Abstract
Background: The present investigation aimed to evaluate the role of Plant Growth- Promoting Rhizobacteria (PGPR) and Ag-nanoparticles on two varieties (American variety, Poinsett 76 and Desi variety, Sialkot selection) of cucumber plants.
Methods: Cucumber seeds prior to sowing, were inoculated with two strains of PGPR, Pseudomonas putida (KX574857) and Pseudomonas stutzeri (KX574858) at the rate of 106 cells/ml. Agnanoparticles (5ppm) were sprayed on the plant at early vegetative phase 27 d after sowing.
Results: The proline, sugar, protein, phenolics, flavonoids, chlorophyll and carotenoids contents of leaves of plants and the activities of Phenylalanine Ammonia-Lyase (PAL), Superoxide Dismutase (SOD) and Catalase (CAT) were determined from leaves of plants at early vegetative phase. After 3 months of seeds sowing, Ag-nanoparticles enhanced the length of root but decreased the length of shoot and fresh weight of root and shoot as compared to control whereas, the leaf protein, proline, phenolics, flavonoids, chlorophyll b, total chlorophyll, sugar and Phenylalanine Ammonia-Lyase (PAL) activity of plants were increased significantly over control. Ag-nanoparticles also suppressed the effect of PGPR for root, shoot length but augmented the protein and phenolics contents of leaves of both the varieties.
Conclusion: The combined treatment of Ag-nanoparticles and PGPR enhanced flavonoids content of leaves and the activities of PAL, SOD and CAT in leaves of plants over control. Agnanoparticles effectively increased the Phenylalanine Ammonia-Lyase (PAL), Catalase (CAT) and superoxide dismutase (SOD) activities in leaves of both the varieties. Pseudomonas putida may be used either alone or in combination with Ag-nanoparticles to enhance the antioxidant and defense enzyme activities. Hence, the plant can tolerate the diseases and stresses in a much better way with higher protein and phenolics content.
Keywords: Ag-nanoparticles, PGPR, cucumber, antioxidant enzymes, PAL, carotenoids.
Graphical Abstract
[1]
Rahman AH, Anisuzzaman M, Ahmed F, Islam AK, Naderuzzaman AT. Study of nutritive value and medicinal uses of cultivated cucurbits. J Appl Sci Res 2008; 4(5): 555-8.
[2]
Naseem H, Ahsan M, Shahid MA, Khan N. Exopolysaccharides producing rhizobacteria and their role in plant growth and drought tolerance. J Basic Microbiol 2018; 58(12): 1009-22.
[http://dx.doi.org/10.1002/jobm.201800309]
[http://dx.doi.org/10.1002/jobm.201800309]
[3]
Chen X, Schluesener HJ. Nanosilver: a nanoproduct in medical application. Toxicol Lett 2008; 176(1): 1-12.
[http://dx.doi.org/10.1016/j.toxlet.2007.10.004] [PMID: 18022772]
[http://dx.doi.org/10.1016/j.toxlet.2007.10.004] [PMID: 18022772]
[4]
Khan N, Bano A, Rahman MA, Guo J, Kang Z, Babar MA. Comparative physiological and metabolic analysis reveals a complex mechanism involved in drought tolerance in chickpea (Cicer arietinum L.) induced by PGPR and PGRs. Sci Rep 2019; 9(1): 2097.
[http://dx.doi.org/10.1038/s41598-019-38702-8]
[http://dx.doi.org/10.1038/s41598-019-38702-8]
[5]
Shams G, Ranjbar M, Amiri A. Effect of silver nanoparticles on concentration of silver heavy element and growth indexes in cucumber (Cucumis sativus L. negeen). J Nanopart Res 2013; 15(5): 1630.
[6]
Thuesombat P, Hannongbua S, Akasit S, Chadchawan S. Effect of silver nanoparticles on rice (Oryza sativa L. cv. KDML 105) seed germination and seedling growth. Ecotoxicol Environ Saf 2014; 104: 302-9.
