Abstract
Eukaryotic gene expression is tightly controlled by a cascade of regulatory mechanisms. At the sequence level, gene expression is regulated by cis-acting DNA motifs that are able to recruit trans-acting transcription factors (TFs) for positive or negative regulation of local gene expression. The genome-wide mapping of transcription factor binding sites (TFBS) becomes a crucial strategy for the study of gene expression regulation. Here in this chapter we will discuss the preparation of ChIP-TFBS sequencing libraries and the analysis of ChIP-TFBS sequence data.
Keywords: ChIP, ChIP-TFBS, Chromatin immunoprecipitation, Motif, TF, Transcription factor.
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