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Current Neurovascular Research

Editor-in-Chief

ISSN (Print): 1567-2026
ISSN (Online): 1875-5739

Research Article

NSE, S100B and MMP9 Expression Following Reperfusion after Carotid Artery Stenting

Author(s): Xiaofan Yuan , Jianhong Wang, Duozi Wang, Shu Yang, Nengwei Yu and Fuqiang Guo*

Volume 16, Issue 2, 2019

Page: [129 - 134] Pages: 6

DOI: 10.2174/1567202616666190321123515

Price: $65

Abstract

Objective: Previous studies have shown that the neuron-specific- enolase (NSE), S100B protein (S100B) and matrix metalloproteinase-9 (MMP9) are specific markers for studying cerebral injury. This study was aimed to demonstrate these biomarkers for their correlation with reperfusion after carotid artery stenting (CAS).

Methods: In this study, a total of 44 patients who were diagnosed unilateral carotid artery stenosis by digital subtraction angiography (DSA) and underwent CAS, were selected as the operation groups. The patients’ blood samples were collected at three different time points: T1, prior to operation; T2, next morning after operation (24 hours); T3, three days after operation (72 hours); All of the patients with the operation received computed tomography perfusion (CTP) at T1 and T3. The second group of 12 patients, who were excluded for carotid artery stenosis by DSA, were assigned to be the control group; Blood samples of these patients were collected at T1. The concentrations of NSE, S100B and MMP9 in serum from patients of both groups were detected by ELISA.

Results: All of the operations were implanted in stents successfully without complications. (1) After CAS, rCBF increased while rMTT and rTTP decreased. (2) The concentrations of NSE, S100B and MMP9 in the serum decreased gradually (T1>T2>T3). There was no significant difference between the control group and the operation group at T1 (P>0.05) on their concentrations of NSE, S100B and MMP9 in the serum. When compared among the operation groups, the concentrations of NSE, S100B and MMP9 in the serum at T1 and T3 showed significant difference (P<0.05). (3) Correlation analysis among the operation groups indicated that NSE, S100B, MMP9 and rCBF were positively correlated before operation (r = 0.69, 0.58 and 0.72, respectively, P < 0.05), as well as after operation (r = 0.75, 0.65 and 0.60, respectively, P < 0.05).

Conclusion: We concluded that the concentrations of NSE, S100B and MMP9 in serum decreased with the improvement of cerebral reperfusion after CAS. NSE, S100B and MMP9 can be used as laboratory biochemical markers to evaluate the improvement of reperfusion after CAS. The results very well complement the imaging methods, such as CTP.

Keywords: NSE, S100B, MMP9, reperfusion, CT perfusion, carotid artery stenting, carotid artery stenosis.

