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Current Chromatography

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ISSN (Print): 2213-2406
ISSN (Online): 2213-2414

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Research Article

Distribution of Quercetin in Different Organs of Male Sprague Dawley Rats Under Hypoxia: A High-Performance Thin Layer Chromatography Approach

Author(s): Ankit Tripathi , Jigni Mishra, Anuja Bhardwaj, Kshipra Misra and Sarada S.K. Sagi*

Volume 7, Issue 2, 2020

Page: [67 - 75] Pages: 9

DOI: 10.2174/2213240606666190725104244

Abstract

Background: Quercetin (3’,3’,4,5,7-pentahydroxyflavonol), a natural flavonoid found in fruits, vegetables, beverages, and other phytoproducts, exerts multiple health benefits including a reduction in hypoxia-induced oxidative stress, inflammation, lipid peroxidation, allergic disorders, neurodegenerative disorders, and cardiovascular diseases.

Objective: Despite knowledge of such therapeutic efficacy of quercetin to human health, there is limited literature available that sheds light on an organ-wise distribution of quercetin. Therefore, the current study was performed to accurately estimate the distribution of quercetin in its supplemented form in different tissues of a mammalian model, i.e., male Sprague Dawley (SD) rats.

Materials and Methods: The rats were exposed to different durations (1 h, 3 h, 6 h, and 12 h) of hypoxia in a simulated hypobaric hypoxia chamber, with parameters maintained at 8 % O2 and 282 mm Hg, following which they were sacrificed. Plasma and different tissue samples were duly collected. A high-performance thin layer chromatography (HPTLC) approach was employed for the first time, using our own reported method, along with an optimized sample preparation procedure for quercetin determination. Briefly, the samples were developed in a mobile phase constituted of ethyl acetate, dichloromethane, methanol, formic acid, and glacial acetic acid.

Results and Dicussion: Distinct bands of quercetin in resultant HPTLC profiles verified that the amount of quercetin varied among different tissues, with varying durations to hypoxia exposure. Quercetin was substantially retained in vital organs namely, lungs, liver, and heart for relatively longer durations.

Conclusion: The present study established HPTLC as an efficient and high throughput tool, leading to a satisfactory evaluation of the amount of quercetin present in various tissue samples under hypoxia.

Keywords: HPTLC, method validation, quercetin, distribution, hypoxia, male SD rats.

