Comparative Protein Profiling of Intraphagosomal Expressed Proteins of Mycobacterium bovis BCG

Title:Comparative Protein Profiling of Intraphagosomal Expressed Proteins of Mycobacterium bovis BCG

Volume: 23 Issue: 1

Author(s): Neelja Singhal, Manish Kumar, Divakar Sharma and Deepa Bisht

Affiliation:

Keywords: Intraphagosomal, MALDI-TOF, M. bovis BCG, proteomics, two-dimensional gel electrophoresis, vaccine.

Abstract: BCG, the only available vaccine against tuberculosis affords a variable protection which wanes with time. In this study we have analyzed and compared the proteins which are expressed differentially during broth-culture and intraphagosomal growth of M.bovis BCG. Eight proteins which showed increased expression during the intraphagosomal growth were identified by MALDI-TOF/MS. These were - a precursor of alanine and proline-rich secreted protein apa, isoforms of malate dehydrogenase, large subunit alpha (Alpha-ETF) of electron transfer flavoprotein, immunogenic protein MPB64 precursor, UPF0036 protein, and two proteins with unknown function. Based on these findings we speculate that higher expression of these proteins has a probable role in intracellular survival, adaptation and/or immunoprotective effect of BCG. Further, these proteins might also be used as gene expression markers for endosome trafficking events of BCG.

Cite this article as:

Singhal Neelja, Kumar Manish, Sharma Divakar and Bisht Deepa, Comparative Protein Profiling of Intraphagosomal Expressed Proteins of Mycobacterium bovis BCG, Protein & Peptide Letters 2016; 23 (1) . https://dx.doi.org/10.2174/0929866523666151106123121