Anticancer and Cytotoxic Activities of [Cu(C6H16N2O2)2][Ni(CN)4] and [Cu(C6H16N2O2)Pd(CN)4] Cyanidometallate Compounds on HT29, HeLa, C6 and Vero Cell Lines

Title:Anticancer and Cytotoxic Activities of [Cu(C6H16N2O2)2][Ni(CN)4] and [Cu(C6H16N2O2)Pd(CN)4] Cyanidometallate Compounds on HT29, HeLa, C6 and Vero Cell Lines

Volume: 17 Issue: 6

Author(s): Ali Aydın*, Sengul Aslan Korkmaz, Veysel Demir and Saban Tekin

Affiliation:

• Department of Molecular Biology and Genetics, Faculty of Science, Gaziosmanpaşa University, P.O. Box: 60240, Tokat,Turkey

Keywords: Cyanidometallate compound, palladium, copper, nickel, antiproliferative effect, apoptosis.

Abstract: Background: In cancer, apoptosis relevant proteins—such as CaM kinase, Bcl-2 or P53, topoisomerase I, cell migration feature and DNA/BSA—macromolecules represent significant targets for current chemotherapeutics.

Objective: We recently reported two coordination compounds—[Cu(C6H16N2O2)2][Ni(CN)4] (1) and [Cu(C6H16 N2O2)Pd(CN)4] (2)—together with their IR spectra, magnetic properties, thermal analyses and crystal structures. Herein, we describe the ability of these complexes to induce apoptosis in relevant proteins and stimulate topoisomerase I activity, cell migration velocity and DNA/BSA binding properties.

Method: The in vitro antiproliferative effects and cell toxicity of both compounds were investigated through pharmacological measurement techniques, and interactions between both compounds and CT-DNA/BSA were studied with UV-Vis spectroscopy and fluorescence spectroscopy.

Results & Conclusion: Studies on cells revealed that 2 (i) demonstrated a high antiproliferative effect, which was higher toward HeLa and C6 cancer cells than toward healthy Vero cells; (ii) impaired the migration of HeLa cells; (iii) altered the P53-Bcl-2 ratio in favor of apoptosis; (iv) strongly bound to DNA/BSA macromolecules; and (v) inhibited human topoisomerase I and KpnI or BamHI restriction endonucleases. In conclusion, this preliminary information demonstrates that 2 may represent a promising antiproliferative agent and a potential candidate for a therapeutic approach against HeLa.

Cite this article as:

Aydın Ali*, Korkmaz Aslan Sengul, Demir Veysel and Tekin Saban, Anticancer and Cytotoxic Activities of [Cu(C6H16N2O2)2][Ni(CN)4] and [Cu(C6H16N2O2)Pd(CN)4] Cyanidometallate Compounds on HT29, HeLa, C6 and Vero Cell Lines, Anti-Cancer Agents in Medicinal Chemistry 2017; 17 (6) . https://dx.doi.org/10.2174/1871520617666170103102417

DOI https://dx.doi.org/10.2174/1871520617666170103102417	Print ISSN 1871-5206
Publisher Name Bentham Science Publisher	Online ISSN 1875-5992