In Vitro Anticancer Effects of Two New Potent Hydrazide Compounds on Leukemic Cells

Title:In Vitro Anticancer Effects of Two New Potent Hydrazide Compounds on Leukemic Cells

Volume: 16 Issue: 12

Author(s): Shahrzad Tavakolfar, Elham Mousavi, Ali Almasirad, Amir Amanzadeh, Seyyed Mohamamd Atyabi, Parichehr Yaghamii, Somayeh Samiee-Sadr and Mona Salimi

Affiliation:

Keywords: Leukemia, apoptosis, cancer, hydrazide.

Abstract: Background: Chronic myeloid leukemia (CML), also recognized as chronic myelogenous leukemia, is initiated in some types of blood-forming cells of the bone marrow. The therapeutic approach to CML is usually chemotherapy; however, severe side effects and complications are major problems in the clinical research. Thus, recent efforts have focused on the search for compounds affecting apoptosis in this type of cancer.

Objective: In this study, in vitro anticancer activity of two compounds (A and B) consisting of a hydrazide backbone with nitro-thiophen and furan substituents was assessed against K562 cell line displaying certain levels of sensitivity to pro-apoptotic compounds.

Methods: The anticancer activity was assessed using MTT assay, flowcytometry, annexin-V and Western blot analysis.

Results: Compounds A and B were both active and revealed a remarkable in vitro cytotoxic effect showing IC50 values of 0.09 and 0.07 μM, respectively, after 72 h of treatment. A significant increase in annexin-V/PI staining, sub-G1 population and Bax/Bcl-2 ratio revealed the apoptotic cell death of compounds A- and B-treated K562 cells.

Conclusion: The results presented here could be used as a first step for the development of powerful chemotherapeutic agents to treat leukemia.

Cite this article as:

Tavakolfar Shahrzad, Mousavi Elham, Almasirad Ali, Amanzadeh Amir, Atyabi Mohamamd Seyyed, Yaghamii Parichehr, Samiee-Sadr Somayeh and Salimi Mona, In Vitro Anticancer Effects of Two New Potent Hydrazide Compounds on Leukemic Cells, Anti-Cancer Agents in Medicinal Chemistry 2016; 16 (12) . https://dx.doi.org/10.2174/1871520616666160404112945