Biomedical Potentialities of Taraxacum officinale-based Nanoparticles Biosynthesized Using Methanolic Leaf Extract

Title:Biomedical Potentialities of Taraxacum officinale-based Nanoparticles Biosynthesized Using Methanolic Leaf Extract

Volume: 18 Issue: 14

Author(s): Tahir Rasheed, Muhammad Bilal*, Chuanlong Li and Hafiz M.N. Iqbal*

Affiliation:

• State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240,China

• Tecnologico de Monterrey, School of Engineering and Sciences, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L., CP 64849,Mexico

Keywords: Antibacterial, anti-biofilm, characterization, MCF-7 cell line, nanoparticles, Taraxacum officinale.

Abstract: Background: In the present study, the potential of methanolic leaf extract of Taraxacum officinale plant as a function of bio-inspired green synthesis for the fabrication of silver nanoparticles (AgNPs) has been explored.

Methods: The bio-reduction of aqueous silver nitrate (AgNO3) solution was confirmed by visually detecting the color change from pale yellow to blackish-brown. Maximum absorbance was observed at 420 nm due to the presence of characteristic surface Plasmon resonance of nano silver by UV-visible spectroscopy. The role of various functional groups in the bio-reduction of silver and chemical transformation was verified by Fourier transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) predict the shape (rocky, flack type, ellipsoidal, etc.), size (68 nm) and elemental composition (Ag as a major constituent) of the biosynthesized AgNPs, respectively.

Results: Transmission electron microscopy (TEM) analysis further corroborated the morphology of the AgNPs. Color mapping and atomic force microscopy (AFM) confirmed the nano-sized topography. The dynamic light scattering (DLS) showed the charge, stability, and size of the AgNPs. The generated AgNPs presented potential antibacterial activities against Gram-positive and Gram-negative bacterial strains including Staphylococcus aureus, Escherichia coli, and Haemophilus influenzae. The biosynthesized AgNPs also showed antiproliferative activity against MCF-7 breast cancer cell line in a dosedependent manner.

Conclusion: In conclusion, results clearly indicate that biosynthesized AgNPs could be used as effective nano drug for treating infectious diseases caused by multidrug resistant bacterial strains in the near future.

Cite this article as:

Rasheed Tahir , Bilal Muhammad *, Li Chuanlong and Iqbal M.N. Hafiz *, Biomedical Potentialities of Taraxacum officinale-based Nanoparticles Biosynthesized Using Methanolic Leaf Extract, Current Pharmaceutical Biotechnology 2017; 18 (14) . https://dx.doi.org/10.2174/1389201019666180214145421