[1]
Powers WJ, Rabinstein AA, Ackerson T, et al. 2018 Guidelines for the early management of patients with acute ischemic stroke. Stroke 2018; 439(3): 509-10.
[2]
Brott TG, Hobson RW, Howard G, et al. Stenting versus endarterectomy for treatment of carotid-artery stenosis. N Engl J Med 2010; 363(1): 11-23.
[3]
Hoeffner EG, Case I, Jain R, et al. Cerebral perfusion CT: Technique and clinical applications. Radiology 2004; 231(3): 632-44.
[4]
Cianfoni A, Colosimo C, Basile M, et al. Brain perfusion CT: Principles, technique and clinical applications. Radiol Med 2007; 112(8): 1225-43.
[5]
Chin SC, Chang CH, Chang TY, et al. Brain computed tomography perfusion may help to detect hemodynamic reconstitution and predict intracerebral hemorrhage after carotid stenting. J Vasc Surg 2012; 56(5): 1281-90.
[6]
Hajduková L, Sobek O, Prchalová D, et al. Biomarkers of brain damage: S100B and NSE concentrations in cerebrospinal fluid-a normative study. BioMed Res Int 2015; 2015(4)379071
[7]
Turner RJ, Sharp FR. Implications of MMP9 for blood brain barrier disruption and hemorrhagic transformation following ischemic stroke. Front Cell Neurosci 2016; 10: 56.
[8]
Sandoval KE, Witt KA. Blood-brain barrier tight junction permeability and ischemic stroke. Neurobiol Dis 2008; 32(2): 200-19.
[9]
Kochanek PM, Berger RP, Bayr H, et al. Biomarkers of primary and evolving damage in traumatic and ischemic brain injury: Diagnosis, prognosis, probing mechanisms, and therapeutic decision making. Curr Opin Crit Care 2008; 14(2): 135-41.
[10]
Tejima E, Zhao BQ, Tsuji K, et al. Astrocytic induction of matrix metalloproteinase-9 and edema in brain hemorrhage. J Cereb Blood Flow Metab 2007; 27(3): 460-8.
[11]
Zetterberg H, Smith DH, Blennow K. Biomarkers of mild traumatic brain injury in cerebrospinal fluid and blood. Nat Rev Neurol 2013; 9(4): 201-10.
[12]
Hayakata T, Shiozaki T, Tasaki O, et al. Changes in CSF S100B and cytokine concentrations in early-phase severe traumatic brain injury. Shock 2004; 22(2): 102-7.
[13]
Waaijer A, Van der Schaaf IC, Velthuis BK, et al. Reproducibility of quantitative CT brain perfusion measurements in patients with symptomatic unilateral carotid artery stenosis. Am J Neuroradiol 2007; 28(5): 927-32.
[14]
Chin SC, Chang CH, Chang TY, et al. Brain computed tomography perfusion may help to detect hemodynamic reconstitution and predict intracerebral hemorrhage after carotid stenting. J Vasc Surg 2012; 56(5): 1281-90.
[15]
Li DD, Song JN, Huang H, et al. The roles of MMP-9/TIMP-1 in cerebral edema following experimental acute cerebral infarction in rat. Neurosci Lett 2013; 550: 168-72.
[16]
Asahi M, Wang X, Mori T, et al. Effects of matrix metalloproteinase-9 gene knock-out on the proteolysis of blood–brain barrier and white matter components after cerebral ischemia. J Neurosci 2001; 21(19): 7724-32.
[17]
Kapural M, Krizanac-Bengez LJ, Barnett G, et al. Serum S-100β as a possible marker of blood–brain barrier disruption. Brain Res 2002; 940(1-2): 102-4.
[18]
Tanaka Y, Marumo T, Omura T, et al. Early increases in serum S100B are associated with cerebral hemorrhage in a rat model of focal cerebral ischemia. Brain Res 2008; 1227: 248-54.
[19]
Sen J, Belli A. S100B in neuropathologic states: The CRP of the brain? J Neurosci Res 2007; 85(7): 1373-80.
[20]
Gautier S, Ouk T, Petrault O, et al. Neutrophils contribute to intracerebral haemorrhages after treatment with recombinant tissue plasminogen activator following cerebral ischaemia. Br J Pharmacol 2009; 156(4): 673-9.
[21]
Zhang JH, Li JK, Ma LL, et al. RNA interference-mediated silencing of S100B improves nerve function recovery and inhibits hippocampal cell apoptosis in rat models of ischemic stroke. J Cell Biochem 2018; 119(10): 8095-111.
[22]
Dragas M, Koncar I, Opacic D, et al. Fluctuations of serum neuron specific enolase and protein S-100B concentrations in relation to the use of shunt during carotid endarterectomy. PLoS One 2015; 10(4)e0124067
[23]
Gonçalves CA, Leite MC, Nardin P. Biological and methodological features of the measurement of S100B, a putative marker of brain injury. Clin Biochem 2008; 41(10-11): 755-63.
[24]
Kimura F, Kadohama T, Kitahara H, et al. Serum neuron-specific enolase level as predictor of neurologic outcome after aortic surgery. Thorac Cardiovasc Surg 2019; 22. [Epub ahead of print].
[25]
Mattusch C, Diederich KW, Schmidt A, et al. Effect of carotid artery stenting on the release of S-100B and neurone-specific enolase. Angiology 2011; 62(5): 376-80.
[26]
Lin L, Wang X, Yu Z. Ischemia-reperfusion injury in the brain: Mechanisms and potential therapeutic strategies. Biochem Pharmacol 2016; 5(4): pii 213.
[27]
Waaijer A, Van der Schaaf IC, Velthuis BK, et al. Reproducibility of quantitative CT brain perfusion measurements in patients with symptomatic unilateral carotid artery stenosis. Am J Neuroradiol 2007; 28(5): 927-32.
[28]
Chin SC, Chang CH, Chang TY, et al. Brain computed tomography perfusion may help to detect hemodynamic reconstitution and predict intracerebral hemorrhage after carotid stenting. J Vasc Surg 2012; 56(5): 1281-90.
[29]
Wintermark M, Flanders AE, Velthuis B, et al. Perfusion-CT assessment of infarct core and penumbra: Receiver operating characteristic curve analysis in 130 patients suspected of acute hemispheric. Stroke 2006; 37: 979-85.
[30]
Yoshie T, Ueda T, Takada T, et al. Prediction of cerebral hyperperfusion syndrome after carotid artery stenting by CT perfusion imaging with acetazolamide challenge. Neuroradiology 2016; 58(3): 253-9.
[31]
Merckel LG, Van der Heijden J, Jongen LM, et al. Effect of stenting on cerebral CT perfusion in symptomatic and asymptomatic patients with carotid artery stenosis. Am J Neuroradiol 2012; 33(2): 280-5.
[32]
Yang B, Chen W, Yang Y, et al. Short-and long-term hemodynamic and clinical effects of carotid artery stenting. Am J Neuroradiol 2012; 33(6): 1170.
[33]
Scarcello E, Morrone F, Piro P, et al. Protein S-100B as biochemical marker of brain ischemic damage after treatment of carotid stenosis. Ann Vasc Surg 2011; 25(7): 975-8.

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