Abstract
Peptide drugs have received more attention as potential next-generation drugs or drug leads. Such attention is primarily due to the higher target selectivities and efficiencies of peptide drugs. Moreover, peptide drugs typically have lower accumulation rates and toxicities. Although peptide drugs have advanced significantly in the 21st century, two bottlenecks (disadvantages) primarily hinder peptide drug research and development compared to small molecule drugs. One bottleneck is the identification of a valuable peptide in a complicated pool of crude products; the other bottleneck is the ability to prepare sufficient amounts of peptides for preclinical or clinical trials. This review discusses important methodologies for addressing these two bottlenecks.
Keywords: Extracellular expression, intracellular expression, in vitro screening, in vivo screening, methodology, peptide.
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Current Proteomics
Title:The Development of High-throughput Identification and Heterologous Expression of Valuable Peptides/Proteins
Volume: 14 Issue: 1
Author(s): Chang-Jun Liu, Lei Wu, Er Meng and Dong-Yi Zhang
Affiliation:
Keywords: Extracellular expression, intracellular expression, in vitro screening, in vivo screening, methodology, peptide.
Abstract: Peptide drugs have received more attention as potential next-generation drugs or drug leads. Such attention is primarily due to the higher target selectivities and efficiencies of peptide drugs. Moreover, peptide drugs typically have lower accumulation rates and toxicities. Although peptide drugs have advanced significantly in the 21st century, two bottlenecks (disadvantages) primarily hinder peptide drug research and development compared to small molecule drugs. One bottleneck is the identification of a valuable peptide in a complicated pool of crude products; the other bottleneck is the ability to prepare sufficient amounts of peptides for preclinical or clinical trials. This review discusses important methodologies for addressing these two bottlenecks.
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Cite this article as:
Liu Chang-Jun, Wu Lei, Meng Er and Zhang Dong-Yi, The Development of High-throughput Identification and Heterologous Expression of Valuable Peptides/Proteins, Current Proteomics 2017; 14 (1) . https://dx.doi.org/10.2174/1570164614666161207162711
DOI https://dx.doi.org/10.2174/1570164614666161207162711 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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