Physico-chemical and Biological Evaluation of Flavonols: Fisetin, Quercetin and Kaempferol Alone and Incorporated in beta Cyclodextrins

Title:Physico-chemical and Biological Evaluation of Flavonols: Fisetin, Quercetin and Kaempferol Alone and Incorporated in beta Cyclodextrins

Volume: 17 Issue: 4

Author(s): Danciu Corina*, Florina Bojin, Rita Ambrus, Delia Muntean, Codruta Soica, Virgil Paunescu, Mirabela Cristea, Iulia Pinzaru and Cristina Dehelean

Affiliation:

• Department of Pharmacognosy, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Square, No. 2, 300041 Timisoara,Romania

Keywords: Fisetin, quercetin, kaempferol, beta- cyclodextrins, chemopreventive, antimicrobial.

Abstract: Background: Fisetin,quercetin and kaempferol are among the important representatives of flavonols, biological active phytocomounds, with low water solubility.

Objective: To evaluate the antimicrobial effect, respectively the antiproliferative and pro apoptotic activity on the B164A5 murine melanoma cell line of pure flavonols and their beta cyclodextrins complexes.

Method: Incorporation of fisetin, quercetin and kaempferol in beta cyclodextrins was proved by scanning electron microscopy (SEM), differencial scanning calorimetry (DSC) and X-ray powder diffraction (XRPD). Pure compounds and their complexes were tested for antiproliferative (MTT) and pro-apoptotic activity (Annexin V-PI) on the B164A5 murine melanoma cell line and for the antimicrobial properties (Disk Diffusion Method) on the selected strains.

Results: The phytocompounds presented in a different manner in vitro chemopreventive activity against B164A5 murine melanoma cell line and weak antimicrobial effect.

Conclusion: The three flavonols: fisetin, quercetin and kaempferol were successfully incorporated in beta-cyclodextrin (BCD) and hydroxylpropyl-beta-cyclodextrin (HPBCD). Incorporation in beta cyclodextrins had a mix effect on the biological activity conducing to decrease, increase or consistent effect compared to pure phytocompound, depending on the screened process and on the chosen combination.

Cite this article as:

Corina Danciu*, Bojin Florina, Ambrus Rita, Muntean Delia, Soica Codruta, Paunescu Virgil, Cristea Mirabela, Pinzaru Iulia and Dehelean Cristina, Physico-chemical and Biological Evaluation of Flavonols: Fisetin, Quercetin and Kaempferol Alone and Incorporated in beta Cyclodextrins, Anti-Cancer Agents in Medicinal Chemistry 2017; 17 (4) . https://dx.doi.org/10.2174/1871520616666160621105306

DOI https://dx.doi.org/10.2174/1871520616666160621105306	Print ISSN 1871-5206
Publisher Name Bentham Science Publisher	Online ISSN 1875-5992