摘要
背景:γ射线照射可显着诱导角膜广泛凋亡,并降低供体角膜的同种异体。并且X射线可能具有类似的生物效应。尚未评估X射线照射的角膜片的可行性和效果。 方法:采用SMILE手术采集不同剂量(10格雷(Gy),20 Gy ,50 Gy,100 Gy)X射线照射的角膜片。通过物理表征,苏木精和伊红(H-E)染色,Masson染色,TdT介导的dUTP缺口末端标记(TUNEL),细胞活力测定和透射电子显微镜(TEM)评估这些角膜薄片。我们选择最佳剂量(100Gy)来治疗角膜片是移植物。通过小切口飞秒激光辅助手术将人类移植物和新鲜的同种异体猴角膜片分别植入恒河猴。手术后进行临床检查和免疫染色。 结果:角膜片层透明度无明显变化,但角膜片层的吸光度增加。根据H-E和Masson的染色结果,照射对角膜胶原的影响很小。 TUNEL测定和细胞活力测定结果显示100Gy X射线照射导致角膜片层完全凋亡,这也通过TEM观察证实。在以下动物模型研究中,没有发生免疫反应或严重的炎症反应,并且宿主角膜在观察24周时保持透明度。并且CD4和CD8的表达在所有宿主角膜中均为阴性。 结论:X射线照射的角膜片可作为异种镶嵌的潜在材料,小切口飞秒激光辅助植入有可能成为一种新的角膜移植手术方法。
关键词: X射线照射,SMILE,飞秒激光,异种移植,恒河猴。
Current Molecular Medicine
Title:Small Incision Femtosecond Laser-assisted X-ray-irradiated Corneal Intrastromal Xenotransplantation in Rhesus Monkeys: A Preliminary Study
Volume: 18 Issue: 9
关键词: X射线照射,SMILE,飞秒激光,异种移植,恒河猴。
摘要: Background: Gamma-ray irradiation could significantly induce widespread apoptosis in corneas and reduced the allogenicity of donor cornea. And the X-rays may have similar biological effects. The feasibility and effects of X-ray-irradiated corneal lamellae have not been assessed yet.
Methods: Different doses (10 gray unit (Gy), 20 Gy, 50 Gy, 100 Gy) of X-ray irradiated corneal lamellae were collected from SMILE surgery. These corneal lamellae were assessed by physical characterization, hematoxylin and eosin (H-E) staining, Masson’s staining, TdT-mediated dUTP nick end labeling (TUNEL), cell viability assay and transmission electron microscopy (TEM). We selected the optimum dose (100Gy) to treat the corneal lamellae to be the grafts. The human grafts and fresh allogeneic monkey corneal lamellae were implanted into rhesus monkeys via the small incision femtosecond laser-assisted surgery, respectively. Clinical examinations and the immunostaining were performed after surgery.
Results: There were no significant changes in the transparency of the corneal lamellae, but the absorbency of the corneal lamellae was increased. According to the H-E and Masson’s staining results, irradiation had little impact on the corneal collagen. The TUNEL assay and cell viability assay results showed that 100Gy X-ray irradiation resulted in complete apoptosis in the corneal lamellae, which was also confirmed by TEM observations. In the following animal model study, no immune reactions or severe inflammatory responses occurred, and the host corneas maintained transparency for 24 weeks of observation. And the expression of CD4 and CD8 were negative in the all host corneas.
Conclusion: X-ray irradiated corneal lamellae could serve as a potential material for xenogeneic inlay, and the small incision femtosecond laser-assisted implantation has the potential to become a new corneal transplantation surgical approach.
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Small Incision Femtosecond Laser-assisted X-ray-irradiated Corneal Intrastromal Xenotransplantation in Rhesus Monkeys: A Preliminary Study, Current Molecular Medicine 2018; 18 (9) . https://dx.doi.org/10.2174/1566524019666190129123935
DOI https://dx.doi.org/10.2174/1566524019666190129123935 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
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