Login Register Cart 0
Generic placeholder image

Current Pharmaceutical Analysis

Editor-in-Chief

ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Back
Research Article

Development of Reverse Phase Ultra-fast Liquid Chromatography Using Ion-pairing Reagent for Quantitative Assessment of Ceftriaxone in Rat Serum and Cerebrospinal Fluid

Author(s): Swarupanjali Padhi*, Rupa Mazumder and Shradha Bisth

Volume 17, Issue 8, 2021

Published on: 26 May, 2020

Page: [1088 - 1092] Pages: 5

DOI: 10.2174/1573412916999200526112921

Price: $65

Abstract

Background: In case of meningitis, the meninges are inflamed and the blood brain barrier is distorted, therefore, there is no hindrance to drug penetration. The problem arises when the disease is at the verge of cure, the meninges become uninflamed and the permeability of the drug is reduced to such extend that it becomes nearly impossible to maintain the minimum inhibitory concentration of drug at the site of infection. This problem was overcome by formulating ceftriaxone loaded NLCs and administrating it through intraperitoneal route to Wistar Albino rat. For quantitative assessment of drugs in rat serum and cerebrospinal spinal fluid, a new RP-UFLC (reverse-phase ultra-fast liquid chromatography) method has been developed and validated.

Objective: Development and validation of RP-UFLC (using ion-pairing reagent) method for accurate estimation of ceftriaxone in rat serum and CSF.

Methods: Method validation is done according to the ICH Guidelines (Q2) for the estimation of ceftriaxone in rat serum and its CSF (cerebrospinal fluid) by the RP-UFLC method. The blood was collected from the rat tail vein and CSF was collected carefully from cisterna magna of the rats by a 23G syringe. The mobile phase was used in a ratio of 70:30%v/v of phosphate buffer with ion-pairing reagent and acetonitrile with a pH of 8.0

Results: Limit of detection and limit of quantification of ceftriaxone in rat serum was 1.08 μg/ml and 3.84 μg/ml, respectively. Similarly, the limit of detection and limit of quantification of ceftriaxone in CSF of rats was 0.94 μg/ml and 2.84 μg/ml, respectively. In both cases, the R2 value was more than 0.99 and showed 99% accuracy.

Conclusion: The experimental results suggest that the new RP-UFLC method developed and validated can be effectively used to assess ceftriaxone in preclinical studies in rats.

Keywords: Ion-pairing reagent, limit of detection, limit of quantification, recovery data, ceftriaxone, Streptococcus pneumoniae.

