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Current Pharmaceutical Analysis

Editor-in-Chief

ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Research Article

Comparison Study on the Contents of Eight Flavonoids in three Different Processed Products of Scutellariae Radix using Ultra-high Performance Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry

Author(s): Yuedong Yang, Hao Zhi, Baofei Yan, Yi Tian, Jianping Shen, Xingyu Zhu* and Zhipeng Li*

Volume 16, Issue 6, 2020

Page: [690 - 697] Pages: 8

DOI: 10.2174/1573412915666190206124150

Price: $65

Abstract

Background: The simultaneous determination of multiple components in a sample is an important factor in the quality control of traditional Chinese medicines and can give an indication of potential clinical applications.

Introduction: A rapid and sensitive method has been introduced for the simultaneous quantitative analysis of eight bioactive flavonoid constituents from Scutellariae Radix using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry.

Methods: The separation was performed on a Waters Acquity UPLC C18 column (2.1 mm×100 mm, 1.7 μm), under optimized mass spectrometry conditions, with a flow rate of 0.3 mL/min. The column temperature was maintained at 35°C and the injection volume was 3 μL.

Result: The method showed a good linear relationship of each component; all R2 values were above 0.9990 in the experiment. The RSDs of the precision test, repeatability test, stability test and recovery test were all not more than 2.86 %. We found that the total percentage amounts of the eight flavonoids were 22.19%, 18.63% and 10.86% in Raw Scutellariae Radix (RSR), Wine Scutellaria Radix (WSR) and Scutellaria Radix Charcoal (SRC) respectively.

Conclusion: The method was successfully applied to the simultaneous determination of the eight bioactive flavonoids of Raw Scutellariae Radix, Wine Scutellaria Radix and Scutellaria Radix Charcoal.

Keywords: Scutellariae radix, processing, UHPLC-TQ-MS/MS, quantification, flavonoids, quality control.

