摘要
组合产生的分子库已广泛用于鉴定新型生物活性化合物。已经提出了越来越复杂的技术解决方案来简化和加速这样的过程,扩大了化学多样性的空间,并且增加了选择具有针对治疗相关目标的特异性和有效活性的新分子实体的前景。在这种情况下,最初使用的是寡聚肽的随机混合物,并且自25年以来,它们代表了生物活性分子的连续来源,其效力范围从亚nM到microM浓度。合成肽库仍被用作结构和化学上多样化的分子片段的起始“合成肉汤”,可以从中提取先导化合物并进行进一步修饰。据报道,成千上万的研究描述了具有不同复杂性并嫁接到不同结构支架上的合成肽组合混合物的应用,以鉴定新化合物,这些化合物已得到进一步开发,并已在相关疾病的体内模型中进行了测试。我们简要回顾了一些用于文库制备和筛选的最常用方法,最近的案例研究出现在文献中,化合物至少已在动物或类似模型中进行了体内测试。生物技术,工程学和计算机科学领域的最新技术进步为促进新生物活性肽的发现提出了新的选择。在这种情况下,我们在这里期望一种新的方法来设计简单但针对性的三肽文库,以对抗治疗靶标的可药腔及其与现有方法的互补。
关键词: 肽库,体内测试,分对偶联重组,固相肽合成,随机混合物,迭代解卷积。
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