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Recent Patents on Biotechnology

Editor-in-Chief

ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

Research Article

Nanoliposomes of Supercritical Carbon Dioxide Extract of Small Cardamom Seeds Redresses Type 2 Diabetes and Hypercholesterolemia

Author(s): Kaninika Paul, Paramita Bhattacharjee*, Nilendra Chatterjee and Tapan Kumar Pal

Volume 13, Issue 4, 2019

Page: [284 - 303] Pages: 20

DOI: 10.2174/1872208313666190404101336

Price: $65

Abstract

Background & Objectives: In our previous investigation, oral administration of 1,8- cineole-rich supercritical carbon dioxide extract of small cardamom seeds in Wistar albino rats resulted in achieving normal fasting blood glucose (FBG) and serum cholesterol levels. The objective of this study was to further protect the aforesaid extract and to enhance its in vivo therapeutic efficacies in redressing type 2 diabetes and hypercholesterolemia, by encapsulating it as nanoliposomes. Patents related to nanoliposomes have been revised thoroughly.

Methods: PEGylated nanoliposomes of the aforesaid extract were formulated using soya phosphatidylcholine and Tween 80 by probe-sonication. These nanoliposomes were subjected to in vitro characterizations and were orally administered to Wistar albino rats at three different doses viz. 550, 175 and 55 mg/kg b.w. for detailed investigation of their antidiabetic and hypocholesterolemic efficacies.

Results: FT-IR, DSC and XRD analyses, HLB value (16), entrapment efficiency (84%) and release kinetics (obeying Higuchi model) revealed that the nanoliposomes were o/w type and were hydrophilic. They exhibited appreciable in vitro antioxidant potency (59% DPPH scavenging activity) owing to a synergistic consortium of antioxidants present therein. Oral administration of the liposomes in rats at 550 mg/kg b.w. could restore their normal FBG levels and serum lipid profiles on day 35, with desirable up-down regulations of related key enzymes. The iHOMA2 model could successfully predict the effects of nanoliposomes on insulin sensitivity and glucose uptake in rat liver and brain, respectively.

Conclusion: Nanoliposome of 1,8-cineole rich extract of small cardamom seeds is a new biotherapeutic in redressing type 2 diabetes and hypercholesterolemia.

Keywords: Nanoliposomes, supercritical carbon dioxide, small cardamom seeds, 1, 8-cineole, antioxidant synergy, type 2 diabetes, hypercholesterolemia.