« Previous Next »
Graphical Abstract

[1]
Nau, R.; Prange, H.W.; Muth, P.; Mahr, G.; Menck, S.; Kolenda, H.; Sörgel, F. Passage of cefotaxime and ceftriaxone into cerebrospinal fluid of patients with uninflamed meninges. Antimicrob. Agents Chemother., 1993, 37(7), 1518-1524.
[http://dx.doi.org/10.1128/AAC.37.7.1518] [PMID: 8363385]
[2]
Latif, R.; Dajani, A.S. Ceftriaxone diffusion into cerebrospinal fluid of children with meningitis. Antimicrob. Agents Chemother., 1983, 23(1), 46-48.
[http://dx.doi.org/10.1128/AAC.23.1.46] [PMID: 6299184]
[3]
Lee, S.O.; Lee, E.S.; Park, S.Y.; Kim, S.Y.; Seo, Y.H.; Cho, Y.K. Reduced use of third-generation cephalosporins decreases the acquisition of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae. Infect. Control Hosp. Epidemiol., 2004, 25(10), 832-837.
[http://dx.doi.org/10.1086/502304] [PMID: 15518024]
[4]
Vinodkumar, C.S.; Neelagund, Y.F. Emergence of extended spectrum beta lactamase mediated resistance in neonatal septicemia. Indian J. Pathol. Microbiol., 2006, 49(4), 616-619.
[PMID: 17183878]
[5]
Paterson, D.L.; Bonomo, R.A. Extended-spectrum beta-lactamases: a clinical update. Clin. Microbiol. Rev., 2005, 18(4), 657-686.
[http://dx.doi.org/10.1128/CMR.18.4.657-686.2005] [PMID: 16223952]
[6]
Emmerson, A.M.; Lamport, P.A.; Reeves, D.S.; Bywater, M.J.; Holt, H.A.R.; Wise, R.; Andrews, J.; Hall, M.J. The in vitro antibacterial activity of ceftriaxone in comparison with nine other antibiotics. Curr. Med. Res. Opin., 1985, 9(7), 480-493.
[http://dx.doi.org/10.1185/03007998509109622] [PMID: 3928265]
[7]
Panda, S.S.; Kumar, B.R.; Patel, P.K.; Panda, J. New RP-UFLC Method for Estimation of Xanthinol Nicotinate in Pharmaceutical Dosage Form. Chem. Sci. Trans., 2013, 2, 656-662.
[http://dx.doi.org/10.7598/cst2013.379]
[8]
Panda, S.S.; Sharma, K.; Mohanty, B.; Bera, R.K.V.V.; Acharjya, S.K.; Beg, S. Integrated quality by design (QbD) and design of experiments (DoE) approach for UFLC determination of telaprevir in rat serum. J. Liq. Chromatogr. Relat. Technol., 2017, 40, 951-958.
[http://dx.doi.org/10.1080/10826076.2017.1387558]
[9]
Gumustas, M.; Kurbanoglu, S.; Uslu, B. UPLC versus HPLC on Drug Analysis: Advantageous, Applications and Their Validation Parameters. Chromatographia, 2013, 76, 1365-1427.
[http://dx.doi.org/10.1007/s10337-013-2477-8]
[10]
Pal, N.; Rao, A.S.; Hadi, M.A. HPLC method development and validation for the assay of ceftriaxone sodium injection. Int. J. Pharma Sci., 2012, 2(4), 84-90.
[11]
Sultana, N.; Orayne, M.S.; Shahzad, W. Simultaneous determination of ceftriaxone sodium and Statin Drugs in Pharmaceutical formulations and Human Serum by RP-HPLC. J. Chil. Chem. Soc., 2010, 55(2), 193-198.
[http://dx.doi.org/10.4067/S0717-97072010000200010]
[12]
Granich, G.G.; Krogstad, D.J. Ion pair high-performance liquid chromatographic assay for ceftriaxone. Antimicrob. Agents Chemother., 1987, 31(3), 385-388.
[http://dx.doi.org/10.1128/AAC.31.3.385] [PMID: 3579254]
[13]
Cecchi, T.; Passamonti, P. Retention mechanism for ion-pair chromatography with chaotropic reagents. J. Chromatogr. A, 2009, 1216(10), 1789-1797.
[http://dx.doi.org/10.1016/j.chroma.2008.10.031] [PMID: 18973902]
[14]
John, H.; Knox, R.; Hartwick, A. Mechanism of ion-pair liquid chromatography of amines, neutrals, zwitterions and acids using anionic hetaerons. J. Chromatogr. A, 1981, 304, 3-21.
[15]
Vailaya, A.; Horváth, C. Retention in reversed-phase chromatography: partition or adsorption? J. Chromatogr. A, 1998, 829(1-2), 1-27.
[http://dx.doi.org/10.1016/S0021-9673(98)00727-4] [PMID: 9923077]
[16]
Nirogi, R.; Kandikere, V.; Mudigonda, K.; Bhyrapuneni, G.; Muddana, N.; Saralaya, R.; Benade, V. A simple and rapid method to collect the cerebrospinal fluid of rats and its application for the assessment of drug penetration into the central nervous system. J. Neurosci. Methods, 2009, 178(1), 116-119.
[http://dx.doi.org/10.1016/j.jneumeth.2008.12.001] [PMID: 19109998]
[17]
Satveer, J.S.; Jalalpure, S.; Dhamecha, D.; Hua, G. S.; Jadhav, K. Development and Validation of Reverse-Phase High-Performance Liquid Chromatographic Method for Determination of Resveratrol in Human and Rat Plasma for Preclinical and Clinical Studies. Indian J of Pharmaceutical Education and Research., 2020, 54(1), 187-193.
[18]
Łabuzek, K.; Suchy, D.; Gabryel, B.; Bielecka, A.; Liber, S.; Okopień, B. Quantification of metformin by the HPLC method in brain regions, cerebrospinal fluid and plasma of rats treated with lipopolysaccharide. Pharmacol. Rep., 2010, 62(5), 956-965.
[http://dx.doi.org/10.1016/S1734-1140(10)70357-1] [PMID: 21098880]
[19]
Gomes, M.L.S.; Klein, T.; Simionatto, M.; Nadal, J.M.; Zanin, S.M.W.; Borsato, D.M.; Farago, P.V. A simple RP-HPLC/UV method for determination of cilostazol in polymeric nanoparticles suspensions: development and validation. Lat. Am. J. Pharm., 2015, 34(4), 803-809.
[20]
Clarissa, E.L.; Gisele, L.; Traudi, K.; Priscileila, C.F.; Paulo, V.F. A simple HPLC method for the determination of halcinonide in lipid nanoparticles: development, validation, encapsulation efficiency, and in vitro drug permeation. Braz. J. Pharm. Sci., 2017, 53(2)

Rights & Permissions Print Cite
Article Metrics
8
1
© 2024 Bentham Science Publishers | Privacy Policy