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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

Sam68通过诱导EphA3激活来促进人类乳腺癌的发展

卷 20, 期 1, 2020

页: [76 - 83] 页: 8

弟呕挨: 10.2174/1568009619666190718124541

价格: $65

摘要

背景：与68 kDa有丝分裂相关的Src（Sam68）通常在人类癌症中高表达。在某些癌症患者中，Sam68的过度表达与生存预后不良有关。但是，尚不清楚Sam68是否在促进乳腺癌转移中起作用。 材料与方法：采用免疫组织化学方法检测Sam68蛋白在乳腺癌组织中的表达。采用Trans-well测定，伤口愈合，实时PCR和Western blotting分析来检测Sam68对促进EMT或转移乳腺癌的作用。下一代RNA测序用于分析可能受Sam68调控的基因。 结果：Sam68在促进乳腺癌转移中起积极作用。 Sam68被发现在乳腺癌以及淋巴结转移中过表达。还发现MMP-9在乳腺癌组织中过表达，并且与Sam68的表达相关（P <0.01）。 NOD / SCID小鼠的异种移植和体外实验证实，Sam68调节乳腺癌细胞的侵袭和转移能力。 Sam68可能在乳腺癌中上调EPHA3。 结论：Sam68的高表达通过上调EPHA3基因参与乳腺癌的转移。

关键词: Sam68，乳腺癌，EPHA3，转移，免疫组化，异种移植。

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