Identification of an Amyloidogenic Peptide From the Bap Protein of Staphylococcus epidermidis

Title:Identification of an Amyloidogenic Peptide From the Bap Protein of Staphylococcus epidermidis

Volume: 21 Issue: 1

Author(s): Pierre Lembré, Charlotte Vendrely and Patrick Di Martino

Affiliation:

Keywords: Amyloid, bap, biofilm, peptide, Staphylococcus epidermidis.

Abstract: Biofilm associated proteins (Bap) are involved in the biofilm formation process of several bacterial species. The sequence STVTVT is present in Bap proteins expressed by many Staphylococcus species, Acinetobacter baumanii and Salmonella enterica. The peptide STVTVTF derived from the C-repeat of the Bap protein from Staphylococcus epidermidis was selected through the AGGRESCAN, PASTA, and TANGO software prediction of protein aggregation and formation of amyloid fibers. We characterized the self-assembly properties of the peptide STVTVTF by different methods: in the presence of the peptide, we observed an increase in the fluorescence intensity of Thioflavin T; many intermolecular β-sheets and fibers were spontaneously formed in peptide preparations as observed by infrared spectroscopy and atomic force microscopy analyses. In conclusion, a 7 amino acids peptide derived from the C-repeat of the Bap protein was sufficient for the spontaneous formation of amyloid fibers. The possible involvement of this amyloidogenic sequence in protein-protein interactions is discussed.

Cite this article as:

Lembré Pierre, Vendrely Charlotte and Martino Di Patrick, Identification of an Amyloidogenic Peptide From the Bap Protein of Staphylococcus epidermidis, Protein & Peptide Letters 2014; 21 (1) . https://dx.doi.org/10.2174/09298665113209990072