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Current Genomics

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ISSN (Print): 1389-2029
ISSN (Online): 1875-5488

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Letter Article

Discrepant mRNA and Protein Expression in Immune Cells

Author(s): Jiawei Li, Yi Zhang, Cheng Yang* and Ruiming Rong*

Volume 21, Issue 8, 2020

Page: [560 - 563] Pages: 4

DOI: 10.2174/1389202921999200716103758

Price: $65

Abstract

With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted to identify new methods for performing in-depth studies of immune cells. However, the discrepancies between the mRNA levels and the levels of surface proteins have confused many researchers. Here, we report a significant and interesting phenomenon in which the mRNA and protein expression levels were mismatched in immune cells. We concluded that scRNA-seq should be combined with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of both mRNA and protein expression will enhance the precision and credibility of the results.

Keywords: Single-cell mRNA sequencing, immune cell, mRNA, protein, myeloid-derived cells, protein expression.

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[1]
Macosko, E.Z.; Basu, A.; Satija, R.; Nemesh, J.; Shekhar, K.; Goldman, M.; Tirosh, I.; Bialas, A.R.; Kamitaki, N.; Martersteck, E.M.; Trombetta, J.J.; Weitz, D.A.; Sanes, J.R.; Shalek, A.K.; Regev, A.; McCarroll, S.A. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell, 2015, 161(5), 1202-1214.
[http://dx.doi.org/10.1016/j.cell.2015.05.002] [PMID: 26000488]
[2]
Zheng, G.X.; Terry, J.M.; Belgrader, P.; Ryvkin, P.; Bent, Z.W.; Wilson, R.; Ziraldo, S.B.; Wheeler, T.D.; McDermott, G.P.; Zhu, J.; Gregory, M.T.; Shuga, J.; Montesclaros, L.; Underwood, J.G.; Masquelier, D.A.; Nishimura, S.Y.; Schnall-Levin, M.; Wyatt, P.W.; Hindson, C.M.; Bharadwaj, R.; Wong, A.; Ness, K.D.; Beppu, L.W.; Deeg, H.J.; McFarland, C.; Loeb, K.R.; Valente, W.J.; Ericson, N.G.; Stevens, E.A.; Radich, J.P.; Mikkelsen, T.S.; Hindson, B.J.; Bielas, J.H. Massively parallel digital transcriptional profiling of single cells. Nat. Commun., 2017, 8, 14049.
[http://dx.doi.org/10.1038/ncomms14049] [PMID: 28091601]
[3]
Liu, Y.; Beyer, A.; Aebersold, R. On the Dependency of Cellular Protein Levels on mRNA Abundance. Cell, 2016, 165(3), 535-550.
[http://dx.doi.org/10.1016/j.cell.2016.03.014] [PMID: 27104977]
[4]
Gabrilovich, D.I. Myeloid-derived suppressor cells. Cancer Immunol. Res., 2017, 5(1), 3-8.
[http://dx.doi.org/10.1158/2326-6066.CIR-16-0297] [PMID: 28052991]
[5]
Veglia, F.; Perego, M.; Gabrilovich, D. Myeloid-derived suppressor cells coming of age. Nat. Immunol., 2018, 19(2), 108-119.
[http://dx.doi.org/10.1038/s41590-017-0022-x] [PMID: 29348500]
[6]
Peterson, V.M.; Zhang, K.X.; Kumar, N.; Wong, J.; Li, L.; Wilson, D.C.; Moore, R.; McClanahan, T.K.; Sadekova, S.; Klappenbach, J.A. Multiplexed quantification of proteins and transcripts in single cells. Nat. Biotechnol., 2017, 35(10), 936-939.
[http://dx.doi.org/10.1038/nbt.3973] [PMID: 28854175]
[7]
Ziegenhain, C.; Vieth, B.; Parekh, S.; Reinius, B.; Guillaumet-Adkins, A.; Smets, M. Comparative analysis of single-cell RNA sequencing methods. Mol. Cell, 2017, 65(4), 631-643.e4.
[http://dx.doi.org/10.1016/j.molcel.2017.01.023]
[8]
Mair, F.; Erickson, J.R.; Voillet, V.; Simoni, Y.; Bi, T.; Tyznik, A.J.; Martin, J.; Gottardo, R.; Newell, E.W.; Prlic, M. A targeted multi-omic analysis approach measures protein expression and low-abundance transcripts on the single-cell level. Cell Rep., 2020, 31(1)107499
[http://dx.doi.org/10.1016/j.celrep.2020.03.063] [PMID: 32268080]
[9]
Corridoni, D.; Chapman, T.; Antanaviciute, A.; Satsangi, J.; Simmons, A. Inflammatory bowel disease through the lens of single-cell rna-seq technologies. Inflamm. Bowel Dis., 2020, 26(11), 1658-1668.
[http://dx.doi.org/10.1093/ibd/izaa089] [PMID: 32386055]
[10]
Saigusa, R.; Ley, K. CITE-Seq hits vascular medicine. Clin. Chem., 2020, 66(6), 751-753.
[http://dx.doi.org/10.1093/clinchem/hvaa016] [PMID: 32091084]

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