Abstract
The development of imaging probes based on advances in nanotechnology aims to substantially improve specificity and sensitivity of diagnostic imaging through non-invasive and quantitative detection of specific biomolecules in living subjects. A promising class of molecular imaging probes consists of nanoparticles (NPs) functionalized with a certain targeting agent. Such targeting agents can, for instance, be selected to recognize disease-related biomarkers located on the cell surface. Among the possible agents that direct the NPs to the target site, aptamers, being single-stranded DNA or RNA molecules that can be designed to bind preselected targets such as proteins and peptides with high affinity and specificity, play an increasingly important role. Indeed, aptamers selected against proteins or whole cells have been conjugated to a variety of nanomaterials (NMs) such as Au NPs, quantum dots (QD), and superparamagnetic iron oxide NPs (SPIONs). These probes have successfully been used for cell imaging, both in vitro and in vivo, by optical imaging, magnetic resonance imaging (MRI), computed tomography (CT), and positron-emission tomography (PET). This review presents an overview of the commonly used techniques involved in conjugating aptamer to NPs and their application as probes in cellular or in vivo imaging.
Keywords: Aptamer, aptamer-conjugated nanoparticles, conjugation, medical imaging, diagnosis, nanoparticles
Current Topics in Medicinal Chemistry
Title:Conjugation Approaches for Construction of Aptamer-Modified Nanoparticles for Application in Imaging
Volume: 13 Issue: 4
Author(s): Jung Hwan Lee
Affiliation:
Keywords: Aptamer, aptamer-conjugated nanoparticles, conjugation, medical imaging, diagnosis, nanoparticles
Abstract: The development of imaging probes based on advances in nanotechnology aims to substantially improve specificity and sensitivity of diagnostic imaging through non-invasive and quantitative detection of specific biomolecules in living subjects. A promising class of molecular imaging probes consists of nanoparticles (NPs) functionalized with a certain targeting agent. Such targeting agents can, for instance, be selected to recognize disease-related biomarkers located on the cell surface. Among the possible agents that direct the NPs to the target site, aptamers, being single-stranded DNA or RNA molecules that can be designed to bind preselected targets such as proteins and peptides with high affinity and specificity, play an increasingly important role. Indeed, aptamers selected against proteins or whole cells have been conjugated to a variety of nanomaterials (NMs) such as Au NPs, quantum dots (QD), and superparamagnetic iron oxide NPs (SPIONs). These probes have successfully been used for cell imaging, both in vitro and in vivo, by optical imaging, magnetic resonance imaging (MRI), computed tomography (CT), and positron-emission tomography (PET). This review presents an overview of the commonly used techniques involved in conjugating aptamer to NPs and their application as probes in cellular or in vivo imaging.
Export Options
About this article
Cite this article as:
Lee Jung Hwan, Conjugation Approaches for Construction of Aptamer-Modified Nanoparticles for Application in Imaging, Current Topics in Medicinal Chemistry 2013; 13 (4) . https://dx.doi.org/10.2174/1568026611313040010
DOI https://dx.doi.org/10.2174/1568026611313040010 |
Print ISSN 1568-0266 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4294 |
Call for Papers in Thematic Issues
Medicinal Chemistry Advancement in Life-Threatening Diseases
The current issue will highlight concise reports that specify ground-breaking insights, including the novel discovery of drug targets and their action mechanism or drugs of novel classes. These are projected to encourage medicinal chemistry future efforts to address the most challenging medical needs. The current issue highlights further efforts to ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Recent Advances in the Development of Selective Ligands for the Cannabinoid CB2 Receptor
Current Topics in Medicinal Chemistry Base Excision Repair: Contribution to Tumorigenesis and Target in Anticancer Treatment Paradigms
Current Medicinal Chemistry Brain Aging and Disorders of the Central Nervous System: Kynurenines and Drug Metabolism
Current Drug Metabolism Stationary Wavelet Transform and AdaBoost with SVM Based Pathological Brain Detection in MRI Scanning
CNS & Neurological Disorders - Drug Targets Disintegrins from Snake Venoms and their Applications in Cancer Research and Therapy
Current Protein & Peptide Science Natural Products as Exquisitely Potent Cytotoxic Payloads for Antibody- Drug Conjugates
Current Topics in Medicinal Chemistry Magnetic Hyperthermia with Magnetic Nanoparticles: A Status Review
Current Topics in Medicinal Chemistry MMPs in Ovarian Cancer as Therapeutic Targets
Anti-Cancer Agents in Medicinal Chemistry ErbB Antagonists Patenting: “Playing Chess with Cancer”
Recent Patents on Biotechnology Is Fibroblast Growth Factor Receptor 4 a Suitable Target of Cancer Therapy?
Current Pharmaceutical Design MicroRNAs in Prostate Cancer Following Radiotherapy: Towards Predicting Response to Radiation Treatment
Current Medicinal Chemistry The Urokinase-type Plasminogen Activator and the Generation of Inhibitors of Urokinase Activity and Signaling
Current Pharmaceutical Design Clinical Trials with Oncolytic Measles Virus: Current Status and Future Prospects
Current Cancer Drug Targets Membrane Targeted Anticancer Drugs: Potent Inducers of Apoptosis and Putative Radiosensitisers
Current Medicinal Chemistry - Anti-Cancer Agents Macroautophagy as a Target of Cancer Therapy
Current Cancer Therapy Reviews Evaluation of Melatonin Effect on Human Breast Cancer Stem Cells Using a Threedimensional Growth Method of Mammospheres
Anti-Cancer Agents in Medicinal Chemistry The Role of Connexins in Carcinogenesis: Review of Current Knowledge
Current Signal Transduction Therapy Matrix Metalloproteinases as Potential Targets in the Venous Dilation Associated with Varicose Veins
Current Drug Targets Recent Advances of Natural and Synthetic β-Carbolines as Anticancer Agents
Anti-Cancer Agents in Medicinal Chemistry NSAIDs and Chemoprevention
Current Cancer Drug Targets