Abstract
Countercurrent chromatography (CCC) is a unique liquid-liquid partition chromatography without any solid supports. In this paper, the progress for preparative separation of proteins and peptides by CCC has been reviewed. It covers the recent development of CCC instruments, including hydrostatic and hydrodynamic instruments, the optimization of two-phase solvent system, as well as the recent applications in separation of proteins and peptides. For biological purifications in CCC, the use of pH-zone-refining separation mode, and/or the use of aqueous two-phase systems, have been demonstrated to be the most effective protocols and exhibited potential possibility for sample pretreatments in proteomics studies.
Keywords: Aqueous two-phase systems, countercurrent chromatography, hydrostatic column, hydrodynamic column, peptides, pH-zone-refining, proteins.
Current Proteomics
Title:Separation of Peptides and Proteins by Countercurrent Chromatography
Volume: 10 Issue: 4
Author(s): Zhongming Xu, Xianjiang Lin and Yanbin Lu
Affiliation:
Keywords: Aqueous two-phase systems, countercurrent chromatography, hydrostatic column, hydrodynamic column, peptides, pH-zone-refining, proteins.
Abstract: Countercurrent chromatography (CCC) is a unique liquid-liquid partition chromatography without any solid supports. In this paper, the progress for preparative separation of proteins and peptides by CCC has been reviewed. It covers the recent development of CCC instruments, including hydrostatic and hydrodynamic instruments, the optimization of two-phase solvent system, as well as the recent applications in separation of proteins and peptides. For biological purifications in CCC, the use of pH-zone-refining separation mode, and/or the use of aqueous two-phase systems, have been demonstrated to be the most effective protocols and exhibited potential possibility for sample pretreatments in proteomics studies.
Export Options
About this article
Cite this article as:
Xu Zhongming, Lin Xianjiang and Lu Yanbin, Separation of Peptides and Proteins by Countercurrent Chromatography, Current Proteomics 2013; 10 (4) . https://dx.doi.org/10.2174/1570164610666131212000236
DOI https://dx.doi.org/10.2174/1570164610666131212000236 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Aftermath of Childhood Sexual Abuse: A Case Report and Review of the Literature
Adolescent Psychiatry Inflammatory Mechanisms and Oxidative Stress as Key Factors Responsible for Progression of Neurodegeneration: Role of Brain Innate Immune System
CNS & Neurological Disorders - Drug Targets Meet Our Editorial Board Member:
Current Alzheimer Research BX471: A CCR1 Antagonist with Anti-Inflammatory Activity in Man
Mini-Reviews in Medicinal Chemistry Novel Etomidate Derivatives
Current Pharmaceutical Design Intervention of Proliferation and Differentiation of Endogenous Neural Stem Cells in the Neurodegenerative Process of Huntingtons Disease Phenotype
CNS & Neurological Disorders - Drug Targets Nanocarbon Materials for Photodynamic Therapy and Photothermal Therapy
Pharmaceutical Nanotechnology Direct Modification of Bioactive Phenothiazines by Exposure to Laser Radiation
Recent Patents on Anti-Infective Drug Discovery Structure-Specific Ligand Recognition of Multistranded DNA Structures
Current Topics in Medicinal Chemistry Combining Structural Magnetic Resonance Imaging and Visuospatial Tests to Classify Mild Cognitive Impairment
Current Alzheimer Research Computational Insights into the Role of Glutathione in Oxidative Stress
Current Neurovascular Research Neuroprotection by Methylene Blue in Cerebral Global Ischemic Injury Induced Blood-Brain Barrier Disruption and Brain Pathology: A Review
CNS & Neurological Disorders - Drug Targets New Insights into Vitamin D and Autophagy in Inflammatory Bowel Diseases
Current Medicinal Chemistry Neuropathological Correlates of Cerebral Multimorbidity
Current Alzheimer Research Cyclooxygenase-2 and Prostaglandin E2 are Associated with Middle Cerebral Artery Occlusion and Hemorrhage in Patients with Moyamoya Disease
Current Neurovascular Research A Proteomic Analysis of Mitochondrial Complex III Inhibition in SH-SY5Y Human Neuroblastoma Cell Line
Current Proteomics Novel Directions in Antipsychotic Target Identification using Gene Arrays
Current Drug Targets - CNS & Neurological Disorders An Adolescent Psychiatrist Looks at the Story of Adolescent Addiction in The Basketball Diaries by Jim Carroll
Adolescent Psychiatry Type 2 Diabetes and Obesity in Children and Adolescents: Experience from Studies in Taiwanese Population
Current Diabetes Reviews Mitochondrial Permeability Transition Pore as a Suitable Targ e t for Neuroprotective Agents Against Alzheimer’s Disease
CNS & Neurological Disorders - Drug Targets