Antiproliferative and Apoptotic Effect of Dendrosomal Curcumin Nanoformulation in P53 Mutant and Wide-Type Cancer Cell Lines

Title:Antiproliferative and Apoptotic Effect of Dendrosomal Curcumin Nanoformulation in P53 Mutant and Wide-Type Cancer Cell Lines

Volume: 17 Issue: 5

Author(s): Maryam Montazeri, Younes Pilehvar-Soltanahmadi, Mina Mohaghegh, Alireza Panahi, Samaneh Khodi, Nosratollah Zarghami*Majid Sadeghizadeh*

Affiliation:

• Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz,Iran

• Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran,Iran

Keywords: Dendrosomal curcumin (DNC), p53 mutant, apoptosis, breast cancer, colon cancer.

Abstract: Objective: The aim of this paper is to investigate the effect of dendrosomal curcumin (DNC) on the expression of p53 in both p53 mutant cell lines SKBR3/SW480 and p53 wild-type MCF7/HCT116 in both RNA and protein levels.

Background: Curcumin, derived from Curcumin longa, is recently considered in cancer related researches for its cell growth inhibition properties. p53 is a common tumor-suppressor gene involved in cancers and its mutation not only inhibits tumor suppressor activity but also promotes oncogenic activity.

Method: Here, p53 mutant/Wild-type cells were employed to study the toxicity of DNC using MTT assay, Flow cytometry and Annexin-V, Real-time PCR and Western blot were used to analyze p53, BAX, Bcl-2, p21 and Noxa changes after treatment.

Results: During the time, DNC increased the SubG1 cells and decreased G1, S and G2/M cells, early apoptosis also indicated the inhibition of cell growth in early phase. Real-Time PCR assay showed an increased mRNA of BAX, Noxa and p21 during the time with decreased Bcl-2. The expression of p53 mutant decreased in SKBR3/SW480, and the expression of p53 wild-type increased in MCF7/HCT116.

Conclusion: Consequently, p53 plays an important role in mediating the survival by DNC, which can prevent tumor cell growth by modulating the expression of genes involved in apoptosis and proliferation.

Cite this article as:

Montazeri Maryam, Pilehvar-Soltanahmadi Younes, Mohaghegh Mina, Panahi Alireza, Khodi Samaneh, Zarghami Nosratollah*, Sadeghizadeh Majid*, Antiproliferative and Apoptotic Effect of Dendrosomal Curcumin Nanoformulation in P53 Mutant and Wide-Type Cancer Cell Lines, Anti-Cancer Agents in Medicinal Chemistry 2017; 17 (5) . https://dx.doi.org/10.2174/1871520616666160815124537

DOI https://dx.doi.org/10.2174/1871520616666160815124537	Print ISSN 1871-5206
Publisher Name Bentham Science Publisher	Online ISSN 1875-5992