Abstract
Objective: Pioglitazone (PG) is used to control high blood sugar in patients with type 2 diabetes mellitus. PG acts as a peroxisome proliferator-activated receptor γ agonist. In addition to the insulin-sensitizing effect, PG possesses anti-inflammatory effect. In this study, the protective effect of PG was evaluated against DNA damage induced by ionizing radiation in healthy human lymphocytes.
Methods: The microtubes containing human whole blood were treated with PG at various concentrations (1-50 μM) for three hours. Then, the blood samples were irradiated with X-ray. Lymphocytes were cultured for determining the frequency of micronuclei as a genotoxicity biomarker in binucleated lymphocytes.
Results: The mean percentage of micronuclei was significantly increased in human lymphocytes when exposed to IR, while it was decreased in lymphocytes pre-treated with PG. The maximum reduction in the frequency of micronuclei in irradiated lymphocytes was observed at 5 μM of PG treatment (48% decrease).
Conclusion: The anti-inflammatory property suggested the mechanism action of PG for protecting human lymphocytes against genotoxicity induced by ionizing radiation.
Keywords: Anti-inflammatory, DNA damage, micronucleus test, pioglitazone, radiation biology, radioprotective.
Graphical Abstract
[http://dx.doi.org/10.1016/j.drudis.2007.07.017] [PMID: 17933679]
[http://dx.doi.org/10.1016/j.drudis.2016.02.019] [PMID: 26955911]
[http://dx.doi.org/10.3389/fphar.2018.01093] [PMID: 30337873]
[http://dx.doi.org/10.1016/j.ejphar.2015.11.004] [PMID: 26632493]
[http://dx.doi.org/10.1016/j.dsx.2014.09.015] [PMID: 25450819]
[http://dx.doi.org/10.1016/j.bbi.2017.02.001] [PMID: 28167117]
[http://dx.doi.org/10.3803/EnM.2018.33.1.105] [PMID: 29589392]
[http://dx.doi.org/10.1016/j.jss.2015.02.007] [PMID: 25772146]
[http://dx.doi.org/10.1016/j.tice.2015.01.003] [PMID: 25712322]
[http://dx.doi.org/10.1186/s12929-015-0187-2] [PMID: 26394827]
[http://dx.doi.org/10.1016/j.intimp.2018.06.037] [PMID: 29966944]
[PMID: 17214065]
[http://dx.doi.org/10.4103/1735-5362.199051] [PMID: 28255318]
[http://dx.doi.org/10.3857/roj.2015.33.3.256] [PMID: 26484310]
[http://dx.doi.org/10.14744/etd.2019.35762]
[http://dx.doi.org/10.3390/nu9121371] [PMID: 29258247]
[http://dx.doi.org/10.12659/MSM.913461] [PMID: 30820023]
[http://dx.doi.org/10.1007/s10753-018-0851-2] [PMID: 30022363]
[http://dx.doi.org/10.1007/s10495-016-1280-z] [PMID: 27515513]
[http://dx.doi.org/10.2174/0929867323666160824162718 ] [PMID: 27554808]