Abstract
A genome-wide scanning of Sorghum bicolor resulted in the identification of 25 SbHsf genes. Phylogenetic analysis shows the ortholog genes that are clustered with only rice, representing a common ancestor. Promoter analysis revealed the identification of different cis-acting elements that are responsible for abiotic as well as biotic stresses. Hsf domains like DBD, NLS, NES, and AHA have been analyzed for their sequence similarity and functional characterization. Tissue specific expression patterns of Hsfs in different tissues like mature embryo, seedling, root, and panicle were studied using real-time PCR. While Hsfs4 and 22 are highly expressed in panicle, 4 and 9 are expressed in seedlings. Sorghum plants were exposed to different abiotic stress treatments but no expression of any Hsf was observed when seedlings were treated with ABA. High level expression of Hsf1 was noticed during high temperature as well as cold stresses, 4 and 6 during salt and 5, 6, 10, 13, 19, 23 and 25 during drought stress. This comprehensive analysis of SbHsf genes will provide an insight on how these genes are regulated in different tissues and also under different abiotic stresses and help to determine the functions of Hsfs during drought and temperature stress tolerance.
Keywords: Heat shock transcription factors, Phylogenetic analysis, Cis-acting elements, Paralogs, Molecular chaperones, Abiotic stress.
Graphical Abstract
Current Genomics
Title:Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors
Volume: 16 Issue: 4
Author(s): M. Nagaraju, Palakolanu Sudhakar Reddy, S. Anil Kumar, Rakesh K. Srivastava, P. B. Kavi Kishor and D. Manohar Rao
Affiliation:
Keywords: Heat shock transcription factors, Phylogenetic analysis, Cis-acting elements, Paralogs, Molecular chaperones, Abiotic stress.
Abstract: A genome-wide scanning of Sorghum bicolor resulted in the identification of 25 SbHsf genes. Phylogenetic analysis shows the ortholog genes that are clustered with only rice, representing a common ancestor. Promoter analysis revealed the identification of different cis-acting elements that are responsible for abiotic as well as biotic stresses. Hsf domains like DBD, NLS, NES, and AHA have been analyzed for their sequence similarity and functional characterization. Tissue specific expression patterns of Hsfs in different tissues like mature embryo, seedling, root, and panicle were studied using real-time PCR. While Hsfs4 and 22 are highly expressed in panicle, 4 and 9 are expressed in seedlings. Sorghum plants were exposed to different abiotic stress treatments but no expression of any Hsf was observed when seedlings were treated with ABA. High level expression of Hsf1 was noticed during high temperature as well as cold stresses, 4 and 6 during salt and 5, 6, 10, 13, 19, 23 and 25 during drought stress. This comprehensive analysis of SbHsf genes will provide an insight on how these genes are regulated in different tissues and also under different abiotic stresses and help to determine the functions of Hsfs during drought and temperature stress tolerance.
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Cite this article as:
Nagaraju M., Sudhakar Reddy Palakolanu, Anil Kumar S., K. Srivastava Rakesh, Kavi Kishor P. B. and Rao D. Manohar, Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors, Current Genomics 2015; 16 (4) . https://dx.doi.org/10.2174/1389202916666150313230812
DOI https://dx.doi.org/10.2174/1389202916666150313230812 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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