MiR-106a Associated with Diabetic Peripheral Neuropathy Through the Regulation of 12/15-LOX-meidiated Oxidative/Nitrative Stress

Title:MiR-106a Associated with Diabetic Peripheral Neuropathy Through the Regulation of 12/15-LOX-meidiated Oxidative/Nitrative Stress

Volume: 14 Issue: 2

Author(s): Yuanbo Wu, Dandan Xu, Xiang Zhu, Guangwei Yang and Mingshan Ren*

Affiliation:

• Department of Neurology, Provincial Hospital Affiliated to Anhui Medical University, HeFei 230001, Anhui Province,China

Keywords: Diabetic peripheral neuropathy, oxidative stress, nitrative stress, 12/15-LOX, micro-106a, diabetic mellitus.

Abstract: Background: Diabetic peripheral neuropathy (DPN) is a common complication of diabetes with a complex pathogenesis. Study has reported that oxidative stress and nitrative stress are all involved in the DPN. However, the mechanism between them is still unclear. In the present study, we investigated whether miR-106a regulated 12/15-Lipoxygenase (12/15-LOX) of oxidative/nitrative stress (OS/NS) in DPN.

Methods: Dorsal root ganglion (DRG) was isolated from 10 healthy mice and 10 DPN mice. DPN mouse model was established by the injection of streptozotocin (STZ), and high glucose was used for inducing the in vitro model of DPN.

Results: In DPN mice, the levels of fasting blood-glucose (FBG) level, Methane Dicarboxylic Aldehyde (MDA) and the Inducible Nitric Oxide Synthase (iNOS) were increased, while the levels of motor nerve conduction velocity (MNCV), sensory nerve conduction velocity (SNCV) and superoxide dismutase (SOD) were decreased. MiR-106a level in DRG cells of DPN was decreased. The 12/15-LOX expression and cell apoptosis were increased. DRG cells subjected to high glucose resulted in the same effects as above. MiR-106a was observed to target 12/15-LOX and regulated its expression. MiR-106a overexpression reversed the effect that was induced by high glucose, however, overexpression of 12/15-LOX reversed the effect that was induced by miR-106a overexpression.

Conclusion: MiR-106 may be associated with 12/15-LOX mediated OS/NS in DPN and may be considered as a potential therapeutic target.

Cite this article as:

Wu Yuanbo , Xu Dandan , Zhu Xiang, Yang Guangwei and Ren Mingshan*, MiR-106a Associated with Diabetic Peripheral Neuropathy Through the Regulation of 12/15-LOX-meidiated Oxidative/Nitrative Stress, Current Neurovascular Research 2017; 14 (2) . https://dx.doi.org/10.2174/1567202614666170404115912

DOI https://dx.doi.org/10.2174/1567202614666170404115912	Print ISSN 1567-2026
Publisher Name Bentham Science Publisher	Online ISSN 1875-5739