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Current Pharmaceutical Design

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ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

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General Research Article

A Peptide Construct Mediates Focal Adhesion Pathway Through the Activation of Integrin Receptor

Author(s): Mohsen Khosravi, Naser Kakavandi, Shima Rezaee, Mohammad Shabani* and Mohammad Najafi*

Volume 26, Issue 15, 2020

Page: [1749 - 1755] Pages: 7

DOI: 10.2174/1381612826666200311125325

Price: $65

Abstract

Background: The integrin family receptors stimulate the cellular proliferation and migration through the focal adhesion pathway by the activation of PTK2, VASP and TSP1 proteins. The purpose of this study was to investigate the integrin-ligated motifs through the activation of focal adhesion pathway.

Methods: A chimeric peptide was predicted from the integrin-mediated ligands by bioinformatics tools. The VSMCs were treated with the chimeric peptide and simvastatin. The PTK2, VASP and TSP1 protein and gene expression levels were measured by RT-qPCR and Western Blotting techniques, respectively. AutoDock Tools were used for the docking technique.

Results: The PTK2, VASP and TSP1 protein expression levels increased significantly in the VSMCs treated with chimeric peptide in conversely with the effects of simvastatin. The docking results suggested two motifs in the chimeric peptide.

Conclusion: In conclusion, the chimeric peptide activated the focal adhesion pathway. The motifs 1 and 2 may be directly involved in the transduction of signal by integrin family receptors.

Keywords: Peptide, integrin, focal adhesion, PKT2, VASP, TSP1, western blotting.

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