Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF

Title:Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF

Volume: 12 Issue: 1

Author(s): Saeedeh Mosafer Khorjestan, Behrooz Abtahi, Seyed Omid Ranei Siadat, Mohammad Motevalli, Hassan Rezadoost, Parviz Ghezellou and Alireza Ghassempour

Affiliation:

Keywords: Persian Gulf, phospholipase A2, sea snake, three-finger toxin, venom.

Abstract: Annulated sea snake, Hydrophis cyanocinctus, venom was investigated using reversed-phase high-performance liquid chromatography coupled to electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). MALDI-TOF/TOF identified three compounds (a short-chain neurotoxin, a long-chain neurotoxin and a basic phospholipase A₂); whose molecular weights were estimated to be 7285, 10838, and 14235 Da, respectively. Moreover, evolutionary relationship of each protein (identity more than 70%) was separately investigated via phylogenetic trees. This study was done as complementary investigation on the composition of the venom of H. cyanocinctus, the annulated sea snake from the Persian Gulf.

Cite this article as:

Mosafer Khorjestan Saeedeh, Abtahi Behrooz, Ranei Siadat Seyed Omid, Motevalli Mohammad, Rezadoost Hassan, Ghezellou Parviz and Ghassempour Alireza, Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF, Current Proteomics 2015; 12 (1) . https://dx.doi.org/10.2174/157016461201150506201840