Abstract
The L-amino acid oxidase (LAAO) is a multifunctional enzyme, able to partake in different activities including antibacterial activity. In this study, a novel LAAO (Mb-LAAO) was isolated from the venom of M. bornmuelleri snake using size exclusion chromatography followed by RP-HPLC and partially characterized. However, the molecular weight of the Mb-LAAO determined by ESI-MS and SDS-PAGE was 59 960.4 Da. Once the enzymatic activity test confirming the enzyme’s identity (transformation of L-leucine) was done, the Mb-LAAO was evaluated for its antibacterial activity against Gram-negative bacteria. It showed a remarkable effect against M. morganii and K. pneumoniae. Moreover, no cytotoxic activity was observed for Mb-LAAO against human erythrocytes arguing for an exploration of its pharmaceutical interest.
Keywords: Antibacterial effect, LAAO activity, L-amino acid oxidase, LC-ESI-MS analysis, Montivipera bornmuelleri.
Infectious Disorders - Drug Targets
Title:Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon
Volume: 13 Issue: 5
Author(s): Mohamad Rima, Claudine Accary, Katia Haddad, Riyad Sadek, Souad Hraoui-Bloquet, Jean C. Desfontis and Ziad Fajloun
Affiliation:
Keywords: Antibacterial effect, LAAO activity, L-amino acid oxidase, LC-ESI-MS analysis, Montivipera bornmuelleri.
Abstract: The L-amino acid oxidase (LAAO) is a multifunctional enzyme, able to partake in different activities including antibacterial activity. In this study, a novel LAAO (Mb-LAAO) was isolated from the venom of M. bornmuelleri snake using size exclusion chromatography followed by RP-HPLC and partially characterized. However, the molecular weight of the Mb-LAAO determined by ESI-MS and SDS-PAGE was 59 960.4 Da. Once the enzymatic activity test confirming the enzyme’s identity (transformation of L-leucine) was done, the Mb-LAAO was evaluated for its antibacterial activity against Gram-negative bacteria. It showed a remarkable effect against M. morganii and K. pneumoniae. Moreover, no cytotoxic activity was observed for Mb-LAAO against human erythrocytes arguing for an exploration of its pharmaceutical interest.
Export Options
About this article
Cite this article as:
Rima Mohamad, Accary Claudine, Haddad Katia, Sadek Riyad, Hraoui-Bloquet Souad, Desfontis C. Jean and Fajloun Ziad, Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon, Infectious Disorders - Drug Targets 2013; 13 (5) . https://dx.doi.org/10.2174/187152651305140403122334
DOI https://dx.doi.org/10.2174/187152651305140403122334 |
Print ISSN 1871-5265 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3989 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Canagliflozin : A New Hope in the Antidiabetic Armamentarium
Recent Patents on Cardiovascular Drug Discovery Pyrrolopyrazoles: Synthesis, Evaluation and Pharmacological Screening as Antidepressant Agents
Medicinal Chemistry Immunotherapeutic Options for Pediatric Malignancies
Current Immunology Reviews (Discontinued) Epidemiological and Clinical Data of Amniotic Fluid Embolism in Forensic Settings
Current Pharmaceutical Biotechnology The Renin-Angiotensin System: New Insight into Old Therapies
Current Medicinal Chemistry Retraction Notice: Current Review of Ramosetron in the Prevention of Postoperative Nausea and Vomiting
Current Drug Safety The Janus Face of Adenosine: Antiarrhythmic and Proarrhythmic Actions
Current Pharmaceutical Design Patents in the Diagnosis and Therapy of Neurocardiogenic Syncope
Recent Patents on Cardiovascular Drug Discovery An Efficient and Environmentally Benign Access Towards Synthesis of Novel 1,2,3-Triazolyl-pyrazoline Hybrids
Letters in Organic Chemistry Recent Advances in the Comprehension and the Management of Perioperative Systemic Host Response During Cardiopulmonary Bypass
Recent Patents on Cardiovascular Drug Discovery Adenosine in the Inflamed Gut: A Janus Faced Compound
Current Medicinal Chemistry New Perspectives on Acetaminophen
Current Cardiology Reviews Controversies in Glaucoma: Current Medical Treatment and Drug Development
Current Pharmaceutical Design Urotensin II: A Vascular Mediator in Health and Disease
Current Vascular Pharmacology Pharmacological Manipulation of Peripheral Vascular Resistance in Single Ventricle Patients (Stages I, II, and III of Palliation)
Current Vascular Pharmacology Role of the Sympathetic Nervous Activity in Hypertension-Update in 2013
Current Hypertension Reviews Ablation of TRPV1 Elevates Nocturnal Blood Pressure in Western Diet-fed Mice
Current Hypertension Reviews Recent Advancements in Diagnosis and Therapy of Liver Cirrhosis
Current Drug Targets Role of the 5-HT2C Receptor in Atypical Antipsychotics: Hero or Villain?
Current Medicinal Chemistry - Central Nervous System Agents Non-ambient Conditions in the Investigation and Manufacturing of Drug Forms
Current Pharmaceutical Design