摘要
转基因小鼠已被广泛用于研究阿尔茨海默病病理学。 为了减少，改进和替换（3Rs）动物的数量，使用并优化离体培养物。 器官型脑切片是最有效的离体切片培养模型，保持大脑的三维结构并且最接近体内情况。 器官型脑切片培养已经使用了数十年，但主要是从出生后（第8-10天）老年大鼠或小鼠制备的。 最近的工作（包括我们的实验室）现在旨在培养来自成年小鼠（包括转基因小鼠）的器官型脑切片。 特别是在阿尔茨海默病研究中，来自成年转基因小鼠的脑切片将用于研究β-淀粉样斑块，tau病理学和胶质细胞活化。 本综述将总结使用成年小鼠的器官型脑切片培养模拟阿尔茨海默病的研究，并将突出使用该技术的优势和缺陷。
关键词: 器官型脑切片，转基因，阿尔茨海默病，成年小鼠，切碎片，vibroslices。
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