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Medicinal Chemistry

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ISSN (Print): 1573-4064
ISSN (Online): 1875-6638

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Research Article

Polyethylene Glycol Acts as a Mechanistic Stabilizer of L-asparaginase: A Computational Probing

Author(s): Rajashekar Sindhu, Hanumanthappa Pradeep and Haravey K. Manonmani*

Volume 15, Issue 6, 2019

Page: [705 - 714] Pages: 10

DOI: 10.2174/1573406415666190206232816

Price: $65

Abstract

Background: L-asparaginase (L-ASN) is an anti-cancer enzyme therapeutic drug that exerts cytotoxicity via inhibition of protein synthesis through depletion of L-asparagine in the tumor microenvironment. The therapeutic performance of the native drug is partial due to the associated instability, reduced half-life and immunogenic complications.

Objective: In this study, we attempted the modification of recombinant L-asparaginase with PEG and an integrated computational strategy to probe the PEGylation in the protein to understand the biological stability/activity imparted by PEG.

Methods: In vitro PEGylation of recombinant L-ASN was carried out and further evaluated in silico.

Results: PEGylation enhanced thermal and pH activities with extended serum half-life and resistance to proteases compared to the native enzyme. The molecular dynamics analysis revealed intricate interactions required in the coupling of PEG to L-asparaginase to bestow stronger binding affinity of L-asparagine moiety towards L-asparaginase. PEG-asparagine complex ensured stable conformation over both the native protein and asparagine-protein complex thus elucidating the PEG-induced stable conformation in the protein. PEG mechanistically stabilized L-asparaginase through inducing pocket modification at the receptor to adapt to the cavity.

Conclusion: The study provides the rationale of PEGylation in imparting the stability towards Lasparaginase which would expand the potential application of L-asparaginase enzyme for the effective treatment of cancer.

Keywords: L-asparaginase, Polyethylene glycation, therapeutic drug, biological stability, cytotoxicity, computation.

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