berdarah di Indonesia tahun 2017]. Indonesian Ministry of Health 2017. Available at: https://www.kemkes.go.id/resources/download/pusdatin/infodatin/InfoDatin-Situasi-Demam-Berdarah- Dengue.pdf
[6]
Low JGH, Ooi EE, Vasudevan SG. Current status of dengue therapeutics research and development. J Infect Dis 2017; 215(S2)(suppl_2): S96-S102.
[http://dx.doi.org/10.1093/infdis/jiw423] [PMID: 28403438]
[7]
Dighe SN, Ekwudu O, Dua K, Chellappan DK, Katavic PL, Collet TA. Recent update on anti-dengue drug discovery. Eur J Med Chem 2019; 176: 431-55.
[http://dx.doi.org/10.1016/j.ejmech.2019.05.010] [PMID: 31128447]
[8]
Kumavat SD, Chaudhari YS, Borole P, Mishra P, Shenghani K, Duvvuri P. Degradation studies of curcumin. Int J Pharm Rev Res 2013; 3(2): 50-5.
[9]
Padilla-S L, Rodríguez A, Gonzales MM, et al. Inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro. Arch Virol 2014; 159(3): 573-9.
[http://dx.doi.org/10.1007/s00705-013-1849-6] [PMID: 24081825]
[10]
Balasubramanian A, Pilankatta R, Teramoto T, et al. Inhibition of dengue virus by curcuminoids. Antiviral Res 2019; 162: 71-8.
[http://dx.doi.org/10.1016/j.antiviral.2018.12.002] [PMID: 30529358]
[11]
Glickman MH, Ciechanover A. The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol Rev 2002; 82(2): 373-428.
[http://dx.doi.org/10.1152/physrev.00027.2001] [PMID: 11917093]
[12]
Chen TY, Chen DY, Wen HW, et al. Inhibition of enveloped viruses infectivity by curcumin. PLoS One 2013; 8(5): e62482.
[http://dx.doi.org/10.1371/journal.pone.0062482] [PMID: 23658730]
[13]
Wang JL, Zhang JL, Chen W, et al. Roles of small GTPase Rac1 in the regulation of actin cytoskeleton during dengue virus infection. PLoS Negl Trop Dis 2010; 4(8): e809.
[http://dx.doi.org/10.1371/journal.pntd.0000809] [PMID: 20824170]
[14]
Qin Y, Lin L, Chen Y, et al. Curcumin inhibits the replication of enterovirus 71 in vitro. Acta Pharm Sin B 2014; 4(4): 284-94.
[http://dx.doi.org/10.1016/j.apsb.2014.06.006] [PMID: 26579397]
[15]
Rachmawati H, Soraya IS, Kurniati NF, Rahma A. In vitro study on antihypertensive and antihypercholesterolemic effects of a curcumin nanoemulsion. Sci Pharm 2016; 84(1): 131-40.
[http://dx.doi.org/10.3797/scipharm.ISP.2015.05] [PMID: 27110504]
[16]
Nabila N, Suada NK, Denis D, et al. Antiviral action of curcumin encapsulated in nanoemulsion against four serotypes of dengue virus. Pharm Nanotechnol 2020; 8(1): 54-62.
[http://dx.doi.org/10.2174/2211738507666191210163408] [PMID: 31858909]
[17]
Rachmawati H, Budiputra DK, Mauludin R. Curcumin nanoemulsion for transdermal application: formulation and evaluation. Drug Dev Ind Pharm 2015; 41(4): 560-6.
[http://dx.doi.org/10.3109/03639045.2014.884127] [PMID: 24502271]
[18]
Wei X, Shao B, He Z, et al. Cationic nanocarriers induce cell necrosis through impairment of Na(+)/K(+)-ATPase and cause subsequent inflammatory response. Cell Res 2015; 25(2): 237-53.
[http://dx.doi.org/10.1038/cr.2015.9] [PMID: 25613571]
[19]
Forest V, Pourchez J. Preferential binding of positive nanoparticles on cell membranes is due to electrostatic interactions: A too simplistic explanation that does not take into account the nanoparticle protein corona. Mater Sci Eng C 2017; 70(Pt 1): 889-96.