[1]
Dajas F. Life or death: neuroprotective and anticancer effects of quercetin. J Ethnopharmacol 2012; 143(2): 383-96.
[http://dx.doi.org/10.1016/j.jep.2012.07.005] [PMID: 22820241]
[2]
Khan F, Niaz K, Maqbool F, et al. Molecular targets underlying the anticancer effects of quercetin: an update. Nutrients 2016; 8(9): 529.
[http://dx.doi.org/10.3390/nu8090529] [PMID: 27589790]
[3]
Du G, Zhao Z, Chen Y, et al. Quercetin protects rat cortical neurons against traumatic brain injury. Mol Med Rep 2018; 17(6): 7859-65.
[http://dx.doi.org/10.3892/mmr.2018.8801] [PMID: 29620218]
[4]
Ahmed OA, Badr-Eldin SM, Tawfik MK, Ahmed TA, El-Say KM, Badr JM. Design and optimization of self-nanoemulsifying delivery system to enhance quercetin hepatoprotective activity in paracetamol-induced hepatotoxicity. J Pharm Sci 2014; 103(2): 602-12.
[http://dx.doi.org/10.1002/jps.23834] [PMID: 24395640]
[5]
Kleemann R, Verschuren L, Morrison M, et al. Anti-inflammatory, anti-proliferative and anti-atherosclerotic effects of quercetin in human in vitro and in vivo models. Atherosclerosis 2011; 218(1): 44-52.
[http://dx.doi.org/10.1016/j.atherosclerosis.2011.04.023] [PMID: 21601209]
[6]
Patir H, Sarada SK, Singh S, Mathew T, Singh B, Bansal A. Quercetin as a prophylactic measure against high altitude cerebral edema. Free Radic Biol Med 2012; 53(4): 659-68.
[http://dx.doi.org/10.1016/j.freeradbiomed.2012.06.010] [PMID: 22743108]
[7]
Hai-li LI. Determination of quercetin and kaempferol in Pteridium aquilinumvar Latiusculum by HPLC. Huaxi Yaoxue Zazhi 2009; 4.
[8]
Wang L, Morris ME. Liquid chromatography-tandem mass spectroscopy assay for quercetin and conjugated quercetin metabolites in human plasma and urine. J Chromatogr B Analyt Technol Biomed Life Sci 2005; 821(2): 194-201.
[http://dx.doi.org/10.1016/j.jchromb.2005.05.009] [PMID: 15925552]
[9]
Cheung HY, Zhang QF. Enhanced analysis of triterpenes, flavonoids and phenolic compounds in Prunella vulgaris L. by capillary zone electrophoresis with the addition of running buffer modifiers. J Chromatogr A 2008; 1213(2): 231-8.
[http://dx.doi.org/10.1016/j.chroma.2008.10.033] [PMID: 18980769]
[10]
Hajmalek M, Goudarzi M, Ghaffari S, Attar H, Mazlaghan MG. Development and validation of a HPTLC method for analysis of Sunitinib malate. Braz J Pharm Sci 2016; 52(4): 595-601.
[http://dx.doi.org/10.1590/s1984-82502016000400003]
[11]
Choma I. Antibiotics: TLC AnalysisEncyclopedia of Chromatography Cazes, J, Ed; CRC Press: Boca Raton. 2010; Vol. 3: pp. 89-95.
[12]
Rosenthal G. Plant nonprotein amino and imino acids: biological, biochemical, and toxicological properties 1st ed Elsevier: New York. 1982.
[13]
Kannamkumarath SS, Wrobel K, Wrobel K. B’Hymer, C.; Caruso, J.A. Capillary electrophoresis-inductively coupled plasma-mass spectrometry: an attractive complementary technique for elemental speciation analysis. J Chromatogr A 2002; 975(2): 245-66.
[http://dx.doi.org/10.1016/S0021-9673(02)01218-9] [PMID: 12456080]
[14]
Sherma J. Review of HPTLC in drug analysis: 1996-2009. J AOAC Int 2010; 93(3): 754-64.
[PMID: 20629372]
[15]
Misra K, Tulsawani R, Shyam R, Meena DK, Morlock G. Hyphenated high-performance thin-layer chromatography for profiling of some Indian natural efficiency enhancers. J Liq Chromatogr Relat Technol 2012; 35(10): 1364-87.
[http://dx.doi.org/10.1080/10826076.2012.675859]
[16]
Mishra J, Hande P, Sharma P, Bhardwaj A, Rajput R, Misra K. Characterization of nucleobases in sea buckthorn leaves: An HPTLC approach. J Liq Chromatogr Relat Technol 2017; 40(1): 50-7.
[http://dx.doi.org/10.1080/10826076.2017.1283517]]
[17]
Rossi Forim M, Perlatti B, Soares Costa E, Facchini Magnani R, Donizetti de Souza G. Concerns and Considerations about the Quality Control of Natural Products Using Chromatographic Methods. Curr Chromatogr 2015; 2(1): 20-31.
[http://dx.doi.org/10.2174/2213240601666141113212732]
[18]
Sangeetha RK, Ravi TK. In vitro Drug-Drug Interaction Studies of Apixaban with Atorvastatin by HPTLC Method. J Drug Metab Toxicol 2018; 9(241): 2.
[19]
Zhang D, Armour E, Sherma J. Development of quantitative HPTLC–densitometry methods following a model approach for transfer of TLC screening methods for pharmaceutical products of cefixime, cefuroxime axetil, cephalexin⋅ H2O, ciprofloxacin HCl, levofloxacin, and metronidazole. Acta Chromatogr 2017; 29(4): 484-6.
