Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/ Angiopoietin-2/Thrombospondin-1 Signaling

Title:Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/ Angiopoietin-2/Thrombospondin-1 Signaling

Volume: 16 Issue: 3

Author(s): Zhiqiang Zhang*, Cong Li, Qijia Tan, Caijun Xie, Yanying Yang, Wengang Zhan, Fu Han, Hari Shanker Sharma*Aruna Sharma

Affiliation:

• Director of Research, CNS Injury & Repair (IECNSIR), Uppsala University Hospital, Uppsala University, Frödingsgatan 12, Bldg. 1228, SE-7541 Uppsala,Sweden

• Director of Research, CNS Injury & Repair (IECNSIR), Uppsala University Hospital, Uppsala University, Frödingsgatan 12, Bldg. 1228, SE-7541 Uppsala,Sweden

Keywords: Curcumin, glioma, angiogenesis, vascular endothelial growth factor.Curcumin, glioma, angiogenesis, vascular endothelial growth factor.

Abstract: Purpose: To investigate the effect of curcumin on tumor growth and angiogenesis of human gliomas and identify the underlying molecular mechanisms.

Methods: A mouse xenograft glioma model was established by subcutaneously inoculating tumor cell aggregates derived from the U87 cell line. Mice were treated with 0.01ml/g body weight of curcumin or saline. Tumor volume was measured. Microvessel density was assessed by CD34 immunostaining, and angiogenesis by immunohistochemical staining of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2) and thrombospondin 1 (TSP-1).

Results: At 28 days after treatment, tumor weights in the curcumin-treated group were much smaller than in the control group (0.23±0.11g vs 0.44±0.15g，p＜0.05), resulting in a 45.8% inhibition of tumor growth. Curcumin also markedly inhibited microvessel density. Expression of VEGF and Ang-2 was inhibited by curcumin, whereas TSP-1 expression was up-regulated.

Conclusion: This study shows that curcumin inhibits tumor growth by inhibiting VEGF/Ang-2/TSP-1- mediated angiogenesis in a xenograft glioma mouse model.

Cite this article as:

Zhang Zhiqiang*, Li Cong, Tan Qijia, Xie Caijun, Yang Yanying, Zhan Wengang, Han Fu, Sharma Shanker Hari*, Sharma Aruna, Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/ Angiopoietin-2/Thrombospondin-1 Signaling, CNS & Neurological Disorders - Drug Targets 2017; 16 (3) . https://dx.doi.org/10.2174/1871527315666160902144513