Micropropagation of Medicinal Plants

Volume: 2

In Vitro Protocols for Micropropagation of Catharanthus roseus (L.) G. Don

Author(s): Govindugari Vijaya Laxmi* and K. Dharmalingam

Pp: 170-187 (18)

DOI: 10.2174/9789815238303124020012

* (Excluding Mailing and Handling)

Abstract

Catharanthus roseus (C. roseus) is an important alkaloid-yielding medicinal and ornamental plant belonging to the family Apocynaceae. The genus Catharanthus is well studied and reported to contain biologically active terpenoid indole alkaloids (TIAs) with over 130 compounds isolated and identified. It has great medicinal importance in treating various ailments to treat diseases as diabetes, malaria, menorrhagia, Hodgkin’s disease, etc. In view of the immense importance in the pharmaceutical industry, micropropagation of C. roseus has been the best alternative for continuous source of plants and also for in vitro production of secondary metabolites. Various explants have been studied for micropropagation; however, nodal explants were the most suitable. For surface sterilization, 0.1% HgCl2 or 70% ethanol, followed by sodium hypochlorite and Bavistin (carbendazim), was optimum to control the microbial contamination. Murashige and Skoog (MS) medium was the most widely used for its success rate. 2,4-D for callus initiation and BAP, along with zeatin and activated charcoal, were reported to be promising for regeneration of plantlets. The 100% acclimatization of plantlets on transfer to field depends on the soil mixture and environmental conditions and humidity in the initial stages of transfer from in vitro cultures.

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      • 11. app/Models/ContentAccess.php:647
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      • 11. app/Models/ContentAccess.php:647
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      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
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      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1518
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316548017 and j.to_ip >=316548017 )) and ((j.from_date <= 1740776058 and j.to_date >= 1740776058 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 21895) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316548017 and j.to_ip >=316548017 )) and ((j.from_date <= 1740776058 and j.to_date >= 1740776058 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 21895) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316548017 and j.to_ip >=316548017 )) and ((j.from_date <= 1740776058 and j.to_date >= 1740776058 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 21895)
      1.39msalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1549
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` where `b`.`banner_ebook` = 1 and `bd`.`content_type` = 'B' and `bd`.`content_id` = 3688 and `b`.`banner_from_date` <= 1740776058 and `b`.`banner_to_date` >= 1740776058 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.94msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: B
      • 2: 3688
      • 3: 1740776058
      • 4: 1740776058
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select count(*) as aggregate from `uc_cart_products` where cart_id='a3c9e3d93244759b2fb6c87ddfe90f88'
      17.51msalphaeurekaselec_live_10_06_2022Cart.php#271
      Backtrace
      • 15. app/Models/Cart.php:271
      • 16. view::layouts._header:291
      • 18. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 20. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    App\Models\Chapter
    1Chapter.php#?
    App\Models\Ebook
    1Ebook.php#?
        _token
        hhI6X3I0rhjqRGf0BNgp7uyGJBFoFFVW5CqjHD8Z
        uc_cart_id
        a3c9e3d93244759b2fb6c87ddfe90f88
        _previous
        array:1 [ "url" => "http://alpha.eurekaselect.com/chapter/21895" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /chapter/21895
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
        array:8 [ "accept-encoding" => array:1 [ 0 => "gzip, deflate" ] "accept" => array:1 [ 0 => "text/html,application/xhtml+xml,application/xml;q=0.9,image/avif,image/webp,image/apng,*/*;q=0.8,application/signed-exchange;v=b3;q=0.7" ] "user-agent" => array:1 [ 0 => "Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)" ] "upgrade-insecure-requests" => array:1 [ 0 => "1" ] "cache-control" => array:1 [ 0 => "no-cache" ] "pragma" => array:1 [ 0 => "no-cache" ] "connection" => array:1 [ 0 => "keep-alive" ] "host" => array:1 [ 0 => "alpha.eurekaselect.com" ] ]
        request_cookies
        []
        
        response_headers
        0 of 0
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        session_attributes
        0 of 0
        array:5 [ "_token" => "hhI6X3I0rhjqRGf0BNgp7uyGJBFoFFVW5CqjHD8Z" "uc_cart_id" => "a3c9e3d93244759b2fb6c87ddfe90f88" "_previous" => array:1 [ "url" => "http://alpha.eurekaselect.com/chapter/21895" ] "_flash" => array:2 [ "old" => [] "new" => [] ] "PHPDEBUGBAR_STACK_DATA" => [] ]
        ClearShow all
        Date ↕MethodURLData
        #12025-02-28 20:54:18GET/chapter/21895112192