L-arginine Mediated Renaturation Enhances Yield of Human, α6 Type IV Collagen Non-collagenous Domain from Bacterial Inclusion Bodies

Venugopal      Gunda; Chandra      Shekhar Boosani; Raj      Kumar Verma; Chittibabu      Guda; Yakkanti      Akul Sudhakar

doi:10.2174/092986612802762750

Abstract

The anti-angiogenic, carboxy terminal non-collagenous domain (NC1) derived from human Collagen type IV alpha 6 chain, [α6(IV)NC1] or hexastatin, was earlier obtained using different recombinant methods of expression in bacterial systems. However, the effect of L-arginine mediated renaturation in enhancing the relative yields of this protein from bacterial inclusion bodies has not been evaluated. In the present study, direct stirring and on-column renaturation methods using L-arginine and different size exclusion chromatography matrices were applied for enhancing the solubility in purifying the recombinant α6(IV)NC1 from bacterial inclusion bodies. This methodology enabled purification of higher quantities of soluble protein from inclusion bodies, which inhibited endothelial cell proliferation, migration and tube formation. Thus, the scope for L-arginine mediated renaturation in obtaining higher yields of soluble, biologically active NC1 domain from bacterial inclusion bodies was evaluated.

Keywords: Non-collagenous domain of α6 type IV collagen, L-arginine mediated renaturation, size exclusion chromatography, anti-angiogenic activity, human umbilical vein endothelial cells and vascular endothelial growth factor, inclusion bodies, blood capillaries

« Previous Next »