[http://dx.doi.org/10.1016/j.ecoenv.2014.03.022] [PMID: 24726943]
[http://dx.doi.org/10.1016/j.ecoenv.2014.03.022] [PMID: 24726943]
[7]
Khan N, Bano A, Zandi P. Effects of exogenously applied plant growth regulators in combination with PGPR on the physiology and root growth of chickpea (Cicer arietinum) and their role in drought tolerance. J Plant Interact 2018; 13(1): 239-47.
[http://dx.doi.org/10.1080/17429145.2018.1471527]
[http://dx.doi.org/10.1080/17429145.2018.1471527]
[8]
Abdel-Aziz MS, Shaheen MS, El-Nekeety AA, Abdel-Wahhab MA. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. J Saudi Chem Soc 2014; 18(4): 356-63.
[http://dx.doi.org/10.1016/j.jscs.2013.09.011]
[http://dx.doi.org/10.1016/j.jscs.2013.09.011]
[9]
Adesemoye AO, Egamberdieva D. Beneficial effects of plant growth-promoting rhizobacteria on improved crop production: prospects for developing economiesBacteria in Agrobiology: Crop productivity. Berlin, Heidelberg: Springer 2013; pp. 45-63.
[http://dx.doi.org/10.1007/978-3-642-37241-4_2]
[http://dx.doi.org/10.1007/978-3-642-37241-4_2]
[10]
Alscher RG, Erturk N, Heath LS. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 2002; 53(372): 1331-41.
[http://dx.doi.org/10.1093/jexbot/53.372.1331] [PMID: 11997379]
[http://dx.doi.org/10.1093/jexbot/53.372.1331] [PMID: 11997379]
[11]
Khan N, Bano A, Babar MA. Metabolic and physiological changes induced by plant growth regulators and plant growth promoting rhizobacteria and their impact on drought tolerance in Cicer arietinum L. PLoS One 2019; 14(3): e0213040
[http://dx.doi.org/10.1371/journal.pone.0213040]
[http://dx.doi.org/10.1371/journal.pone.0213040]
[12]
Bertrand H, Plassard C, Pinochet X, Touraine B, Normand P, Cleyet-Marel JC. Stimulation of the ionic transport system in Brassica napus by a plant growth-promoting rhizobacterium (Achromobacter sp.). Can J Microbiol 2000; 46(3): 229-36.
[http://dx.doi.org/10.1139/w99-137] [PMID: 10749536]
[http://dx.doi.org/10.1139/w99-137] [PMID: 10749536]
[13]
Bharathi S. Developing botanical formulations for the management of major fungal diseases of tomato and onion 2004.
[14]
Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microbiol Biotechnol 2012; 28(4): 1327-50.
[http://dx.doi.org/10.1007/s11274-011-0979-9] [PMID: 22805914]
[http://dx.doi.org/10.1007/s11274-011-0979-9] [PMID: 22805914]
[15]
Chen C, Belanger RR, Benhamou N, Paulitz TC. Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiol Mol Plant Pathol 2000; 56(1): 13-23.
[http://dx.doi.org/10.1006/pmpp.1999.0243]
[http://dx.doi.org/10.1006/pmpp.1999.0243]
[16]
del Río LA, Corpas FJ, Sandalio LM, Palma JM, Barroso JB. Plant peroxisomes, reactive oxygen metabolism and nitric oxide. IUBMB Life 2003; 55(2): 71-81.
[http://dx.doi.org/10.1002/tbmb.718540875] [PMID: 12749689]
[http://dx.doi.org/10.1002/tbmb.718540875] [PMID: 12749689]
[17]
Hussain ST, Mehmood T. Antifungal activity of silver nanoparticles. International conference 2011.Organized by National Centre for physics. .
[18]
Khan N, Zandi P, Ali S, Mehmood A, Shahid MA. Impact of Salicylic acid and PGPR on the Drought Tolerance and Phytoremediation potential of Helianthus annus. Front Microbiol 2018; 9: 2507.
