Abstract
Detection of enzymatic activity on gel electrophoresis, namely zymography, is a technique that has received increasing attention in the last 10 years, according to the number of articles published. A growing amount of enzymes, mainly proteases, are now routinely detected by zymography. Detailed analytical studies are beginning to be published, as well as new patents have been developed. This new article updates the information covered in our last review, condensing the recent publications dealing with the identification of proteolytic enzymes in electrophoretic gel supports and its variations. The new advances of this method are basically focused towards two dimensional zymography and transfer zymography. Though comparatively fewer patents have been published, they basically coincide in the study of matrix metalloproteases. The tendency is foreseen to be very productive in the area of zymoproteomics, combining electrophoresis and mass spectrometry for the analysis of proteases.
Keywords: Electrophoresis, enzymes, in-situ-zymography, matrix metalloproteases, proteases, reverse zymography, transfer zymography, two-dimensional zymography, zymography, zymoproteomics, native PAGE, cathepsin zymography, IEF, MMP, NMDA
Recent Patents on Biotechnology
Title:Advances in Zymography Techniques and Patents Regarding Protease Analysis
Volume: 6 Issue: 2
Author(s): Jeff Wilkesman and Liliana Kurz
Affiliation:
Keywords: Electrophoresis, enzymes, in-situ-zymography, matrix metalloproteases, proteases, reverse zymography, transfer zymography, two-dimensional zymography, zymography, zymoproteomics, native PAGE, cathepsin zymography, IEF, MMP, NMDA
Abstract: Detection of enzymatic activity on gel electrophoresis, namely zymography, is a technique that has received increasing attention in the last 10 years, according to the number of articles published. A growing amount of enzymes, mainly proteases, are now routinely detected by zymography. Detailed analytical studies are beginning to be published, as well as new patents have been developed. This new article updates the information covered in our last review, condensing the recent publications dealing with the identification of proteolytic enzymes in electrophoretic gel supports and its variations. The new advances of this method are basically focused towards two dimensional zymography and transfer zymography. Though comparatively fewer patents have been published, they basically coincide in the study of matrix metalloproteases. The tendency is foreseen to be very productive in the area of zymoproteomics, combining electrophoresis and mass spectrometry for the analysis of proteases.
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Cite this article as:
Wilkesman Jeff and Kurz Liliana, Advances in Zymography Techniques and Patents Regarding Protease Analysis, Recent Patents on Biotechnology 2012; 6 (2) . https://dx.doi.org/10.2174/187220812801784678
DOI https://dx.doi.org/10.2174/187220812801784678 |
Print ISSN 1872-2083 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-4012 |
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