Graphical Abstract

[1]
Farooqi AA, Sreeramu BS, Srinivasappa KN, Eds. In Small cardamom. Hyderabad: Universities press 2005; p. 370.
[2]
Korikontimath VS, Mulge RZ, Achariah JT. Variations in essential oil constituents in high yielding selections of cardamom. J Plant Crops 1999; 27(3): 230-2.
[3]
Messina M, Lampe JW, Birt DF, et al. Reductionism and the narrowing nutrition perspective: Time for re-evaluation and emphasis on food synergy. J Am Diet Assoc 2001; 101(12): 1416-9.
[4]
Bhowal M, Gopal M. Eucalyptol: safety and pharmacological profile. J Pharm Sci 2015; 5(4): 125-31.
[5]
Bisson J, McAlpine JB, Friesen JB, Chen S, Graham J, Pauli GF. Can invalid bioactives undermine natural product-based drug discovery? J Med Chem 2016; 59: 1671-90.
[6]
Paul K, Bhattacharjee P. Mass transfer and hydrodynamic study of supercritical carbon dioxide extraction of 1,8-cineole from small cardamom seeds. Chem Engg Comm 2018; 205: 1023-33.
[7]
Katouzian I, Esfanjani AF, Jafari SM, Akhavan S. Formulation and application of a new generation of lipid nano-carriers for the food bioactive ingredients. Trends Food Sci Technol 2017; 68: 14-25.
[8]
Akbarzadeh A, Rezaei-Sadabady R, Davaran S, et al. Liposome: Classification, preparation, and applications. Nanoscale Res Lett 2013; 8(1): 1-9.
[9]
Rai VK, Mishra N, Agrawal AK, Jain S, Yadav NP. Novel drug delivery system: an immense hope for diabetics. J Drug Delivery 2016; 23(7): 2371-90.
[10]
Oh DK, Lee KY. Preparation of nanoliposomeencapsulating proteins and protein-encapsulated nanoliposome. US7951396B2 2011.
[11]
Venkatraman S, Natarajan JV, Boey YCF, Mehta JS, Howden TTL. NG XW. Nanoliposomes for sustained delivery of tacrolimus for treatment of anterior segment eye diseases. WO2017/184080Al 2017.
[12]
Zhang Y. Method of making liposomes, liposome compositions made by the methods, and methods of using the same. US2012/0171280A1 2012.
[13]
Milla P, Dosio F, Cattel L. PEGylation of proteins and liposomes: A powerful and flexible strategy to improve the drug delivery. Curr Drug Metab 2012; 13(1): 105-19.
[14]
Hill NR, Levy JC, Matthews DR. Expansion of the homeostasis model assessment of b-cell function and insulin resistance to enable clinical trial outcome modeling through the interactive adjustment of physiology and treatment effects: iHOMA2. Diabetes Care 2013; 36(8): 2324-30.
[15]
Ranganna S. Handbook of analysis and quality control for fruits and vegetable products New Delhi. Tata: McGraw Hill 1986; pp. 243-85.
[16]
Ghosh S, Bhattacharjee P, Das S. 1,8-cineol-rich cardamom seed (Elettaria cardamomum) extracts using green technologies and conventional extractions: Process analysis, phytochemical characterization and food application. Sep Sci Technol 2015; 50(13): 1974-85.
[17]
Kuyumcu E, Küçükba FZ. Essential oil composition of Elettaria cardamomum Maton. J Appl Biol Sci 2013; 7(3): 42-5.
[18]
Stein SE. Mass Spectra. Mallard Gaithersburg NIST Chemistry Web Book. In: Linstrom P, Mallard WG, Eds. NIST Standard Reference Database Number 69. Gaithersburg, MD: National Institute of Standards and Technology 2007. [Available at: http://webbbok.nist.gov] [Accessed on: June 2, 2018]
[19]
Adams RP. Identification of essential oil components by gas chromatography/mass spectroscopy. 3rd ed. IL, USA: Allured Publishing Corporation 2007.
[20]
Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium. BMC Complem Altern M 2010; 10(21): 2-8.
[21]
Chakraborty S, Bhattacharjee P. Supercritical carbon dioxide extraction of melatonin from Brassica campestris: In vitro antioxidant, hypocholesterolemic and hypoglycaemic activities of the extracts. Int J Pharm Sci Res 2017; 8(6): 2486-95.
[22]
OECD (Organisation for Economic Co-operation and Development) 423, 2001. Organization of economic co-operation and development guidelines. [Available at:. https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf [Accessed on: May, 2017].
[23]
Lesoin L, Boutin O, Crampon C, Badens E. CO2/water/surfactant ternary systems and liposome formation using supercritical CO2: a review. Colloids Surf A Physicochem Eng Asp 2011; 377(1-3): 1-14.
[24]
