Notoginsenoside R1 Promotes the Growth of Neonatal Rat Cortical Neurons via the Wnt/β-catenin Signaling Pathway

Title:Notoginsenoside R1 Promotes the Growth of Neonatal Rat Cortical Neurons via the Wnt/β-catenin Signaling Pathway

Volume: 17 Issue: 7

Author(s): Xing-Tong Li, Wei Ma, Xian-Bin Wang, Zhang Liang, Jin-Wei Yang, Yuan He, Yun-Fei Dai, Tong Zhang, Jia-Wei Wang, Kuang-Pin Liu, Xiang-Peng Wang, Xing-Kui Zhang, Chun-Yan Li, Jun-Jun Li, Hong-Yan Tang, Ping Su*, Jian-Hui Guo*Li-Yan Li*

Affiliation:

• Department of Neurosurgery, First People`s Hospital of Kunming City, Yunnan Kunming,China

• Second Department of General Surgery, First People`s Hospital of Yunnan Province, Yunnan Kunming,China

• Institute of Neuroscience, Kunming Medical University, Yunnan Kunming,China

Keywords: Wnt/β-catenin signaling pathway, β-catenin, NGR1, growth, cortical neuron, RNA interference.

Abstract: Background & Objective: Notoginsenoside R1 (NGR1) is one of the main effective components of Panax notoginseng.

Method: Primary cortical neurons were harvested from neonatal rats and cultured to analyze the role of NGR1 in neuronal growth and the effects of NGR1 on the Wnt/β-catenin signaling pathway. Following treatment with NGR1, immunocytochemistry was used to detect expression of Tuj1 and MAP2, and RT-qPCR was used to measure mRNA levels of key factors in the Wnt signaling pathway.

Results: Results showed that NGR1 promotes growth of cultured neurons and significantly upregulates mRNA levels of β-catenin, Dishevelled, and Frizzled. To further confirm whether NGR1 promoted cortical neuron growth via the Wnt/β-catenin signaling pathway, we knocked down β- catenin mRNA by siRNA interference; following NGR1 treatment of β-catenin-knockdown neurons, β-catenin mRNA levels increased significantly.

Conclusion: In conclusion, these results demonstrate that NGR1 promotes growth of cultured cortical neurons from the neonatal rat, possibly via the Wnt/β-catenin signaling pathway.

Cite this article as:

Li Xing-Tong, Ma Wei, Wang Xian-Bin, Liang Zhang, Yang Jin-Wei, He Yuan, Dai Yun-Fei, Zhang Tong, Wang Jia-Wei, Liu Kuang-Pin, Wang Xiang-Peng, Zhang Xing-Kui, Li Chun-Yan, Li Jun-Jun, Tang Hong-Yan, Su Ping*, Guo Jian-Hui*, Li Li-Yan*, Notoginsenoside R1 Promotes the Growth of Neonatal Rat Cortical Neurons via the Wnt/β-catenin Signaling Pathway, CNS & Neurological Disorders - Drug Targets 2018; 17 (7) . https://dx.doi.org/10.2174/1871527317666180711093538