Sulfur-based Mechanistic Probes for Enzyme-catalyzed Reactions

Title:Sulfur-based Mechanistic Probes for Enzyme-catalyzed Reactions

Volume: 20 Issue: 30

Author(s): Weiping Zheng

Affiliation:

Keywords: Sulfur-based, mechanistic probes, enzymatic reactions.

Abstract: Sulfur (S), as the second element in the main group 6A just below oxygen (O), has been often used as an isosteric replacement for O in enzymatic mechanistic studies. In addition, S has also been used as an isosteric replacement for CH₂. These S-based mechanistic probes have been used in the studies with protein enzyme systems such as the sirtuin family of the protein N^ε-acyl-lysine deacylases, phosphotransferases, and fatty acid desaturase, as well as various RNA enzymes (ribozymes). These probes are basically the O→S mutants of the corresponding O(or CH₂)-containing substrates for the enzymatic reactions under study. This article will review the significant contributions that the S-based probes have been able to make toward an enhanced mechanistic understanding of different types of the enzyme-catalyzed reactions.

Cite this article as:

Zheng Weiping, Sulfur-based Mechanistic Probes for Enzyme-catalyzed Reactions, Current Medicinal Chemistry 2013; 20 (30) . https://dx.doi.org/10.2174/09298673113209990177