Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry: An Attractive and Prospective Method for Quantitative Bioanalysis in Drug Metabolism

Title:Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry: An Attractive and Prospective Method for Quantitative Bioanalysis in Drug Metabolism

Volume: 17 Issue: 4

Author(s): Zheng Li, Jie Han, Shi-an Sun, Kai Chen and Dao-quan Tang

Affiliation:

Keywords: drug metabolism, HILIC-MS/MS, metabolites, pharmacokinetic study, quantitative bioanalysis, sample preparation.

Abstract: Background: During the development, dosage optimization and safety evaluation of a drug, rapid and precise monitoring of an administered drug and/or its metabolites in biological samples. As drug biotransformation produces more hydrophilic metabolites for the enhancement of drug elimination, it often presents a challenge for traditional reversed-phase liquid chromatography (RPLC) separation. Because hydrophilic interaction liquid chromatography (HILIC) is capable of retaining polar compounds and is readily compatible with mass spectrometry (MS), HILIC has been used as a complementary separation technique to RPLC for the analysis of polar metabolites, especially polar drugs and their metabolites.

Methods: We undertook a comprehensive search of databases for HILIC-related research literature. The characteristics including method, result and innovation of screened papers were described, and an inductive and analytical method was applied to analyze those studies using a reasonable framework.

Results: This paper covers core aspects of the HILIC-MS/MS method and the overall profile of its application in drug and/or its metabolites analysis by reviewing 102 papers. The emphasis of this paper has been placed on the applications of the HILIC-MS/MS method in quantitative bioanalysis of drugs alone or along with their metabolites in drug metabolism studies in recent years. As a fundamental and critical step of the bioanalytical method, conventional sample preparation techniques of biological matrices for the HILIC-MS/MS analysis of drugs and/or their metabolites are also briefly featured.

Conclusion: The findings of this review confirm the powder of HILIC-MS/MS methods in the separation and quantification of drugs and/or their metabolites.

Cite this article as:

Li Zheng, Han Jie, Sun Shi-an, Chen Kai and Tang Dao-quan, Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry: An Attractive and Prospective Method for Quantitative Bioanalysis in Drug Metabolism, Current Drug Metabolism 2016; 17 (4) . https://dx.doi.org/10.2174/1389200217666151210141757