Abstract
Annulated sea snake, Hydrophis cyanocinctus, venom was investigated using reversed-phase high-performance liquid chromatography coupled to electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). MALDI-TOF/TOF identified three compounds (a short-chain neurotoxin, a long-chain neurotoxin and a basic phospholipase A2); whose molecular weights were estimated to be 7285, 10838, and 14235 Da, respectively. Moreover, evolutionary relationship of each protein (identity more than 70%) was separately investigated via phylogenetic trees. This study was done as complementary investigation on the composition of the venom of H. cyanocinctus, the annulated sea snake from the Persian Gulf.
Keywords: Persian Gulf, phospholipase A2, sea snake, three-finger toxin, venom.
Graphical Abstract
Current Proteomics
Title:Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF
Volume: 12 Issue: 1
Author(s): Saeedeh Mosafer Khorjestan, Behrooz Abtahi, Seyed Omid Ranei Siadat, Mohammad Motevalli, Hassan Rezadoost, Parviz Ghezellou and Alireza Ghassempour
Affiliation:
Keywords: Persian Gulf, phospholipase A2, sea snake, three-finger toxin, venom.
Abstract: Annulated sea snake, Hydrophis cyanocinctus, venom was investigated using reversed-phase high-performance liquid chromatography coupled to electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). MALDI-TOF/TOF identified three compounds (a short-chain neurotoxin, a long-chain neurotoxin and a basic phospholipase A2); whose molecular weights were estimated to be 7285, 10838, and 14235 Da, respectively. Moreover, evolutionary relationship of each protein (identity more than 70%) was separately investigated via phylogenetic trees. This study was done as complementary investigation on the composition of the venom of H. cyanocinctus, the annulated sea snake from the Persian Gulf.
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Mosafer Khorjestan Saeedeh, Abtahi Behrooz, Ranei Siadat Seyed Omid, Motevalli Mohammad, Rezadoost Hassan, Ghezellou Parviz and Ghassempour Alireza, Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF, Current Proteomics 2015; 12 (1) . https://dx.doi.org/10.2174/157016461201150506201840
DOI https://dx.doi.org/10.2174/157016461201150506201840 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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