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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

罗汉果糖苷V通过逆转EMT和破坏细胞骨架抑制高血糖诱导的肺癌细胞转移。

卷 19, 期 11, 2019

页: [885 - 895] 页: 11

弟呕挨: 10.2174/1568009619666190619154240

价格: $65

摘要

背景：糖尿病（DM）加速了肺癌的进展。高血糖是DM的关键特征，可促进肺癌转移。罗汉果甙V是罗汉果（Siraitia grosvenorii）的三萜糖苷。有趣的是，罗汉果苷V不仅具有抗糖尿病作用，而且还具有抗肿瘤作用。 目的：在这项研究中，我们研究了罗汉果苷V在高血糖培养的肺癌细胞中的转移效率。 方法：分别在正常血糖（5.5mM葡萄糖）和高血糖症（25mM葡萄糖）中培养两个肺癌细胞系A549和H1299。 MTT法检测细胞增殖，Transwell法检测侵袭，伤口愈合法检测迁移，Phalloidin-TRITC法检测细胞骨架，Western blot法检测EMT标志物和Rho-GTPase家族蛋白的表达。 结果：与正常血糖相比，高血糖促进了A549和H1299细胞的侵袭和迁移。罗汉果苷V抑制高血糖引起的侵袭和迁移。高血糖症可促进上皮-间质转化（EMT），而罗汉果苷V可通过上调E-钙黏着蛋白表达和下调N-钙黏着蛋白，波形蛋白，蜗牛的表达来逆转这一过程。此外，在高血糖条件下，罗汉果苷V使微丝断裂并降低了Rho A，Rac1，Cdc42和p-PAK1的表达。 结论：这些结果表明罗汉果苷V通过逆转EMT和破坏细胞骨架来抑制高血糖诱导的肺癌细胞迁移和侵袭。

关键词: 罗汉果甙V，高血糖，转移，EMT，细胞骨架，肺癌。

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