[http://dx.doi.org/10.3389/fmicb.2018.02507]
[http://dx.doi.org/10.3389/fmicb.2018.02507]
[19]
Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28(3): 350-6.
[http://dx.doi.org/10.1021/ac60111a017]
[http://dx.doi.org/10.1021/ac60111a017]
[20]
Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil 1973; 39(1): 205-7.
[http://dx.doi.org/10.1007/BF00018060]
[http://dx.doi.org/10.1007/BF00018060]
[21]
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64(4): 555-9.
[http://dx.doi.org/10.1016/S0308-8146(98)00102-2]
[http://dx.doi.org/10.1016/S0308-8146(98)00102-2]
[22]
Gholami A, Shahsavani S, Nezarat S. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. International Journal of Biology Life Science 2009; 5(1): 35-40.
[23]
Suzuki LS, Herrig V, Ferrarese ML, Rodrigues JD, Ferrarese-Filho O. Simultaneous effects of ferulic and vanillic acids on peroxidase and phenylalanine ammonia-lyase in soybean (Glycine max) roots. Phyton 2003; 43(1): 179-85.
[24]
Dos Santos WD, Ferrarese Mde L, Finger A, Teixeira AC, Ferrarese-Filho O. Lignification and related enzymes in Glycine max root growth-inhibition by ferulic acid. J Chem Ecol 2004; 30(6): 1203-12.
[25]
Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971; 44(1): 276-87.
[http://dx.doi.org/10.1016/0003-2697(71)90370-8] [PMID: 4943714]
[http://dx.doi.org/10.1016/0003-2697(71)90370-8] [PMID: 4943714]
[26]
Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res 2010; 4(7): 191-5.
[27]
Gruyer N, Dorais M, Bastien C, Dassylva N, Triffault-Bouchet G. Interaction between silver nanoparticles and plant growth. International
Symposium on New Technologies for Environment Control,
Energy-Saving and Crop Production in Greenhouse and Plant 1037. 795-800.
[28]
Hirschberg J. Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol 2001; 4(3): 210-8.
[http://dx.doi.org/10.1016/S1369-5266(00)00163-1] [PMID: 11312131]
[http://dx.doi.org/10.1016/S1369-5266(00)00163-1] [PMID: 11312131]
[29]
Johnson RR, Balwani TL, Johnson LJ, McClure KE, Dehority BA. Corn Plant Maturity. II. Effect on in vitro cellulose digestibility and soluble carbohydrate content 1. J Anim Sci 1966; 25(3): 617-23.
[http://dx.doi.org/10.2527/jas1966.253617x] [PMID: 5338818]
[http://dx.doi.org/10.2527/jas1966.253617x] [PMID: 5338818]
[30]
Krishnaraj C, Jagan EG, Ramachandran R, Abirami SM, Mohan N, Kalaichelvan PT. Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. plant growth metabolism. Process Biochem 2012; 47(4): 651-8.
[http://dx.doi.org/10.1016/j.procbio.2012.01.006]
[http://dx.doi.org/10.1016/j.procbio.2012.01.006]
[31]
Lamsal K, Kim SW, Jung JH, Kim YS, Kim KS, Lee YS. Inhibition effects of silver nanoparticles against powdery mildews on cucumber and pumpkin. Mycobiology 2011; 39(1): 26-32.
[http://dx.doi.org/10.4489/MYCO.2011.39.1.026] [PMID: 22783069]
[http://dx.doi.org/10.4489/MYCO.2011.39.1.026] [PMID: 22783069]
[32]
Lewis RW, Unrine J, Bertsch PM, McNear DH Jr. Silver engineered nanomaterials and ions elicit species-specific O2 consumption responses in plant growth promoting rhizobacteria. Biointerphases 12(5)2017;
[http://dx.doi.org/10.1116/1.4995605]
[http://dx.doi.org/10.1116/1.4995605]
[33]
MacDonald MJ, D’Cunha GB. A modern view of phenylalanine ammonia lyase. Biochem Cell Biol 2007; 85(3): 273-82.
