Abstract
Tylophora indica (Burm.f.) Merrill is one of the most commonly used
medicinal plants with bioactive alkaloid-rich secondary metabolites. This plant is used
to treat asthma, dysentery, whooping cough, rheumatic pains, jaundice, and cancer.
Rapid exploitation of this plant in natural habitats and poor regeneration methods,
which are not in pace with those of destruction, make tissue culture methods a viable
option to be used as a method of conservation. In the present chapter, tissue culture
protocols have been reported till now as the best viable means in the rapid
multiplication of T. indica. Sterilization protocols, callus induction and somatic
embryogenesis methods, and direct and indirect organogenesis used by different
researchers in mass propagation and acclimatization are given in detail. The present
chapter gives an insight into the hormones needed and the response of the explants,
which will be helpful for those who want to propagate this medicinal plant under in
vitro conditions.