Abstract
This review focuses on analytical methods for the determination of reactive oxygen species (ROS): a superoxide/ hydroperoxyl radical, hydrogen peroxide, singlet oxygen and a hydroxyl radical. The methods which are described in this paper are based on: electron paramagnetic resonance spectroscopy (EPR), nuclear magnetic resonance (NMR), mass spectroscopy (MS), spectroscopy UV-Vis, gas chromatography (GC), chemiluminescence (CL), fluorescence (FL), HPLC –UV, iodide titration, and 14C-formate oxidation. In this review, the most common analytical methods for the detection of ROS generated by chemical methods are presented. The methods are based on the direct as well as indirect detection of ROS.
Keywords: Analytical methods, reactive oxygen species, radicals.
Graphical Abstract
Current Pharmaceutical Analysis
Title:Analytical Methods for Determination of Reactive Oxygen Species
Volume: 10 Issue: 4
Author(s): Joanna Pranczk, Dagmara Jacewicz, Dariusz Wyrzykowski and Lech Chmurzynski
Affiliation:
Keywords: Analytical methods, reactive oxygen species, radicals.
Abstract: This review focuses on analytical methods for the determination of reactive oxygen species (ROS): a superoxide/ hydroperoxyl radical, hydrogen peroxide, singlet oxygen and a hydroxyl radical. The methods which are described in this paper are based on: electron paramagnetic resonance spectroscopy (EPR), nuclear magnetic resonance (NMR), mass spectroscopy (MS), spectroscopy UV-Vis, gas chromatography (GC), chemiluminescence (CL), fluorescence (FL), HPLC –UV, iodide titration, and 14C-formate oxidation. In this review, the most common analytical methods for the detection of ROS generated by chemical methods are presented. The methods are based on the direct as well as indirect detection of ROS.
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Cite this article as:
Pranczk Joanna, Jacewicz Dagmara, Wyrzykowski Dariusz and Chmurzynski Lech, Analytical Methods for Determination of Reactive Oxygen Species, Current Pharmaceutical Analysis 2014; 10 (4) . https://dx.doi.org/10.2174/1573412910666140530221254
DOI https://dx.doi.org/10.2174/1573412910666140530221254 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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