Dutta S, Bhattacharjee P. Nanoliposomal encapsulates of piperine-rich black pepper extract obtained by enzyme-assisted supercritical carbon dioxide extraction. J Food Eng 2017; 201: 49-56.
[25]
Patil PY, Jadhav S. In: Joshi MY, Khandekar S, Eds In Preparations of liposomes for drug delivery applications by extrusion of giant unilamellar vesicles. New Delhi: Springer 2015; p. 18.
[26]
Walstar P. In Fennema OR, Ed Dispersed system: basic considerations. New York: Marcel Dekker 1996; p. 103.
[27]
Lampe MA, Burlingame AL, Whitney J, et al. Human stratum corneum lipids: characterization and regional variations. J Lipid Res 1983; 24(2): 120-30.
[28]
Lewis MS, Ullrey DE, Barnard DE, Knapka JJ. In Suckow MA, Weisbroth SH, Franklin CL, Eds Nutrition. Cambridge: Academic Press 2006; pp. 219-301.
[29]
Cheng D, Liang B, Li Y. Antihyperglycemic effect of ginkgo biloba extract in streptozotocin-induced diabetes in rats. BioMed Res Int 2013; 2013: 1-7.
[30]
OECD (Organisation for Economic Co-operation and Development) 425, 2001. Organization of economic co-operation and development guidelines. Available at:. http://www.oecd.org/chemicalsafety/risk-assessment/1948378.pdf [Accessed on: June, 2017].
[31]
Shibib BA, Khan LA, Rahman R. Hypoglycaemic activity of Coccinia indica and Momordica charantia in diabetic rats: Depression of the hepatic gluconeogenic enzymes glucose-6-phosphatase and fructose-1, 6-bisphosphatase and elevation of both liver and red-cell shunt enzyme glucose-6-phosphate dehydrogenase. Biochem J 1993; 292(1): 267-70.
[32]
Ahmad A, Ahmad R. Resveratrol mitigate structural changes and hepatic stellate cell activation in N′-Nitrosodimethylamine-induced liver fibrosis via restraining oxidative damage. Chem Biol Interact 2014; 221: 1-12.
[33]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72(1): 248-54.
[34]
Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972; 47(2): 389-94.
[35]
Roy S, Das K, Mandal S, et al. Asparagus racemosus roots ameliorates acetaminophen induced hepatotoxicity in rats: An experimental, biochemical and histological study. Int J Recent Sci Res 2014; 5: 1192-7.
[36]
Namani S, Gogineni R, Bandi A, Gandham R, Brahmanapally V. Evaluation of Non-HDL cholesterol and total cholesterol/HDL-C ratio, LDL-C/HDL-C ratio as cumulative marker of cardiovascular disease risk in type 2 diabetes mellitus patients. Int J Biomed Res 2016; 7: 680-4.
[37]
Mallick S, Mandal M, Roy S, et al. Effect of phytosterol extract from sesame seed on experimentally Induced hyperlipidemic rats: dose dependent study. Int J Pharma Bio Sci 2016; 7: 370-7.
[38]
Rao AV, Ramakrishnan S. Indirect assessment of hydroxymethylglutaryl-CoA reductase (NADPH) activity in liver tissue. Clin Chem 1975; 21(10): 1523-5.
[39]
Bio-Rad Laboratories. SpectraBase. Available at:. http://spectrabase.com/spectrum/76CgFOBF69G [Accessed on: June 06, 2018].
[40]
Bio-Rad Laboratories. SpectraBase. Available at:. http://spectrabase.com/spectrum/5glko5vslvB (Accessed on: June 06, 2018).
[41]
Boughendjioua H, Djeddi S. Fourier transformed infrared spectroscopy analysis of constituents of lemon essential oils from Algeria. Am J Optics Photon 2017; 5(3): 30-5.
[42]
Bio-Rad Laboratories. SpectraBase. [Available at:. http://spectrabase.com/spectrum/BysYlv5n3w8 [Accessed on: June 06, 2018].
[43]
Dyer JR. Ed In: Infrared spectroscopy. Delhi: Prentice- Hall of India Pvt. Ltd 2012; pp. 143-60.
[44]
Memoli A, Palermiti LG, Travagli V, Alhaique F. Egg and soya phospholipids-sonication and dialysis: a study on liposome characterization. Int J Pharm 1995; 117(2): 159-63.
[45]
Aucoin HR, Wilson AN, Wilson AM, Ishihara K, Guiseppi-Elie A. Release of potassium ion and calcium ion from phosphorylcholine group bearing hydrogels. Polymers 2013; 5(4): 1241-57.
[46]
Haidar ZS, Hamdy RC, Tabrizian M. Protein release kinetics for core-shell hybrid nanoparticles based on the layer-by-layer assembly of alginate and chitosan on liposomes. Biomaterials 2008; 29(9): 1207-15.
[47]
Pasha SR. Evaluation of hypolipidemic activity of methanolic extract of Ficus dalhousiaemiq on triton-x and high fat diet induced hyperlipidemia in albino rats. Eur J Pharm Med Res 2014; 1(1): 183-91.

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