[http://dx.doi.org/10.1139/O07-018] [PMID: 17612622]
[http://dx.doi.org/10.1139/O07-018] [PMID: 17612622]
[34]
Khan N, Bano A. Modulation of phytoremediation and plant growth by the treatment with PGPR, Ag nanoparticle and untreated municipal wastewater. Int J Phytoremediation 2016; 18(12): 1258-69.
[http://dx.doi.org/10.1080/15226514.2015.1064352]
[http://dx.doi.org/10.1080/15226514.2015.1064352]
[35]
Khan N, Bano A, Rahman MA, Rathinasabapathi B, Babar MA. UPLC‐HRMS‐based untargeted metabolic profiling reveals changes in chickpea (Cicer arietinum) metabolome following long‐term drought stress. Plant Cell Environ 2019; 42(1): 115-32.
[http://dx.doi.org/10.1111/pce.13195]
[http://dx.doi.org/10.1111/pce.13195]
[36]
Mantelin S, Touraine B. Plant growth-promoting bacteria and nitrate availability: impacts on root development and nitrate uptake. J Exp Bot 2004; 55(394): 27-34.
[http://dx.doi.org/10.1093/jxb/erh010] [PMID: 14623902]
[http://dx.doi.org/10.1093/jxb/erh010] [PMID: 14623902]
[37]
Marulanda A, Barea JM, Azcón R. Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. J Plant Growth Regul 2009; 28(2): 115-24.
[http://dx.doi.org/10.1007/s00344-009-9079-6]
[http://dx.doi.org/10.1007/s00344-009-9079-6]
[38]
Matysik J. Alia, Bhalu B, Mohanty P. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr Sci 2002; 10: 525-32.
[39]
Minorsky PV. On the inside. Plant Physiol 2008; 146(4): 1455-6.
[http://dx.doi.org/10.1104/pp.104.900255]
[http://dx.doi.org/10.1104/pp.104.900255]
[40]
Mukherjee PK, Nema NK, Maity N, Sarkar BK. Phytochemical and therapeutic potential of cucumber. Fitoterapia 2013; 84: 227-36.
[http://dx.doi.org/10.1016/j.fitote.2012.10.003] [PMID: 23098877]
[http://dx.doi.org/10.1016/j.fitote.2012.10.003] [PMID: 23098877]
[41]
Khan N, Bano A. Role of plant growth promoting rhizobacteria and Ag-nano particle in the bioremediation of heavy metals and maize growth under municipal wastewater irrigation. Int J Phytoremediation 2016; 18(3): 211-21.
[http://dx.doi.org/10.1080/15226514.2015.1064352]
[http://dx.doi.org/10.1080/15226514.2015.1064352]
[42]
Salama HM. Effects of silver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.). Int Res J Biotechnol 2012; 3(10): 190-7.
[44]
Santoyo G, Orozco-Mosqueda MD, Govindappa M. Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review. Biocontrol Sci Technol 2012; 22(8): 855-72.
[http://dx.doi.org/10.1080/09583157.2012.694413]
[http://dx.doi.org/10.1080/09583157.2012.694413]
[45]
Sharma P, Bhatt D, Zaidi MG, Saradhi PP, Khanna PK, Arora S. Silver nanoparticle-mediated enhancement in growth and antioxidant status of Brassica juncea. Appl Biochem Biotechnol 2012; 167(8): 2225-33.
[http://dx.doi.org/10.1007/s12010-012-9759-8] [PMID: 22692847]
[http://dx.doi.org/10.1007/s12010-012-9759-8] [PMID: 22692847]
[46]
Sheik S, Chandrashekar KR. Antimicrobial and antioxidant activities of Kingiodendron pinnatum (DC.) Harms and Humboldtia brunonis Wallich: endemic plants of the Western Ghats of India. J Natl Sci Found Sri Lanka 2014; 42(4)
[http://dx.doi.org/10.4038/jnsfsr.v42i4.7729]
[http://dx.doi.org/10.4038/jnsfsr.v42i4.7729]
[47]
Siddiqui ZA. PGPR: prospective biocontrol agents of plant pathogens.PGPR: Biocontrol and Biofertilization.Dordrecht: Springer 2005; pp. 111-42.