Graphical Abstract

[1]
Chinese Pharmacopoeia Commission. People's Republic of China Pharmacopoeia(1st section). Bei jing: The Medicine Science and Technology Press of China, 2015.
[2]
Waisundara, ; V.Y, Siu.; Sing, YungHsu.; Annie, Huang.; Dejian, Tan.; Benny, K. H. Baicalin upregulates the genetic expression of antioxidant enzymes in Type-2 diabetic Goto-Kakizaki rats. Life Sci., 2011, 88(23-24), 1016-1025.
[http://dx.doi.org/10.1016/j.lfs.2011.03.009]
[3]
Liu, ; Yu-min, ; Wang, ; Xiong, Nawaz.; Ahmed, Kong.; Zhao-hong, Hong.; Yan, Wang.; Chang-hua, Zhang.; , Jun-jian. Wogonin ameliorates lipotoxicity-induced apoptosis of cultured vascular smooth muscle cells via interfering with DAG-PKC pathway. Acta Pharmacol. Sin., 2011, 32(12), 1475-1482.
[http://dx.doi.org/10.1038/aps.2011.120]
[4]
Kong, ; E.K.C., Yu.; Shan, Sanderson.; John, E.Chen.; Kar-Bik, Huang.; Yu, Yu.; Cheuk-Man, A novel anti-fibrotic agent, baicalein, for the treatment of myocardial fibrosis in spontaneously hypertensive rats. Eur. J. Pharmacol., 2011, 658(2-3), 175-181.
[http://dx.doi.org/10.1016/j.ejphar.2011.02.033]
[5]
Wen, Y-F.; Zhao, J-Q.; Bhadauria, M.N. Satendra Kumar Baicalin prevents cadmium induced hepatic cytotoxicity, oxidative stress and histomorphometric alterations. Exp. Toxicol. Pathol., 2013, 65(1-2), 189-196.
[http://dx.doi.org/10.1016/j.etp.2011.08.005]
[6]
Li-Weber, M. New therapeutic aspects of flavones: The anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat. Rev., 2009, 35(1), 57-68.
[http://dx.doi.org/10.1016/j.ctrv.2008.09.005]
[7]
Li, Y.; Zhuang, P.; Shen, B.; Zhang, Y.; Shen, J. Baicalin promotes neuronal differentiation of neural stem/progenitor cells through modulating p-stat3 and bHLH family protein expression. Brain Res., 2012, 1429, 36-42.
[http://dx.doi.org/10.1016/j.brainres.2011.10.030]
[8]
Jiang, M.; Porat-Shliom, Y.; Pei, Z.; Cheng, Y.; Xiang, L.; Sommers, K.; Li, Q.; Gillardon, F.; Hengerer, B.; Berlinicke, C.; Smith, W.W.; Zack, D.J.; Poirier, M.A.; Ross, C.A.; Duan, W. Baicalein reduces E46K alpha-synuclein aggregation in vitro and protects cells against E46K alpha-synuclein toxicity in cell models of familiar Parkinsonism. J. Neurochem., 2010, 114(2), 419-429.
[http://dx.doi.org/10.1111/j.1471-4159.2010.06752.x]
[9]
Yang, Y.Z.; Tang, Y.Z.; Liu, Y.H. Wogonoside displays anti-inflammatory effects through modulating inflammatory mediator expression using RAW264.7 cells. J. Ethnopharmacol., 2013, 148(1), 271-276.
[http://dx.doi.org/10.1016/j.jep.2013.04.025]
[10]
Li, B.Q.; Fu, T.; Dongyan, Y.; Mikovits, J.A.; Ruscetti, F.W.; Wang, J.M. Flavonoid baicalin inhibits HIV-1 infection at the level of viral entry. Biochem. Biophys. Res. Commun., 2000, 276(2), 534-538.
[http://dx.doi.org/10.1006/bbrc.2000.3485]
[11]
Lee, I.K.; Kang, K.A.; Zhang, R.; Kim, B.J.; Kang, S.S.; Hyun, J.W. Mitochondria protection of baicalein against oxidative damage via induction of manganese superoxide dismutase. Environ. Toxicol. Pharmacol., 2011, 31(1), 233-241.
[http://dx.doi.org/10.1016/j.etap.2010.11.002]
[12]
Guo, M.; Zhang, N.; Li, D.; Liang, D.; Liu, Z.; Li, F.; Fu, Y.; Cao, Y.; Deng, X.; Yang, Z. Baicalin plays an anti-inflammatory role through reducing nuclear factor-kappa B and p38 phosphorylation in S. aureus-induced mastitis. Int. Immunopharmacol., 2013, 16(2), 125-130.
[http://dx.doi.org/10.1016/j.intimp.2013.03.006]
[13]
Serpa, R.; Franca, E.J.G.; Furlaneto-Maia, L.; Andrade, C.G.T.J.; Diniz, A.; Furlaneto, M.C. In vitro antifungal activity of the flavonoid baicalein against Candida species. J. Med. Microbiol., 2012, 61(12), 1704-1708.
[http://dx.doi.org/10.1099/jmm.0.047852-0]
[14]
Magnani, C.; Isaac, V.L.B.; Correa, M.A.; Salgado, H.R.N. Caffeic acid: a review of its potential use in medications and cosmetics. Anal. Methods, 2014, 6(10), 3203-3210.
[http://dx.doi.org/10.1039/C3AY41807C]
[15]
Chen, L.; Dou, J.; Su, Z.; Zhou, H.; Wang, H.; Zhou, W.; Guo, Q.; Zhou, C. Synergistic activity of baicalein with ribavirin against influenza A (H1N1) virus infections in cell culture and in mice. Antiviral Res., 2011, 91(3), 314-320.
[http://dx.doi.org/10.1016/j.antiviral.2011.07.008]
[16]
Cao, Y.; Dai, B.; Wang, Y.; Huang, S.; Xu, Y.; Cao, Y.; Gao, P.; Zhu, Z.; Jiang, Y. In vitro activity of baicalein against Candida albicans biofilms. Int. J. Antimicrob. Agents, 2008, 32(1), 73-77.
[http://dx.doi.org/10.1016/j.ijantimicag.2008.01.026]
[17]
The pharmaceutical administration of the ministry of health of the People’s Republic of China. National standard for preparation of Chinese herbal medicine; People's Medical Publishing House: Beijing, 1988.
[18]
Siddiqui, M.R.; AlOthman, Z.A.; Rahman, N. Analytical techniques in pharmaceutical analysis: A review. Arab. J. Chem., 2017, 10, S1409-S1421.
[http://dx.doi.org/10.1016/j.arabjc.2013.04.016]
[19]
Rahman, N.; Azmi, S.N.H.; Wu, H-F. The importance of impurity analysis in pharmaceutical products: an integrated approach. Accredit. Qual. Assur., 2006, 11(1-2), 69-74.
[http://dx.doi.org/10.1007/s00769-006-0095-y]
[20]
Siddiqui, Z.A.N.R.M. Review on Pharmaceutical Impurities. Stability Studies and Degradation Products: An Analytical Approach Reviews in Advanced Sciences & Engineering, 2013, 2(2), 155-166.
[21]
Ali, I.; Al-kindy, S.M.Z.; Suliman, F.O.; Alam, S.D. Fast analysis of flavonoids in apple juice on new generation halo column by SPE-HPLC. Anal. Methods, 2011, 3(12), 2836.
[http://dx.doi.org/10.1039/c1ay05496a]
[22]
Guo, X.; Wang, P.; Hu, X.; Fang, L.; Zhao, P.; Jian, Z.; Guo, X. Pharmacokinetic study of four components in rat plasma after oral administration of guanmaitong granule by UPLC-MS/MS. Curr. Pharm. Anal., 2018, 14(3), 223-232.
[http://dx.doi.org/10.2174/1573412913666170303105121]
[23]
Zhou, S. Q. S.; Yong, Tang.; and Weng, Naidong. Critical review of development, validation, and transfer for high throughput bioanalytical LC-MS/MS methods. Curr. Pharm. Anal., 2005, 1(1), 3-14.
[http://dx.doi.org/10.2174/1573412052953346]

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