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The Natural Products Journal

Editor-in-Chief

ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

Research Article

Echinochrome Pigment Improves Male Rats' Fertility

Author(s): Neveen Asmet Farag, Ayman S Mohamed*, Hanan Farag El Sayed, Eman Y. Salah EL Din and Abdel Rahman A. Tawfik

Volume 12, Issue 3, 2022

Published on: 22 December, 2021

Article ID: e160921188044 Pages: 7

DOI: 10.2174/2210315510999201116205519

Price: $65

Abstract

Background: Infertility is the first-rate public health problem affecting one in five married couples globally; male causes embody a significant proportion. Natural products could be an alternative or complementary inexpensive treatment for such matters. Echinochrome (Ech) is a natural quinone pigment obtained from sea urchin, and it was confirmed to possess many pharmacological properties due to its chemical activity.

Objective: The current research paper was targeted to evaluate the potential effects of Ech on male fertility, and to highlight the possible involved mechanisms.

Methods: Eighteen adult male rats were randomly distributed into three groups: control (1 ml of 2% DMSO, p.o.), low dose Ech (0.1 mg/kg, p.o.), and high dose Ech (1 mg/kg p.o.).

Results: The high dose Ech caused a significant decline in the levels of glucose, ALT, AST, ALP, urea, Cr, uric acid, TG, TC and LDL-C and testicular tissue MDA, while it caused a significant rise in the levels of albumin, TP, HDL-C, FSH, LH, testosterone and testicular tissue GSH activity. Moreover, it showed a significant positive effect on the testis weight, caudal epididymis weight, sperm count, sperm motility, sperm morphology, fructose concentration, and α-glucosidase activity. However, no significant changes were observed in the histological examination of testicular tissue among all groups.

Conclusion: High dose Ech improved male rat-fertility either directly by activating the pituitarygonadal axis, and or indirectly via enhancing the renal and hepatic functions, the lipid profile and or the antioxidant pathways.

Keywords: Echinochrome, sea urchin, infertility, testis, oxidative stress, male fertility.

