Stimulation of Tumor-Specific Immunity by p5 HER-2/neu Generated Peptide Encapsulated in Nano-liposomes with High Phase Transition Temperature Phospholipids

Title:Stimulation of Tumor-Specific Immunity by p5 HER-2/neu Generated Peptide Encapsulated in Nano-liposomes with High Phase Transition Temperature Phospholipids

Volume: 14 Issue: 4

Author(s): Mona Yazdani, Seyed Amir Jalali, Ali Badiee, Sheida Shariat, Mercedeh Mansourian, Leila Arabi, Azam Abbasi, Zahra Saberi and Mahmoud Reza Jaafari*

Affiliation:

• Biotechnology Research Center, Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad,Iran

Keywords: Cancer vaccine, cell mediated immunity, liposomes, nanoliposomes, P5 HER2/neu peptide, phase transition temperature.

Abstract: Background: The aim of this study is to evaluate the possible advantages of liposomes with high transition temperature (Tm) in the function of a vaccine for P5 HER2/neu-generated peptide and its adjuvant action to elicit CD8+ T cell response and its efficacy in TUBO in vivo tumor mice model, which over expresses the HER2/neu oncogene. P5, a hydrophobic peptide, was encapsulated in the nanoliposomes consisting of DSPC/DSPG/Chol(Tm 54°C) with a chaotropic loading system via 7M urea and described by size, zeta potential, encapsulation efficiency, and the structural stability of SDS-PAGE.

Methods: We immunized the mice for three times subcutaneously based on a two-week intervals using encapsulated peptide in the nanoliposomes, empty liposome, P5 in PBS, and PBS. Enzyme-linked immunospot assay, cytotoxicity test, and flow cytometric studies followed by the size of tumor and survival time measurements, which were done in TUBO tumor mice version.

Results: Findings of ELISpot and flow cytometric analysis showed that immunization with Lip-p5 nanoliposomes has enhanced the antigen-specific IFN-γ response of CD8+ T cells and induced CTL response, which resulted in a smaller tumor and longer survival time. In addition to increase in amounts of IFN-γ-CD8+ T cells in a group, which was immunized with Lip-P5, our findings also revealed a Th1 shift in the group immunized with an empty liposome with reduced frequencies of IL-4-producing cells and increase of IFN-γ-producing cells.

Conclusion: The results indicated that simple liposomes consisting of phospholipids with high transition temperature could be an effective vaccine vehicle for tumor-associated antigens for inducing cell mediate immunity.

Cite this article as:

Yazdani Mona, Jalali Amir Seyed, Badiee Ali, Shariat Sheida, Mansourian Mercedeh, Arabi Leila, Abbasi Azam, Saberi Zahra and Jaafari Reza Mahmoud*, Stimulation of Tumor-Specific Immunity by p5 HER-2/neu Generated Peptide Encapsulated in Nano-liposomes with High Phase Transition Temperature Phospholipids, Current Drug Delivery 2017; 14 (4) . https://dx.doi.org/10.2174/1567201813666160712164149

DOI https://dx.doi.org/10.2174/1567201813666160712164149	Print ISSN 1567-2018
Publisher Name Bentham Science Publisher	Online ISSN 1875-5704