Determining Concentrations of Icotinib in Plasma of Rat by UPLC Method with Ultraviolet Detection: Applications for Pharmacokinetic Studies

Title:Determining Concentrations of Icotinib in Plasma of Rat by UPLC Method with Ultraviolet Detection: Applications for Pharmacokinetic Studies

Volume: 13 Issue: 4

Author(s): Jianhua Xiong*, Hua Ye, Yuxian Lin, Jinzhao Yang, Dingwen Chen, Deguan Yu and Lianguo Chen*

Affiliation:

• Department of Pharmacy, Wenzhou people’s hospital, Wenzhou, 325000, Zhejiang, P.R.,China

• Department of Pharmacy, Wenzhou people’s hospital, Wenzhou, 325000, Zhejiang, P.R,China

Keywords: UPLC, icotinib, pharmacokinetic, rat, plasma, EGFR-TKI.

Abstract: Introduction: In our research, a rapid, selective and sensitive analytical method based on ultra-performance liquid chromatography (UPLC) was developed and validated for the quantitative analysis of icotinib (an oral and novel EGFR-TKI) in rat plasma. This method was applied for pharmacokinetics. Sample preparation was completed through protein precipitation with acetonitrile, and chromatographic separation was performed on an Acquity UPLC BEH C18 column (2.1 mm x 50 mm, 1.7 µm) with a water-acetonitrile-0.1% trifluoroacetic acid mobile phase (0.3 mL/min), utilizing ultraviolet detection at 248 nm. Just 100 µL plasma sample and 3.5 minutes’ running time were required, representing an obvious improvement compared with previously described methods. The assay was linear over the range of 0.05-10 µg/mL for icotinib, with a lower limit of quantitation (LLOQ) of 0.05 μ g/mL for icotinib and an excellent linearity (r²=0.9992) was achieved.

Conclusion: Intra and inter-day precisions of icotinib were no more than 10% and its accuracies were in the range of 98.3-104.0%. Our method was successfully applied for the determination of icotinib in rat plasma for pharmacokinetic study.

Cite this article as:

Xiong Jianhua *, Ye Hua , Lin Yuxian , Yang Jinzhao , Chen Dingwen , Yu Deguan and Chen Lianguo *, Determining Concentrations of Icotinib in Plasma of Rat by UPLC Method with Ultraviolet Detection: Applications for Pharmacokinetic Studies, Current Pharmaceutical Analysis 2017; 13 (4) . https://dx.doi.org/10.2174/1573412912666160815115244