Abstract
Objective: Chlorella vulgaris (C. vulgaris), a unicellular green microalga, has been widely used as a food supplement and reported to have antioxidant and anticancer properties. The current study was designed to assess the cytotoxic, apoptotic, and DNA-damaging effects of C. vulgaris growth factor (CGF), hot water C. vulgaris extracts, inlung tumor A549 and NCI-H460 cell lines.
Methods: A549 cells, NCI-H460 cells, and normal human fibroblasts were treated with CGF at various concentrations (0-300 μg/ml) for 24 hr. The comet assay and γH2AX assay showed DNA damage in A549 and NCI-H460 cells upon CGF exposure. Evaluation of apoptosis by the TUNEL assay and DNA fragmentation analysis by agarose gel electrophoresis showed that CGF induced apoptosis in A549 and NCI-H460 cells. Results: Chlorella vulgaris hot water extract induced apoptosis and DNA damage in human lung carcinoma cells. Conclusion: CGF can thus be considered a potential cytotoxic or genotoxic drug for treatment of lung carcinoma.Keywords: Apoptosis, C. vulgarisgro with factor (CGF), cytotoxicity, DNA damage, lung carcinoma, medicinal plants, nonsmall cell lung carcinoma.
Graphical Abstract
Medicinal Chemistry
Title:Chlorella vulgaris Induces Apoptosis of Human Non-Small Cell Lung Carcinoma (NSCLC) Cells
Volume: 13 Issue: 6
Author(s): Zhi-Dong Zhang*, Kai Liang, Kun Li, Guo-Quan Wang, Ke-Wei Zhang, Lei Cai*, Shui-Ting Zhai and Kuo-Chen Chou
Affiliation:
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center of Genetics and Development, Shanghai Jiaotong University, Shanghai 200030,China
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center of Genetics and Development, Shanghai Jiaotong University, Shanghai 200030,China
Keywords: Apoptosis, C. vulgarisgro with factor (CGF), cytotoxicity, DNA damage, lung carcinoma, medicinal plants, nonsmall cell lung carcinoma.
Abstract: Objective: Chlorella vulgaris (C. vulgaris), a unicellular green microalga, has been widely used as a food supplement and reported to have antioxidant and anticancer properties. The current study was designed to assess the cytotoxic, apoptotic, and DNA-damaging effects of C. vulgaris growth factor (CGF), hot water C. vulgaris extracts, inlung tumor A549 and NCI-H460 cell lines.
Methods: A549 cells, NCI-H460 cells, and normal human fibroblasts were treated with CGF at various concentrations (0-300 μg/ml) for 24 hr. The comet assay and γH2AX assay showed DNA damage in A549 and NCI-H460 cells upon CGF exposure. Evaluation of apoptosis by the TUNEL assay and DNA fragmentation analysis by agarose gel electrophoresis showed that CGF induced apoptosis in A549 and NCI-H460 cells. Results: Chlorella vulgaris hot water extract induced apoptosis and DNA damage in human lung carcinoma cells. Conclusion: CGF can thus be considered a potential cytotoxic or genotoxic drug for treatment of lung carcinoma.Export Options
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Cite this article as:
Zhang Zhi-Dong*, Liang Kai , Li Kun, Wang Guo-Quan , Zhang Ke-Wei , Cai Lei *, Zhai Shui-Ting and Chou Kuo-Chen , Chlorella vulgaris Induces Apoptosis of Human Non-Small Cell Lung Carcinoma (NSCLC) Cells, Medicinal Chemistry 2017; 13 (6) . https://dx.doi.org/10.2174/1573406413666170510102024
DOI https://dx.doi.org/10.2174/1573406413666170510102024 |
Print ISSN 1573-4064 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6638 |
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