Graphical Abstract

[1]
Brugo-Olmedo, S.; Chillik, C.; Kopelman, S. Definition and causes of infertility. Reprod. Biomed. Online, 2001, 2(1), 41-53.
[http://dx.doi.org/10.1016/S1472-6483(10)62187-6] [PMID: 12537824]
[2]
Isidori, A.M.; Pozza, C.; Gianfrilli, D.; Isidori, A. Medical treatment to improve sperm quality. Reprod. Biomed. Online, 2006, 12(6), 704-714.
[http://dx.doi.org/10.1016/S1472-6483(10)61082-6] [PMID: 16792845]
[3]
Agarwal, A.; Mulgund, A.; Hamada, A.; Chyatte, M.R. A unique view on male infertility around the globe. Reprod. Biol. Endocrinol., 2015, 13, 37.
[http://dx.doi.org/10.1186/s12958-015-0032-1] [PMID: 25928197]
[4]
Thoma, M.E.; McLain, A.C.; Louis, J.F.; King, R.B.; Trumble, A.C.; Sundaram, R.; Buck Louis, G.M. Prevalence of infertility in the United States as estimated by the current duration approach and a traditional constructed approach. Fertil. Steril., 2013, 99(5), 1324-1331.e1.
[http://dx.doi.org/10.1016/j.fertnstert.2012.11.037] [PMID: 23290741]
[5]
Smith, R.; Kaune, H.; Parodi, D.; Madariaga, M.; Rios, R.; Morales, I.; Castro, A. Increased sperm DNA damage in patients with varicocele: Relationship with seminal oxidative stress. Hum. Reprod., 2006, 21(4), 986-993.
[http://dx.doi.org/10.1093/humrep/dei429] [PMID: 16361286]
[6]
Agarwal, A.; Gupta, S.; Sikka, S. The role of free radicals and antioxidants in reproduction. Curr. Opin. Obstet. Gynecol., 2006, 18(3), 325-332.
[http://dx.doi.org/10.1097/01.gco.0000193003.58158.4e] [PMID: 16735834]
[7]
Agarwal, A.; Nallella, K.P.; Allamaneni, S.S.; Said, T.M. Role of antioxidants in treatment of male infertility: An overview of the literature. Reprod. Biomed. Online, 2004, 8(6), 616-627.
[http://dx.doi.org/10.1016/S1472-6483(10)61641-0] [PMID: 15169573]
[8]
Cemile Merv, S. Çiğdem Elmas. The effects of oxidative stress and some of the popular antioxidants on reproductive system: A mini review. J. Nutr. Food Sci., 2016, 6(2), 1000464.
[9]
Smits, R.M.; Mackenzie-Proctor, R.; Fleischer, K.; Showell, M.G. Antioxidants in fertility: Impact on male and female reproductive outcomes. Fertil. Steril., 2018, 110(4), 578-580.
[http://dx.doi.org/10.1016/j.fertnstert.2018.05.028] [PMID: 30196940]
[10]
Fahmy, S.R.; Mohamed, A.S. Holoturia arenicola extract modulates bile duct ligation-induced oxidative stress in rat kidney. Int. J. Clin. Exp. Pathol., 2015, 8(2), 1649-1657.
[PMID: 25973050]
[11]
Mayer, A.M.S.; Rodríguez, A.D.; Taglialatela-Scafati, O.; Fusetani, N. Marine pharmacology in 2009-2011: Marine compounds with antibacterial, antidiabetic, antifungal, anti-inflammatory, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous systems, and other miscellaneous mechanisms of action. Mar. Drugs, 2013, 11(7), 2510-2573.
[http://dx.doi.org/10.3390/md11072510] [PMID: 23880931]
[12]
Lang, C.; Schroeter, S.C. Change in sea urchin populations after the destruction of kelp beds. Mar. Biol., 1976, 36, 321-326.
[http://dx.doi.org/10.1007/BF00389193]
[13]
Mohamed, A.S. M SA, Marie MAS. The possible hypoglycemic mechanisms of echinochrome. Curr. Diabetes Rev., 2017, 14(4), 13.
[14]
Mohamed, A.S. Echinochrome exhibits antitumor activity against ehrlich ascites carcinoma in swiss albino mice. Nutr. Cancer., 2020, 2020, 1-9.
[http://dx.doi.org/10.1080/01635581.2020.1737152] [PMID: 32151164]
[15]
Amarowicz, R.; Synowiecki, J.; Shahidi, F. Sephadex LH-20 separation of pigments from shells of red sea urchin (Strongylocentrotus franciscanus). Food Chem., 1994, 51, 227-229.
[http://dx.doi.org/10.1016/0308-8146(94)90262-3]
[16]
Kuwahara, R.; Hatatea, H.; Yukia, T.; Murata, H.; Tanakac, R.; Hamad, Y. Antioxidant property of polyhydroxylated naphthoquinone pigments from shells of purple sea urchin Anthocidaris crassispina. Lebensm. Wiss. Technol., 2009, 42, 1296-1300.
[http://dx.doi.org/10.1016/j.lwt.2009.02.020]
[17]
Solomon, M.C.; Erasmus, N.; Henkel, R.R. In vivo effects of Eurycoma longifolia Jack (Tongkat Ali) extract on reproductive functions in the rat. Andrologia, 2014, 46(4), 339-348.
[http://dx.doi.org/10.1111/and.12082] [PMID: 23464350]
[18]
Miyamoto, T.; Tsujimura, A.; Miyagawa, Y.; Koh, E.; Namiki, M.; Sengoku, K. Male infertility and its causes in human. Adv. Urol., 2012, 2012, 384520.
[http://dx.doi.org/10.1155/2012/384520] [PMID: 22046184]
[19]