[http://dx.doi.org/10.1016/j.msec.2016.09.016] [PMID: 27770966]
[20]
Farshbaf M, Davaran S, Zarebkohan A, Annabi N, Akbarzadeh A, Salehi R. Significant role of cationic polymers in drug delivery systems. Artif Cells Nanomed Biotechnol 2018; 46(8): 1872-91.
[PMID: 29103306]
[21]
Du XJ, Wang JL, Iqbal S, et al. The effect of surface charge on oral absorption of polymeric nanoparticles. Biomater Sci 2018; 6(3): 642-50.
[http://dx.doi.org/10.1039/C7BM01096F] [PMID: 29412203]
[22]
Osaka T, Nakanishi T, Shanmugam S, Takahama S, Zhang H. Effect of surface charge of magnetite nanoparticles on their internalization into breast cancer and umbilical vein endothelial cells. Colloids Surf B Biointerfaces 2009; 71(2): 325-30.
[http://dx.doi.org/10.1016/j.colsurfb.2009.03.004] [PMID: 19361963]
[23]
Abbaszadegan A, Ghahramani Y, Gholami A, et al. The effect of charge at the surface of silver nanoparticles on antimicrobial activity against gram-positive and gram-negative bacteria : a preliminary study. J Nanomater 2015; 2015: 720654.
[http://dx.doi.org/10.1155/2015/720654]
[24]
Hu Y, Sun H, Hu N. Assembly of layer-by-layer films of electroactive hemoglobin and surfactant didodecyldimethylammonium bromide. J Colloid Interface Sci 2007; 314(1): 131-40.
[http://dx.doi.org/10.1016/j.jcis.2007.05.057] [PMID: 17585929]
[25]
Zhao T, Chen H, Yang L, et al. DDAB-modified TPGS-b-(PCL-ran-PGA) nnoparticles as oral anticancer drug carrier for lung cancer chemotherapy. World Scientific Publishing Company 2013; 8(2): 1350014.
[26]
Song C, Labhasetwar V, Cui X, Underwood T, Levy RJ. Arterial uptake of biodegradable nanoparticles for intravascular local drug delivery: results with an acute dog model. J Control Release 1998; 54(2): 201-11.
[http://dx.doi.org/10.1016/S0168-3659(98)00016-9] [PMID: 9724907]
[27]
Madishetty S, Syed MA, Kandadi P, Veerabrahma K. Cationic Diclofenac Lipid Nanoemulsion for Improved Oral Bioavailability : preparation, characterization and in vivo evaluation. Int J Pharm Sci Nanotech 2015; 8(2): 2874-80.
[28]
Wu L, Zhang J, Watanabe W. Physical and chemical stability of drug nanoparticles. Adv Drug Deliv Rev 2011; 63(6): 456-69.
[http://dx.doi.org/10.1016/j.addr.2011.02.001] [PMID: 21315781]
[29]
Moghadamtousi SZ, Kadir HA, Hassandarvish P, Tajik H, Abubakar S, Zandi K. A review on antibacterial, antiviral, and antifungal activity of curcumin. BioMed Res Int 2014; 2014: 186864.
[PMID: 24877064]
[30]
Kusumoto K, Ishikawa T. Didodecyldimethylammonium bromide (DDAB) induces caspase-mediated apoptosis in human leukemia HL-60 cells. J Control Release 2010; 147(2): 246-52.
[http://dx.doi.org/10.1016/j.jconrel.2010.07.114] [PMID: 20674638]
[31]
Yao Q, Lin M, Wang Y, et al. Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways. Int J Mol Med 2015; 36(4): 1118-26.
[http://dx.doi.org/10.3892/ijmm.2015.2327] [PMID: 26310655]
[32]
Chen Q, Lu G, Wang Y, et al. Cytoskeleton disorganization during apoptosis induced by curcumin in A549 lung adenocarcinoma cells. Planta Med 2009; 75(8): 808-13.
[http://dx.doi.org/10.1055/s-0029-1185399] [PMID: 19266427]
[33]
Silva AM, Gomes CM, Coutinho TE, et al. Soft cationic nanoparticles for drug delivery: production and cytotoxicity of solid lipid nanoparticles (SLNs). Appl Sci (Basel) 2019; 2019(9): 4438.
[http://dx.doi.org/10.3390/app9204438]

Rights & Permissions Print Cite
Article Metrics
15
1
© 2024 Bentham Science Publishers | Privacy Policy