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Current Diabetes Reviews

Editor-in-Chief

ISSN (Print): 1573-3998
ISSN (Online): 1875-6417

Research Article

Intron-specific Single Nucleotide Polymorphisms of Fat Mass and Obesity- Associated Gene in Obese and Overweight Individuals of the Indian Adult Population- A Pilot Study

Author(s): Aakash Reddy, Katari Venkatesh*, Sayani Sahu, Pallavi Sinha Roy, Konkona Datta, Pearlin, L. Vinod Kumar Reddy, Erfath Thanjeem Begum Moghal, Madhubanti Mullick, Gundu H.R. Rao and Dwaipayan Sen*

Volume 16, Issue 1, 2020

Page: [84 - 94] Pages: 11

DOI: 10.2174/2542591901666181126144449

Price: $65

Abstract

Background: The Fat mass and obesity-associated gene (FTO) and its involvement in weight gain and obesity is well-known. However, no reports have been published on the Indian population regarding the relationship between single nucleotide polymorphisms (SNPs) in its intronic region and obesity. The aim of this pilot study was to evaluate the frequency and association of SNPs in intron-1 of the FTO gene in obese and overweight Indian adults.

Methods: This study group consisted of 80 adults, aged 23.5 ± 8.9 yr, with a mean BMI of 28.8 ± 6.2 kg/m2. Genomic DNA was isolated, exons1-3 & intron1 of FTO were amplified using polymerase chain reaction and sequenced by ABI sequencing detection system. The reported SNPs rs1420185, rs8050136, rs1121980 and rs55872725 were checked for their presence or absence in this group of the adult Indian population.

Results: No mutations were found in the exonic sequence of FTO, however, the association of rs1420185, rs8050136, rs1121980 and rs55872725 SNPs was identified in this population. The genotypic frequency at FTO rs8050136 was 32.2% for C>A, at rs55872725 it was 45.7% for C>T, at rs1420185 it was 27.1% for T>C and at rs1121980 it was 30.5% for G>A. All four SNPs in combination were observed in 6 participants (10.2%), all of whom were found to be either obese or overweight.

Conclusion: These findings indicate that Indians with these SNPs are most likely to be at increased risk of obesity.

Keywords: Fat mass and obesity-associated gene, intronic mutations, DNA sequencing, FinchTV, obese, adult.