[48]
Khan N, Bano A, Shahid MA, Nasim W, Babar MA. Interaction between PGPR and PGR for water conservation and plant growth attributes under drought condition. Biologia 2018; 73(11): 1083-98.
[http://dx.doi.org/10.2478/s11756-018-0127-1]
[http://dx.doi.org/10.2478/s11756-018-0127-1]
[49]
Stefan MA, Munteanu N, Stoleru V, Mihasan MA. Effects of inoculation with plant growth promoting rhizobacteria on photosynthesis, antioxidant status and yield of runner bean. Rom Biotechnol Lett 2013; 18(2): 8132-43.
[50]
Thangavelu R, Palaniswami A, Doraiswamy S, Velazhahan R. The effect of Pseudomonas fluorescens and Fusarium oxysporum f. sp. cubense on induction of defense enzymes and phenolics in banana. Biol Plant 2003; 46(1): 107-12.
[http://dx.doi.org/10.1023/A:1022374520121]
[http://dx.doi.org/10.1023/A:1022374520121]
[51]
Vacheron J, Desbrosses G, Bouffaud ML, et al. Plant growth-promoting rhizobacteria and root system functioning. Front Plant Sci 2013; 4: 356.
[http://dx.doi.org/10.3389/fpls.2013.00356] [PMID: 24062756]
[http://dx.doi.org/10.3389/fpls.2013.00356] [PMID: 24062756]
[52]
Warwate SI, Kandoliya UK, Bhadja NV, Golakiya BA. The Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Biochemical Parameters of Coriander (Coriandrum sativum L.) Seedling. Int J Curr Microbiol Appl Sci 2017; 6(3): 1935-44.
[http://dx.doi.org/10.20546/ijcmas.2017.603.220]
[http://dx.doi.org/10.20546/ijcmas.2017.603.220]
[53]
Khan N, Bano A, Babar MA. The stimulatory effects of plant growth promoting rhizobacteria and plant growth regulators on wheat physiology grown in sandy soil. Arch Microbiol 2019; 201(6): 769-85.
[http://dx.doi.org/10.1007/s00203-019-01644-w]
[http://dx.doi.org/10.1007/s00203-019-01644-w]
[54]
Khan N, Ali S, Shahid MA, Kharabian-Masouleh A. Advances in detection of stress tolerance in plants through metabolomics approaches. Plant Omics 2017; 10(3): 153.
[http://dx.doi.org/10.21475/poj.10.03.17.pne600]
[http://dx.doi.org/10.21475/poj.10.03.17.pne600]
[55]
Dobbelaere S, Vanderleyden J, Okon Y. Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit Rev Plant Sci 2003; 22(2): 107-49.
[http://dx.doi.org/10.1080/713610853]
[http://dx.doi.org/10.1080/713610853]
[56]
Khan N, Bano A. Effects of exogenously applied salicylic acid and putrescine alone and in combination with rhizobacteria on the phytoremediation of heavy metals and chickpea growth in sandy soil. Int J Phytoremediation 2018; 20(5): 405-14.
[http://dx.doi.org/10.1080/15226514.2017.1381940]
[http://dx.doi.org/10.1080/15226514.2017.1381940]
[57]
Balal RM, Shahid MA, Javaid MM, et al. Chitosan alleviates phytotoxicity caused by boron through augmented polyamine metabolism and antioxidant activities and reduced boron concentration in Cucumis sativus L. Acta Physiol Plant 2017; 39(1): 31.
[http://dx.doi.org/10.1007/s11738-016-2335-z]
[http://dx.doi.org/10.1007/s11738-016-2335-z]
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