Physicochemical Characterization and Analgesic Effect of Inclusion Complexes of Essential Oil from Hyptis pectinata L. Poit Leaves with β-Cyclodextrin

Title:Physicochemical Characterization and Analgesic Effect of Inclusion Complexes of Essential Oil from Hyptis pectinata L. Poit Leaves with β-Cyclodextrin

Volume: 16 Issue: 5

Author(s): Paula dos P. Menezes, Adriano A. de S. Araujo, Grace Anne A. Doria, Lucindo J. Quintans-Junior, Makson G.B. de Oliveira, Marcio R.V. dos Santos, Juliana F. de Oliveira, Jivaldo do R. Matos, Flavio M. de S. Carvalho, Pericles B. Alves, Iara L. de Matos, Darlisson A. dos Santos, Ricardo N. Marreto, Gabriel F. da Silva and Mairim R. Serafini

Affiliation:

Keywords: β-cyclodextrin, (E)-caryophyllene, essential oil, Hyptis pectinata, inclusion complexes.

Abstract: The formation of inclusion complexes of Hyptis pectinata essential oil (EOHP), with potent activities such as anti-nociceptive, anti-inflammatory, among others, with β -cyclodextrin (β-CD), was obtained by slurry (SC) and paste procedures (PC). The gas chromatography coupled to the mass spectrometry (GC/MS) analysis demonstrated a total of 36.4% monoterpenes and 63.6% sesquiterpenes in the EOHP. The major components of EOHP were identified as (E)- caryophyllene (54.07%). The analysis of samples (PM, PC and SC) by GC/MS involved the surface and the total extracted oils. The GC/MS results suggested important differences between in SC and PC methods indicating the complexation of mono and sesquiterpenoids in different ratios. Furthermore, the thermal analysis techniques suggests the complexation, especially in SC, which show a thermogravimetry/derivative thermogravimetry (TG/DTG) peak at 140-270ºC, probably related to oil loss. Scanning electron microscopy (SEM) images showed reduction size of the samples mainly in the SC product. Additionally, EOHP/ β-CD improves pharmacological profile of EOHP alone in formalin-induced pain protocol in mice.

Cite this article as:

dos P. Menezes Paula, A. de S. Araujo Adriano, Anne A. Doria Grace, J. Quintans-Junior Lucindo, G.B. de Oliveira Makson, R.V. dos Santos Marcio, F. de Oliveira Juliana, do R. Matos Jivaldo, M. de S. Carvalho Flavio, B. Alves Pericles, L. de Matos Iara, A. dos Santos Darlisson, N. Marreto Ricardo, F. da Silva Gabriel and R. Serafini Mairim, Physicochemical Characterization and Analgesic Effect of Inclusion Complexes of Essential Oil from Hyptis pectinata L. Poit Leaves with β-Cyclodextrin, Current Pharmaceutical Biotechnology 2015; 16 (5) . https://dx.doi.org/10.2174/1389201015666141202101909