Fahmy, S.R.; Zaki, N.I.; Eid, S.Z.; Mohamed, A.S.; Hassanein, S.S. Effectiveness of echinochrome on HFD-Induced hyperlipidemia in rats. Nat. Prod. Bioprospect., 2019, 9(5), 337-344.
[http://dx.doi.org/10.1007/s13659-019-00221-4] [PMID: 31628663]
[20]
Saez Lancellotti, T.E.; Boarelli, P.V.; Monclus, M.A.; Cabrillana, M.E.; Clementi, M.A.; Espínola, L.S.; Cid Barría, J.L.; Vincenti, A.E.; Santi, A.G.; Fornés, M.W. Hypercholesterolemia impaired sperm functionality in rabbits. PLoS One, 2010, 5(10), e13457.
[http://dx.doi.org/10.1371/journal.pone.0013457] [PMID: 20976152]
[21]
Jarvis, S.; Williamson, C.; Bevan, C.L. Liver X receptors and male (In)fertility. Int. J. Mol. Sci., 2019, 20(21), 11-14.
[http://dx.doi.org/10.3390/ijms20215379] [PMID: 31671745]
[22]
Charni-Natan, M.; Aloni-Grinstein, R.; Osher, E.; Rotter, V. Liver and steroid hormones-can a touch of p53 make a difference? Front. Endocrinol. (Lausanne), 2019, 10, 374.
[http://dx.doi.org/10.3389/fendo.2019.00374] [PMID: 31244779]
[23]
Ramachandran, A.; Jaeschke, H. Oxidative stress and acute hepatic injury. Curr. Opin. Toxicol., 2018, 7, 17-21.
[http://dx.doi.org/10.1016/j.cotox.2017.10.011] [PMID: 29399645]
[24]
Yamada, J.; Tomiyama, H.; Yambe, M.; Koji, Y.; Motobe, K.; Shiina, K.; Yamamoto, Y.; Yamashina, A. Elevated serum levels of alanine aminotransferase and gamma glutamyltransferase are markers of inflammation and oxidative stress independent of the metabolic syndrome. Atherosclerosis, 2006, 189(1), 198-205.
[http://dx.doi.org/10.1016/j.atherosclerosis.2005.11.036] [PMID: 16405892]
[25]
Maresch, C.C.; Stute, D.C.; Fleming, T.; Lin, J.; Hammes, H.P.; Linn, T. Hyperglycemia induces spermatogenic disruption via major pathways of diabetes pathogenesis. Sci. Rep., 2019, 9(1), 13074.
[http://dx.doi.org/10.1038/s41598-019-49600-4] [PMID: 31506549]
[26]
Mohamed, A.S.; Soliman, A.M.; Marie, M.A.S. Mechanisms of echinochrome potency in modulating diabetic complications in liver. Life Sci., 2016, 151, 41-49.
[http://dx.doi.org/10.1016/j.lfs.2016.03.007] [PMID: 26947587]
[27]
Soliman, AM Potential impact of Paracentrotus lividus extract on diabetic rat models induced by lividus Paracentrotus Potential impact of high fat diet/streptozotocin. J B A Z., 2016, 77, 8-20.
[28]
Ndisang, J.F. Role of heme oxygenase in inflammation, insulin-signalling, diabetes and obesity. Mediators Inflamm., 2010, 2010, 359732.
[http://dx.doi.org/10.1155/2010/359732] [PMID: 20508722]
[29]
Schmidt, A.; Luger, A.; Hörl, W.H. Sexual hormone abnormalities in male patients with renal failure. Nephrol. Dial. Transplant., 2002, 17(3), 368-371.
[http://dx.doi.org/10.1093/ndt/17.3.368] [PMID: 11865078]
[30]
Agarwal, A.; Virk, G.; Ong, C.; du Plessis, S.S. Effect of oxidative stress on male reproduction. World J. Mens Health, 2014, 32(1), 1-17.
[http://dx.doi.org/10.5534/wjmh.2014.32.1.1] [PMID: 24872947]
[31]
Appasamy, M.; Muttukrishna, S.; Pizzey, A.R.; Ozturk, O.; Groome, N.P.; Serhal, P.; Jauniaux, E. Relationship between male reproductive hormones, sperm DNA damage and markers of oxidative stress in infertility. Reprod. Biomed. Online, 2007, 14(2), 159-165.
[http://dx.doi.org/10.1016/S1472-6483(10)60783-3] [PMID: 17298717]
[32]
Martin-Hidalgo, D.; Bragado, M.J.; Batista, A.R.; Oliveira, P.F.; Alves, M.G. Antioxidants and male fertility: From molecular studies to clinical evidence. Antioxidants, 2019, 8(4), 89.
[http://dx.doi.org/10.3390/antiox8040089] [PMID: 30959797]
[33]
Kubota, H.; Brinster, R.L. Spermatogonial stem cells. Biol. Reprod., 2018, 99(1), 52-74.
[http://dx.doi.org/10.1093/biolre/ioy077] [PMID: 29617903]
[34]
Martini, A.C.; Molina, R.I.; Vincenti, L.M.; Santillán, M.E.; Stutz, G.; Ruiz, R.D.; Fiol de Cuneo, M. Neutral α-glucosidase activity in mouse: A marker of epididymal function? Reprod. Fertil. Dev., 2007, 19(4), 563-568.
[http://dx.doi.org/10.1071/RD06070] [PMID: 17524301]
[35]
Mann, T.; Lutwak-Mann, C. Male reproductive function and the composition of semen: General considerations. Male Reproductive Function and Semen London; Springer, 1981.
[http://dx.doi.org/10.1007/978-1-4471-1300-3_1]
[36]
Gonzales, G.F.; Kortebani, G.; Mazzolli, A.B. Hyperviscosity and hypofunction of the seminal vesicles. Arch. Androl., 1993, 30(1), 63-68.
[http://dx.doi.org/10.3109/01485019308988370] [PMID: 8420506]

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