[1]
Chan RS, Woo J. Prevention of overweight and obesity: how effective is the current public health approach. Int J Environ Res Public Health 2010; 7(3): 765-83.
[2]
Nammi S, Koka S, Chinnala KM, Boini KM. Obesity: An overview on its current perspectives and treatment options. Nutr J 2004; 3: 3.
[3]
Hess ME, Bruning JC. The fat mass and obesity-associated (FTO) gene: Obesity and beyond? Biochim Biophys Acta 2014; 1842(10): 2039-47.
[4]
Lerma-Cabrera JM, Carvajal F, Lopez-Legarrea P. Food addiction as a new piece of the obesity framework. Nutr J 2016; 15: 5.
[5]
Saldaña-Alvarez Y, Salas-Martínez MG, García-Ortiz H, et al. Gender-dependent association of FTO polymorphisms with body mass index in Mexicans. PLoS One 2016; 11(1)e0145984
[6]
Jiao Y, Zhang J, Lu L, Xu J, Qin L. The Fto gene regulates the proliferation and differentiation of pre-adipocytes in vitro. Nutrients 2016; 8(2): 102.
[7]
Ronkainen J, Huusko TJ, Soininen R, et al. Fat mass- and obesity-associated gene Fto affects the dietary response in mouse white adipose tissue. Sci Rep 2015; 5: 9233.
[8]
Harbron J, van der Merwe L, Zaahl MG, Kotze MJ, Senekal M. Fat mass and obesity-associated (FTO) gene polymorphisms are associated with physical activity, food intake, eating behaviors, psychological health, and modeled change in body mass index in overweight/obese Caucasian adults. Nutrients 2014; 6(8): 3130-52.
[9]
Peng S, Zhu Y, Xu F, Ren X, Li X, Lai M. FTO gene polymorphisms and obesity risk: a meta-analysis. BMC Med 2011; 9: 71.
[10]
Hotta K, Nakata Y, Matsuo T, et al. Variations in the FTO gene are associated with severe obesity in the Japanese. J Hum Genet 2008; 53(6): 546-53.
[11]
Albuquerque D, Nóbrega C, Manco L. Association of FTO polymorphisms with obesity and obesity-related outcomes in Portuguese children. PLoS One 2013; 8(1)e54370
[12]
Grant SF, Li M, Bradfield JP, et al. Association analysis of the FTO gene with obesity in children of Caucasian and African ancestry reveals a common tagging SNP. PLoS One 2008; 3(3)e1746
[13]
Peeters A, Beckers S, Verrijken A, et al. Variants in the FTO gene are associated with common obesity in the Belgian population. Mol Genet Metab 2008; 93(4): 481-4.
[14]
Pasupuleti SK, Katari V, Lokanathan S, et al. Novel frame shift mutations (‘A’ deletion) observed in exon 9 of Wilms’ tumor (WT1) gene in a patient reported with glomerulosclerosis. Gene 2014; 546(1): 63-7.
[15]
Bhurosy T, Jeewon R. Overweight and obesity epidemic in developing countries: A problem with diet, physical activity, or socioeconomic status? ScientificWorldJournal 2014; 2014964236
[16]
Sheet WF. Obesity and overweight. World Health Organization WHO 2017 October 2017 [cited on 2017 16-10-2017]; Available from. http://www.who.int/mediacentre/factsheets/fs311/en/
[17]
James WP. What are the health risks? The medical consequences of obesity and its health risks. Exp Clin Endocrinol Diabetes 1998; 106(Suppl. 2): 1-6.
[18]
Pi-Sunyer FX. The medical risks of obesity. Obes Surg 2002; 12(Suppl. 1): 6S-11S.
[19]
Pi-Sunyer X. The medical risks of obesity. Postgrad Med 2009; 121(6): 21-33.
[20]
Van Gaal LF, De Leeuw IH. Human obesity: a medical assessment of health risks. Int J Risk Saf Med 1995; 7(2): 121-34.
[21]
Fawcett KA, Barroso I. The genetics of obesity: FTO leads the way. Trends Genet 2010; 26(6): 266-74.
[22]
Ramos AV, Bastos-Rodrigues L, Resende BA, et al. The contribution of FTO and UCP-1 SNPs to extreme obesity, diabetes and cardiovascular risk in Brazilian individuals. BMC Med Genet 2012; 13: 101.
[23]
Stratigopoulos G, LeDuc CA, Cremona ML, Chung WK, Leibel RL. Cut-like homeobox 1 (CUX1) regulates expression of the fat mass and obesity-associated and retinitis pigmentosa GTPase regulator-interacting protein-1-like (RPGRIP1L) genes and coordinates leptin receptor signaling. J Biol Chem 2011; 286(3): 2155-70.
[24]
Stratigopoulos G, Padilla SL, LeDuc CA, et al. Regulation of Fto/Ftm gene expression in mice and humans. Am J Physiol Regul Integr Comp Physiol 2008; 294(4): R1185-96.
[25]
Fei X, Qin Z, Liang Z. Contribution of CDP/Cux, a transcription factor, to cell cycle progression. Acta Biochim Biophys Sin (Shanghai) 2007; 39(12): 923-30.
[26]
Mailly F, Bérubé G, Harada R, Mao PL, Phillips S, Nepveu A. The human cut homeodomain protein can repress gene expression by two distinct mechanisms: active repression and competition for binding site occupancy. Mol Cell Biol 1996; 16(10): 5346-57.

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