[http://dx.doi.org/10.1556/1326.2016.29409]
[20]
Lala LG, D’Mello PM, Naik SR. HPTLC determination of diclofenac sodium from serum. J Pharm Biomed Anal 2002; 29(3): 539-44.
[http://dx.doi.org/10.1016/S0731-7085(02)00131-0] [PMID: 12062654]
[21]
Böhmdorfer S, Oberlerchner JT, Rosenau T, Potthast A. Thin layer chromatography and the analysis of wood derived biomass–a review. Curr Chromatogr 2016; 3(2): 75-85.
[http://dx.doi.org/10.2174/2213240603666160720150830]
[22]
Faiyazuddin M, Rauf A, Ahmad N, et al. A validated HPTLC method for determination of terbutaline sulfate in biological samples: Application to pharmacokinetic study. Saudi Pharm J 2011; 19(3): 185-91.
[http://dx.doi.org/10.1016/j.jsps.2011.03.004] [PMID: 23960758]
[23]
Mishra J, Tripathi A, Misra K, Sarada SKS. HPTLC: A tool for determination of curcumin in mammalian samples. Int J Pharmacogn Phytochem Res 2017; 9(4): 498-501.
[http://dx.doi.org/10.25258/phyto.v9i4.8120]
[24]
Bhardwaj A, Pal M, Srivastava M, Tulsawani R, Sugadev R, Misra K. HPTLC based chemometrics of medicinal mushrooms. J Liq Chromatogr Relat Technol 2015; 38(14): 1392-406.
[http://dx.doi.org/10.1080/10826076.2015.1050501]
[25]
Abourashed EA, Mossa JS. HPTLC determination of caffeine in stimulant herbal products and power drinks. J Pharm Biomed Anal 2004; 36(3): 617-20.
[http://dx.doi.org/10.1016/j.jpba.2004.06.029] [PMID: 15522538]
[26]
Attimarad M, Ahmed KK, Aldhubaib BE, Harsha S. High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery. Pharm Methods 2011; 2(2): 71-5.
[http://dx.doi.org/10.4103/2229-4708.84436] [PMID: 23781433]
[27]
Wang M, Wang C, Han X. Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry-What, how and why? Mass Spectrom Rev 2017; 36(6): 693-714.
[http://dx.doi.org/10.1002/mas.21492] [PMID: 26773411]
[28]
S, Kruger; G, Morlock Applications in Food AnalysisPoole, C Ed; Instrumental Thin Layer Chromatography; Elsevier Science: Ottawa:. 2014; Vol. 18: pp. 407-29.
[29]
Suryakumar G, Gupta A. Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.). J Ethnopharmacol 2011; 138(2): 268-78.
[http://dx.doi.org/10.1016/j.jep.2011.09.024] [PMID: 21963559]
[30]
Nath B, Levin I, Csak T, et al. Hepatocyte-specific hypoxia-inducible factor-1α is a determinant of lipid accumulation and liver injury in alcohol-induced steatosis in mice. Hepatology 2011; 53(5): 1526-37.
[http://dx.doi.org/10.1002/hep.24256] [PMID: 21520168]
[31]
Boesch-Saadatmandi C, Wagner AE, Wolffram S, Rimbach G. Effect of quercetin on inflammatory gene expression in mice liver in vivo - role of redox factor 1, miRNA-122 and miRNA-125b. Pharmacol Res 2012; 65(5): 523-30.
[http://dx.doi.org/10.1016/j.phrs.2012.02.007] [PMID: 22402395]
[32]
Maheshwari DT, Yogendra Kumar MS, Verma SK, Singh VK, Singh SN. Antioxidant and hepatoprotective activities of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves. Food Chem Toxicol 2011; 49(9): 2422-8.
[http://dx.doi.org/10.1016/j.fct.2011.06.061] [PMID: 21723907]
[33]
Molina MF, Sanchez-Reus I, Iglesias I, Benedi J. Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biol Pharm Bull 2003; 26(10): 1398-402.
[http://dx.doi.org/10.1248/bpb.26.1398] [PMID: 14519943]
[34]
Kirar V, Nehra S, Mishra J, Rakhee R, Saraswat D, Misra K. Lingzhi or reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), as a cardioprotectant in an oxygen-deficient environment. Int J Med Mushrooms 2017; 19(11): 1009-21.
[http://dx.doi.org/10.1615/IntJMedMushrooms.2017024584] [PMID: 29345563]
[35]
Chen JY, Hu RY, Chou HC. Quercetin-induced cardioprotection against doxorubicin cytotoxicity. J Biomed Sci 2013; 20(1): 95.
[http://dx.doi.org/10.1186/1423-0127-20-95] [PMID: 24359494]
[36]
Pandey AK, Patnaik R, Muresanu DF, Sharma A, Sharma HS. Quercetin in hypoxia-induced oxidative stress: novel target for neuroprotection. Int Rev Neurobiol 2012; 102: 107-46.
[http://dx.doi.org/10.1016/B978-0-12-386986-9.00005-3] [PMID: 22748828]
[37]
Araneda OF, Tuesta M. Lung oxidative damage by hypoxia. Oxid Med Cell Longev 2012; •••2012856918
[http://dx.doi.org/10.1155/2012/856918] [PMID: 22966417]

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