Abstract
Apoptosis is a critical process having widespread biological significance; regulating the differentiation, proliferation, maintenance and sculpturing organs and tissues, functioning of immune system and the elimination of defective harmful cells. The word apoptosis is derived from Greek word meaning “απόπτωσις” meaning “falling off of petals from flowers”. It is actually programmed cell death essential for normal metabolism. In this chapter, we will provide general overview of various technical approaches for detecting apoptotic cells. The features, advantages and disadvantages of different methods are also discussed.
Keywords: Annexin, Caspases, DNA polymerase, Hoechst stain, Homeostasis, Luminometry, Membrane-blebbing, Necrosis, Phosphatidyl-serine, Propidium iodide, TUNEL assay.
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